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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-177337418-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=177337418&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 177337418,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_006816.3",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "c.620G>A",
          "hgvs_p": "p.Arg207His",
          "transcript": "NM_006816.3",
          "protein_id": "NP_006807.1",
          "transcript_support_level": null,
          "aa_start": 207,
          "aa_end": null,
          "aa_length": 356,
          "cds_start": 620,
          "cds_end": null,
          "cds_length": 1071,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000303127.12",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_006816.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "c.620G>A",
          "hgvs_p": "p.Arg207His",
          "transcript": "ENST00000303127.12",
          "protein_id": "ENSP00000303366.7",
          "transcript_support_level": 1,
          "aa_start": 207,
          "aa_end": null,
          "aa_length": 356,
          "cds_start": 620,
          "cds_end": null,
          "cds_length": 1071,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_006816.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000303127.12"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "c.746G>A",
          "hgvs_p": "p.Arg249His",
          "transcript": "ENST00000883396.1",
          "protein_id": "ENSP00000553455.1",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 746,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883396.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "c.620G>A",
          "hgvs_p": "p.Arg207His",
          "transcript": "ENST00000883394.1",
          "protein_id": "ENSP00000553453.1",
          "transcript_support_level": null,
          "aa_start": 207,
          "aa_end": null,
          "aa_length": 366,
          "cds_start": 620,
          "cds_end": null,
          "cds_length": 1101,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883394.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "c.641G>A",
          "hgvs_p": "p.Arg214His",
          "transcript": "ENST00000883395.1",
          "protein_id": "ENSP00000553454.1",
          "transcript_support_level": null,
          "aa_start": 214,
          "aa_end": null,
          "aa_length": 363,
          "cds_start": 641,
          "cds_end": null,
          "cds_length": 1092,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883395.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "c.620G>A",
          "hgvs_p": "p.Arg207His",
          "transcript": "ENST00000883393.1",
          "protein_id": "ENSP00000553452.1",
          "transcript_support_level": null,
          "aa_start": 207,
          "aa_end": null,
          "aa_length": 347,
          "cds_start": 620,
          "cds_end": null,
          "cds_length": 1044,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883393.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "c.620G>A",
          "hgvs_p": "p.Arg207His",
          "transcript": "ENST00000515209.5",
          "protein_id": "ENSP00000423998.1",
          "transcript_support_level": 3,
          "aa_start": 207,
          "aa_end": null,
          "aa_length": 325,
          "cds_start": 620,
          "cds_end": null,
          "cds_length": 978,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000515209.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "c.620G>A",
          "hgvs_p": "p.Arg207His",
          "transcript": "ENST00000694905.1",
          "protein_id": "ENSP00000511580.1",
          "transcript_support_level": null,
          "aa_start": 207,
          "aa_end": null,
          "aa_length": 317,
          "cds_start": 620,
          "cds_end": null,
          "cds_length": 954,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000694905.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "c.599G>A",
          "hgvs_p": "p.Arg200His",
          "transcript": "ENST00000502560.5",
          "protein_id": "ENSP00000425229.1",
          "transcript_support_level": 3,
          "aa_start": 200,
          "aa_end": null,
          "aa_length": 287,
          "cds_start": 599,
          "cds_end": null,
          "cds_length": 864,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000502560.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "c.407G>A",
          "hgvs_p": "p.Arg136His",
          "transcript": "ENST00000513877.1",
          "protein_id": "ENSP00000427377.1",
          "transcript_support_level": 2,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 190,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 575,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000513877.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "c.513+288G>A",
          "hgvs_p": null,
          "transcript": "ENST00000514458.5",
          "protein_id": "ENSP00000424132.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 202,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 611,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000514458.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "n.649G>A",
          "hgvs_p": null,
          "transcript": "ENST00000502721.6",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000502721.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "n.813G>A",
          "hgvs_p": null,
          "transcript": "ENST00000694903.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000694903.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "n.2222G>A",
          "hgvs_p": null,
          "transcript": "ENST00000694904.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000694904.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LMAN2",
          "gene_hgnc_id": 16986,
          "hgvs_c": "n.*17G>A",
          "hgvs_p": null,
          "transcript": "ENST00000506310.1",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000506310.1"
        }
      ],
      "gene_symbol": "LMAN2",
      "gene_hgnc_id": 16986,
      "dbsnp": "rs763732580",
      "frequency_reference_population": 0.000004339191,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 7,
      "gnomad_exomes_af": 0.0000041068,
      "gnomad_genomes_af": 0.0000065697,
      "gnomad_exomes_ac": 6,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9607951641082764,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.783,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9222,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.14,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.834,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM2",
            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_006816.3",
          "gene_symbol": "LMAN2",
          "hgnc_id": 16986,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.620G>A",
          "hgvs_p": "p.Arg207His"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}