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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-172959989-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=172959989&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 172959989,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_016093.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "NM_016093.4",
          "protein_id": "NP_057177.1",
          "transcript_support_level": null,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000265100.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_016093.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000265100.6",
          "protein_id": "ENSP00000265100.2",
          "transcript_support_level": 1,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_016093.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000265100.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000519156.1",
          "protein_id": "ENSP00000430673.1",
          "transcript_support_level": 1,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 127,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 384,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000519156.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "NM_001317980.2",
          "protein_id": "NP_001304909.1",
          "transcript_support_level": null,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001317980.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "NM_001317981.2",
          "protein_id": "NP_001304910.1",
          "transcript_support_level": null,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001317981.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "NM_001317982.2",
          "protein_id": "NP_001304911.1",
          "transcript_support_level": null,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001317982.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000519239.5",
          "protein_id": "ENSP00000430147.1",
          "transcript_support_level": 2,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000519239.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000519974.5",
          "protein_id": "ENSP00000428177.1",
          "transcript_support_level": 3,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000519974.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000521476.5",
          "protein_id": "ENSP00000428223.1",
          "transcript_support_level": 2,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000521476.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000906540.1",
          "protein_id": "ENSP00000576599.1",
          "transcript_support_level": null,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906540.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000906541.1",
          "protein_id": "ENSP00000576600.1",
          "transcript_support_level": null,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906541.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000935963.1",
          "protein_id": "ENSP00000606022.1",
          "transcript_support_level": null,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 116,
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          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000935963.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000935964.1",
          "protein_id": "ENSP00000606023.1",
          "transcript_support_level": null,
          "aa_start": 39,
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          "cds_start": 116,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935964.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000935966.1",
          "protein_id": "ENSP00000606025.1",
          "transcript_support_level": null,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 116,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
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        },
        {
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 2,
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          "exon_count": 4,
          "intron_rank": null,
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          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000935967.1",
          "protein_id": "ENSP00000606026.1",
          "transcript_support_level": null,
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          "cds_start": 116,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000935967.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000935968.1",
          "protein_id": "ENSP00000606027.1",
          "transcript_support_level": null,
          "aa_start": 39,
          "aa_end": null,
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          "cds_start": 116,
          "cds_end": null,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        {
          "aa_ref": "R",
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          "canonical": false,
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          "strand": true,
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          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000935969.1",
          "protein_id": "ENSP00000606028.1",
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        {
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          "intron_rank": null,
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          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
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          "transcript": "ENST00000971027.1",
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          "biotype": "protein_coding",
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        },
        {
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          ],
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          "gene_symbol": "RPL26L1",
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          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000971028.1",
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          "biotype": "protein_coding",
          "feature": "ENST00000971028.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26L1",
          "gene_hgnc_id": 17050,
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln",
          "transcript": "ENST00000935970.1",
          "protein_id": "ENSP00000606029.1",
          "transcript_support_level": null,
          "aa_start": 39,
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          "aa_length": 143,
          "cds_start": 116,
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          "cds_length": 432,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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        {
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        },
        {
          "aa_ref": "?",
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            "synonymous_variant"
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          "hgvs_p": "p.???39???",
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        {
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          "biotype": "pseudogene",
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        {
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          "gene_symbol": "RPL26L1-AS1",
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          "hgvs_c": "n.87C>T",
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          "transcript": "ENST00000758381.1",
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          "biotype": "pseudogene",
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        },
        {
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          "protein_coding": false,
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          "gene_symbol": "RPL26L1-AS1",
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          "transcript": "ENST00000758382.1",
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          "biotype": "pseudogene",
          "feature": "ENST00000758382.1"
        }
      ],
      "gene_symbol": "RPL26L1",
      "gene_hgnc_id": 17050,
      "dbsnp": "rs1389341083",
      "frequency_reference_population": 0.0000034202394,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.00000342024,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 5,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6770120859146118,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.658,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.275,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.14,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.862,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BS2",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_016093.4",
          "gene_symbol": "RPL26L1",
          "hgnc_id": 17050,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.116G>A",
          "hgvs_p": "p.Arg39Gln"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000758379.1",
          "gene_symbol": "RPL26L1-AS1",
          "hgnc_id": 55914,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.93C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}