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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-150376149-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=150376149&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 150376149,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001371623.1",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "NM_001371623.1",
          "protein_id": "NP_001358552.1",
          "transcript_support_level": null,
          "aa_start": 654,
          "aa_end": null,
          "aa_length": 1489,
          "cds_start": 1961,
          "cds_end": null,
          "cds_length": 4470,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000643257.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001371623.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000643257.2",
          "protein_id": "ENSP00000493815.1",
          "transcript_support_level": null,
          "aa_start": 654,
          "aa_end": null,
          "aa_length": 1489,
          "cds_start": 1961,
          "cds_end": null,
          "cds_length": 4470,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001371623.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000643257.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000504761.6",
          "protein_id": "ENSP00000421655.2",
          "transcript_support_level": 1,
          "aa_start": 654,
          "aa_end": null,
          "aa_length": 1488,
          "cds_start": 1961,
          "cds_end": null,
          "cds_length": 4467,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000504761.6"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1730C>T",
          "hgvs_p": "p.Ala577Val",
          "transcript": "ENST00000323668.11",
          "protein_id": "ENSP00000325223.6",
          "transcript_support_level": 1,
          "aa_start": 577,
          "aa_end": null,
          "aa_length": 1411,
          "cds_start": 1730,
          "cds_end": null,
          "cds_length": 4236,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000323668.11"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000394269.7",
          "protein_id": "ENSP00000377811.3",
          "transcript_support_level": 1,
          "aa_start": 654,
          "aa_end": null,
          "aa_length": 958,
          "cds_start": 1961,
          "cds_end": null,
          "cds_length": 2877,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000394269.7"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000930571.1",
          "protein_id": "ENSP00000600630.1",
          "transcript_support_level": null,
          "aa_start": 654,
          "aa_end": null,
          "aa_length": 1558,
          "cds_start": 1961,
          "cds_end": null,
          "cds_length": 4677,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930571.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000930555.1",
          "protein_id": "ENSP00000600614.1",
          "transcript_support_level": null,
          "aa_start": 654,
          "aa_end": null,
          "aa_length": 1525,
          "cds_start": 1961,
          "cds_end": null,
          "cds_length": 4578,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930555.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000930560.1",
          "protein_id": "ENSP00000600619.1",
          "transcript_support_level": null,
          "aa_start": 654,
          "aa_end": null,
          "aa_length": 1525,
          "cds_start": 1961,
          "cds_end": null,
          "cds_length": 4578,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930560.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000930554.1",
          "protein_id": "ENSP00000600613.1",
          "transcript_support_level": null,
          "aa_start": 654,
          "aa_end": null,
          "aa_length": 1524,
          "cds_start": 1961,
          "cds_end": null,
          "cds_length": 4575,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930554.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.2039C>T",
          "hgvs_p": "p.Ala680Val",
          "transcript": "ENST00000930565.1",
          "protein_id": "ENSP00000600624.1",
          "transcript_support_level": null,
          "aa_start": 680,
          "aa_end": null,
          "aa_length": 1514,
          "cds_start": 2039,
          "cds_end": null,
          "cds_length": 4545,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930565.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000930570.1",
          "protein_id": "ENSP00000600629.1",
          "transcript_support_level": null,
          "aa_start": 654,
          "aa_end": null,
          "aa_length": 1509,
          "cds_start": 1961,
          "cds_end": null,
          "cds_length": 4530,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930570.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000930586.1",
          "protein_id": "ENSP00000600645.1",
          "transcript_support_level": null,
          "aa_start": 654,
          "aa_end": null,
          "aa_length": 1508,
          "cds_start": 1961,
          "cds_end": null,
          "cds_length": 4527,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "NM_001135243.2",
          "protein_id": "NP_001128715.1",
          "transcript_support_level": null,
          "aa_start": 654,
          "aa_end": null,
          "aa_length": 1488,
          "cds_start": 1961,
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          "cds_length": 4467,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001135243.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000377797.7",
          "protein_id": "ENSP00000367028.4",
          "transcript_support_level": 5,
          "aa_start": 654,
          "aa_end": null,
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          "cds_start": 1961,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "A",
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          "protein_coding": true,
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          ],
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          "intron_rank": null,
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          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000513346.5",
          "protein_id": "ENSP00000427484.1",
          "transcript_support_level": 5,
          "aa_start": 654,
          "aa_end": null,
          "aa_length": 1488,
          "cds_start": 1961,
          "cds_end": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000513346.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000930558.1",
          "protein_id": "ENSP00000600617.1",
          "transcript_support_level": null,
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          "aa_length": 1488,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "A",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000930544.1",
          "protein_id": "ENSP00000600603.1",
          "transcript_support_level": null,
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        {
          "aa_ref": "A",
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          "exon_rank_end": null,
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          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000930556.1",
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        },
        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000930557.1",
          "protein_id": "ENSP00000600616.1",
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930557.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCOF1",
          "gene_hgnc_id": 11654,
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val",
          "transcript": "ENST00000930575.1",
          "protein_id": "ENSP00000600634.1",
          "transcript_support_level": null,
          "aa_start": 654,
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          "cdna_start": null,
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          "biotype": "retained_intron",
          "feature": "ENST00000515035.5"
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      ],
      "gene_symbol": "TCOF1",
      "gene_hgnc_id": 11654,
      "dbsnp": "rs201843484",
      "frequency_reference_population": 0.000111515874,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 180,
      "gnomad_exomes_af": 0.000118342,
      "gnomad_genomes_af": 0.0000459758,
      "gnomad_exomes_ac": 173,
      "gnomad_genomes_ac": 7,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.17227956652641296,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.07999999821186066,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.336,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1144,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.31,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.269,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.08,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -14,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Moderate,BP6_Very_Strong,BS2",
      "acmg_by_gene": [
        {
          "score": -14,
          "benign_score": 14,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6_Very_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_001371623.1",
          "gene_symbol": "TCOF1",
          "hgnc_id": 11654,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1961C>T",
          "hgvs_p": "p.Ala654Val"
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      ],
      "clinvar_disease": "Treacher Collins syndrome 1,not provided",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:2",
      "phenotype_combined": "not provided|Treacher Collins syndrome 1",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}