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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-176881147-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=176881147&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 176881147,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_002115.3",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK3",
          "gene_hgnc_id": 4925,
          "hgvs_c": "c.2698G>C",
          "hgvs_p": "p.Glu900Gln",
          "transcript": "NM_002115.3",
          "protein_id": "NP_002106.2",
          "transcript_support_level": null,
          "aa_start": 900,
          "aa_end": null,
          "aa_length": 923,
          "cds_start": 2698,
          "cds_end": null,
          "cds_length": 2772,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000292432.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002115.3"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK3",
          "gene_hgnc_id": 4925,
          "hgvs_c": "c.2698G>C",
          "hgvs_p": "p.Glu900Gln",
          "transcript": "ENST00000292432.10",
          "protein_id": "ENSP00000292432.5",
          "transcript_support_level": 1,
          "aa_start": 900,
          "aa_end": null,
          "aa_length": 923,
          "cds_start": 2698,
          "cds_end": null,
          "cds_length": 2772,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002115.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000292432.10"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK3",
          "gene_hgnc_id": 4925,
          "hgvs_c": "n.1710G>C",
          "hgvs_p": null,
          "transcript": "ENST00000506834.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000506834.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK3",
          "gene_hgnc_id": 4925,
          "hgvs_c": "c.2698G>C",
          "hgvs_p": "p.Glu900Gln",
          "transcript": "ENST00000874508.1",
          "protein_id": "ENSP00000544567.1",
          "transcript_support_level": null,
          "aa_start": 900,
          "aa_end": null,
          "aa_length": 923,
          "cds_start": 2698,
          "cds_end": null,
          "cds_length": 2772,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874508.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK3",
          "gene_hgnc_id": 4925,
          "hgvs_c": "c.2692G>C",
          "hgvs_p": "p.Glu898Gln",
          "transcript": "ENST00000970949.1",
          "protein_id": "ENSP00000641008.1",
          "transcript_support_level": null,
          "aa_start": 898,
          "aa_end": null,
          "aa_length": 921,
          "cds_start": 2692,
          "cds_end": null,
          "cds_length": 2766,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970949.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK3",
          "gene_hgnc_id": 4925,
          "hgvs_c": "c.2692G>C",
          "hgvs_p": "p.Glu898Gln",
          "transcript": "ENST00000970955.1",
          "protein_id": "ENSP00000641014.1",
          "transcript_support_level": null,
          "aa_start": 898,
          "aa_end": null,
          "aa_length": 921,
          "cds_start": 2692,
          "cds_end": null,
          "cds_length": 2766,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970955.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK3",
          "gene_hgnc_id": 4925,
          "hgvs_c": "c.2674G>C",
          "hgvs_p": "p.Glu892Gln",
          "transcript": "ENST00000970953.1",
          "protein_id": "ENSP00000641012.1",
          "transcript_support_level": null,
          "aa_start": 892,
          "aa_end": null,
          "aa_length": 915,
          "cds_start": 2674,
          "cds_end": null,
          "cds_length": 2748,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970953.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK3",
          "gene_hgnc_id": 4925,
          "hgvs_c": "c.2593G>C",
          "hgvs_p": "p.Glu865Gln",
          "transcript": "ENST00000970952.1",
          "protein_id": "ENSP00000641011.1",
          "transcript_support_level": null,
          "aa_start": 865,
          "aa_end": null,
          "aa_length": 888,
          "cds_start": 2593,
          "cds_end": null,
          "cds_length": 2667,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970952.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK3",
          "gene_hgnc_id": 4925,
          "hgvs_c": "c.2578G>C",
          "hgvs_p": "p.Glu860Gln",
          "transcript": "ENST00000970951.1",
          "protein_id": "ENSP00000641010.1",
          "transcript_support_level": null,
          "aa_start": 860,
          "aa_end": null,
          "aa_length": 883,
          "cds_start": 2578,
          "cds_end": null,
          "cds_length": 2652,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970951.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK3",
          "gene_hgnc_id": 4925,
          "hgvs_c": "c.2464G>C",
          "hgvs_p": "p.Glu822Gln",
          "transcript": "ENST00000970948.1",
          "protein_id": "ENSP00000641007.1",
          "transcript_support_level": null,
          "aa_start": 822,
          "aa_end": null,
          "aa_length": 845,
          "cds_start": 2464,
          "cds_end": null,
          "cds_length": 2538,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970948.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK3",
          "gene_hgnc_id": 4925,
          "hgvs_c": "c.2464G>C",
          "hgvs_p": "p.Glu822Gln",
          "transcript": "ENST00000970950.1",
          "protein_id": "ENSP00000641009.1",
          "transcript_support_level": null,
          "aa_start": 822,
          "aa_end": null,
          "aa_length": 845,
          "cds_start": 2464,
          "cds_end": null,
          "cds_length": 2538,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970950.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK3",
          "gene_hgnc_id": 4925,
          "hgvs_c": "c.1930G>C",
          "hgvs_p": "p.Glu644Gln",
          "transcript": "ENST00000970954.1",
          "protein_id": "ENSP00000641013.1",
          "transcript_support_level": null,
          "aa_start": 644,
          "aa_end": null,
          "aa_length": 667,
          "cds_start": 1930,
          "cds_end": null,
          "cds_length": 2004,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970954.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "UNC5A",
          "gene_hgnc_id": 12567,
          "hgvs_c": "c.*1261C>G",
          "hgvs_p": null,
          "transcript": "NM_133369.3",
          "protein_id": "NP_588610.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 842,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2529,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000329542.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_133369.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "UNC5A",
          "gene_hgnc_id": 12567,
          "hgvs_c": "c.*1261C>G",
          "hgvs_p": null,
          "transcript": "ENST00000329542.9",
          "protein_id": "ENSP00000332737.4",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 842,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2529,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_133369.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000329542.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "UNC5A",
          "gene_hgnc_id": 12567,
          "hgvs_c": "c.*1261C>G",
          "hgvs_p": null,
          "transcript": "ENST00000509580.2",
          "protein_id": "ENSP00000421795.2",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 898,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2697,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000509580.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "UNC5A",
          "gene_hgnc_id": 12567,
          "hgvs_c": "c.*1261C>G",
          "hgvs_p": null,
          "transcript": "XM_006714928.2",
          "protein_id": "XP_006714991.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 898,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2697,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006714928.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK3",
          "gene_hgnc_id": 4925,
          "hgvs_c": "n.*155G>C",
          "hgvs_p": null,
          "transcript": "ENST00000514666.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000514666.1"
        }
      ],
      "gene_symbol": "HK3",
      "gene_hgnc_id": 4925,
      "dbsnp": "rs546596860",
      "frequency_reference_population": 0.000006564178,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": 0.00000656418,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8178107142448425,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.772,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.1836,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": 0.05,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 4.659,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_002115.3",
          "gene_symbol": "HK3",
          "hgnc_id": 4925,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.2698G>C",
          "hgvs_p": "p.Glu900Gln"
        },
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_133369.3",
          "gene_symbol": "UNC5A",
          "hgnc_id": 12567,
          "effects": [
            "downstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.*1261C>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}