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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-178211964-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=178211964&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 178211964,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000308158.10",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.1310A>C",
          "hgvs_p": "p.Glu437Ala",
          "transcript": "NM_153373.4",
          "protein_id": "NP_699204.1",
          "transcript_support_level": null,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 450,
          "cds_start": 1310,
          "cds_end": null,
          "cds_length": 1353,
          "cdna_start": 1562,
          "cdna_end": null,
          "cdna_length": 2081,
          "mane_select": "ENST00000308158.10",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.1310A>C",
          "hgvs_p": "p.Glu437Ala",
          "transcript": "ENST00000308158.10",
          "protein_id": "ENSP00000310978.5",
          "transcript_support_level": 1,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 450,
          "cds_start": 1310,
          "cds_end": null,
          "cds_length": 1353,
          "cdna_start": 1562,
          "cdna_end": null,
          "cdna_length": 2081,
          "mane_select": "NM_153373.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "n.*698A>C",
          "hgvs_p": null,
          "transcript": "ENST00000474052.5",
          "protein_id": "ENSP00000423806.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1539,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "n.1951A>C",
          "hgvs_p": null,
          "transcript": "ENST00000494126.6",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2297,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "n.*698A>C",
          "hgvs_p": null,
          "transcript": "ENST00000474052.5",
          "protein_id": "ENSP00000423806.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1539,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.1352A>C",
          "hgvs_p": "p.Glu451Ala",
          "transcript": "ENST00000323594.9",
          "protein_id": "ENSP00000321290.5",
          "transcript_support_level": 5,
          "aa_start": 451,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 1352,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": 1432,
          "cdna_end": null,
          "cdna_length": 3278,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.1187A>C",
          "hgvs_p": "p.Glu396Ala",
          "transcript": "NM_001278346.1",
          "protein_id": "NP_001265275.1",
          "transcript_support_level": null,
          "aa_start": 396,
          "aa_end": null,
          "aa_length": 409,
          "cds_start": 1187,
          "cds_end": null,
          "cds_length": 1230,
          "cdna_start": 2405,
          "cdna_end": null,
          "cdna_length": 2921,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.1340A>C",
          "hgvs_p": "p.Glu447Ala",
          "transcript": "XM_011534669.3",
          "protein_id": "XP_011532971.1",
          "transcript_support_level": null,
          "aa_start": 447,
          "aa_end": null,
          "aa_length": 460,
          "cds_start": 1340,
          "cds_end": null,
          "cds_length": 1383,
          "cdna_start": 1592,
          "cdna_end": null,
          "cdna_length": 2111,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.1337A>C",
          "hgvs_p": "p.Glu446Ala",
          "transcript": "XM_011534670.4",
          "protein_id": "XP_011532972.1",
          "transcript_support_level": null,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 459,
          "cds_start": 1337,
          "cds_end": null,
          "cds_length": 1380,
          "cdna_start": 2316,
          "cdna_end": null,
          "cdna_length": 2835,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.1328A>C",
          "hgvs_p": "p.Glu443Ala",
          "transcript": "XM_011534671.3",
          "protein_id": "XP_011532973.1",
          "transcript_support_level": null,
          "aa_start": 443,
          "aa_end": null,
          "aa_length": 456,
          "cds_start": 1328,
          "cds_end": null,
          "cds_length": 1371,
          "cdna_start": 1580,
          "cdna_end": null,
          "cdna_length": 2099,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.1307A>C",
          "hgvs_p": "p.Glu436Ala",
          "transcript": "XM_047417837.1",
          "protein_id": "XP_047273793.1",
          "transcript_support_level": null,
          "aa_start": 436,
          "aa_end": null,
          "aa_length": 449,
          "cds_start": 1307,
          "cds_end": null,
          "cds_length": 1350,
          "cdna_start": 2286,
          "cdna_end": null,
          "cdna_length": 2805,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.1184A>C",
          "hgvs_p": "p.Glu395Ala",
          "transcript": "XM_006714925.3",
          "protein_id": "XP_006714988.1",
          "transcript_support_level": null,
          "aa_start": 395,
          "aa_end": null,
          "aa_length": 408,
          "cds_start": 1184,
          "cds_end": null,
          "cds_length": 1227,
          "cdna_start": 1436,
          "cdna_end": null,
          "cdna_length": 1955,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.1067A>C",
          "hgvs_p": "p.Glu356Ala",
          "transcript": "XM_024446240.2",
          "protein_id": "XP_024302008.1",
          "transcript_support_level": null,
          "aa_start": 356,
          "aa_end": null,
          "aa_length": 369,
          "cds_start": 1067,
          "cds_end": null,
          "cds_length": 1110,
          "cdna_start": 1319,
          "cdna_end": null,
          "cdna_length": 1838,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.1064A>C",
          "hgvs_p": "p.Glu355Ala",
          "transcript": "XM_047417838.1",
          "protein_id": "XP_047273794.1",
          "transcript_support_level": null,
          "aa_start": 355,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": 1064,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": 2043,
          "cdna_end": null,
          "cdna_length": 2562,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.995A>C",
          "hgvs_p": "p.Glu332Ala",
          "transcript": "XM_011534674.3",
          "protein_id": "XP_011532976.1",
          "transcript_support_level": null,
          "aa_start": 332,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": 995,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": 1247,
          "cdna_end": null,
          "cdna_length": 1766,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.782A>C",
          "hgvs_p": "p.Glu261Ala",
          "transcript": "XM_024446243.2",
          "protein_id": "XP_024302011.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 274,
          "cds_start": 782,
          "cds_end": null,
          "cds_length": 825,
          "cdna_start": 3474,
          "cdna_end": null,
          "cdna_length": 3993,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.746A>C",
          "hgvs_p": "p.Glu249Ala",
          "transcript": "XM_024446245.2",
          "protein_id": "XP_024302013.1",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 746,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": 3471,
          "cdna_end": null,
          "cdna_length": 3990,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.743A>C",
          "hgvs_p": "p.Glu248Ala",
          "transcript": "XM_024446247.2",
          "protein_id": "XP_024302015.1",
          "transcript_support_level": null,
          "aa_start": 248,
          "aa_end": null,
          "aa_length": 261,
          "cds_start": 743,
          "cds_end": null,
          "cds_length": 786,
          "cdna_start": 1748,
          "cdna_end": null,
          "cdna_length": 2267,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.716A>C",
          "hgvs_p": "p.Glu239Ala",
          "transcript": "XM_024446250.2",
          "protein_id": "XP_024302018.1",
          "transcript_support_level": null,
          "aa_start": 239,
          "aa_end": null,
          "aa_length": 252,
          "cds_start": 716,
          "cds_end": null,
          "cds_length": 759,
          "cdna_start": 3441,
          "cdna_end": null,
          "cdna_length": 3960,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "n.503A>C",
          "hgvs_p": null,
          "transcript": "ENST00000393488.2",
          "protein_id": "ENSP00000377128.2",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1001,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "n.1255A>C",
          "hgvs_p": null,
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          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 731,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PHYKPL",
          "gene_hgnc_id": 28249,
          "hgvs_c": "c.*55A>C",
          "hgvs_p": null,
          "transcript": "XM_047417840.1",
          "protein_id": "XP_047273796.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 332,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 999,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1380,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "PHYKPL",
      "gene_hgnc_id": 28249,
      "dbsnp": "rs142181517",
      "frequency_reference_population": 6.840535e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84053e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.5800537467002869,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.632,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.2507,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": 0.03,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 3.329,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000308158.10",
          "gene_symbol": "PHYKPL",
          "hgnc_id": 28249,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1310A>C",
          "hgvs_p": "p.Glu437Ala"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}