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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-179825182-TGAA-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=179825182&ref=TGAA&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "message": null,
  "variants": [
    {
      "acmg_by_gene": [
        {
          "benign_score": 0,
          "criteria": [
            "PM4_Supporting"
          ],
          "effects": [
            "disruptive_inframe_deletion"
          ],
          "gene_symbol": "SQSTM1",
          "hgnc_id": 11280,
          "hgvs_c": "c.714_716delGAA",
          "hgvs_p": "p.Lys238del",
          "inheritance_mode": "AD,AR",
          "pathogenic_score": 1,
          "score": 1,
          "transcript": "NM_003900.5",
          "verdict": "Uncertain_significance"
        }
      ],
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM4_Supporting",
      "acmg_score": 1,
      "allele_count_reference_population": 41,
      "alphamissense_prediction": null,
      "alphamissense_score": null,
      "alt": "T",
      "apogee2_prediction": null,
      "apogee2_score": null,
      "bayesdelnoaf_prediction": null,
      "bayesdelnoaf_score": null,
      "chr": "5",
      "clinvar_classification": "Uncertain significance",
      "clinvar_disease": " early-onset,Frontotemporal dementia and/or amyotrophic lateral sclerosis 1,Frontotemporal dementia and/or amyotrophic lateral sclerosis 3,Paget disease of bone 2,not provided",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:3",
      "computational_prediction_selected": null,
      "computational_score_selected": null,
      "computational_source_selected": null,
      "consequences": [
        {
          "aa_alt": "N",
          "aa_end": null,
          "aa_length": 440,
          "aa_ref": "KN",
          "aa_start": 238,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2840,
          "cdna_start": 746,
          "cds_end": null,
          "cds_length": 1323,
          "cds_start": 714,
          "consequences": [
            "disruptive_inframe_deletion"
          ],
          "exon_count": 8,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "NM_003900.5",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.714_716delGAA",
          "hgvs_p": "p.Lys238del",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "ENST00000389805.9",
          "protein_coding": true,
          "protein_id": "NP_003891.1",
          "strand": true,
          "transcript": "NM_003900.5",
          "transcript_support_level": null
        },
        {
          "aa_alt": "N",
          "aa_end": null,
          "aa_length": 440,
          "aa_ref": "KN",
          "aa_start": 238,
          "biotype": "protein_coding",
          "canonical": true,
          "cdna_end": null,
          "cdna_length": 2840,
          "cdna_start": 746,
          "cds_end": null,
          "cds_length": 1323,
          "cds_start": 714,
          "consequences": [
            "disruptive_inframe_deletion"
          ],
          "exon_count": 8,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000389805.9",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.714_716delGAA",
          "hgvs_p": "p.Lys238del",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "NM_003900.5",
          "protein_coding": true,
          "protein_id": "ENSP00000374455.4",
          "strand": true,
          "transcript": "ENST00000389805.9",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "N",
          "aa_end": null,
          "aa_length": 356,
          "aa_ref": "KN",
          "aa_start": 154,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2253,
          "cdna_start": 982,
          "cds_end": null,
          "cds_length": 1071,
          "cds_start": 462,
          "consequences": [
            "disruptive_inframe_deletion"
          ],
          "exon_count": 7,
          "exon_rank": 4,
          "exon_rank_end": null,
          "feature": "ENST00000360718.5",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.462_464delGAA",
          "hgvs_p": "p.Lys154del",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000353944.5",
          "strand": true,
          "transcript": "ENST00000360718.5",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "N",
          "aa_end": null,
          "aa_length": 448,
          "aa_ref": "KN",
          "aa_start": 246,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2043,
          "cdna_start": 773,
          "cds_end": null,
          "cds_length": 1347,
          "cds_start": 738,
          "consequences": [
            "disruptive_inframe_deletion"
          ],
          "exon_count": 8,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000884700.1",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.738_740delGAA",
          "hgvs_p": "p.Lys246del",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000554759.1",
          "strand": true,
          "transcript": "ENST00000884700.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "N",
          "aa_end": null,
          "aa_length": 438,
          "aa_ref": "KN",
          "aa_start": 238,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2881,
          "cdna_start": 793,
          "cds_end": null,
          "cds_length": 1317,
          "cds_start": 714,
          "consequences": [
            "disruptive_inframe_deletion"
          ],
          "exon_count": 8,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000884698.1",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.714_716delGAA",
          "hgvs_p": "p.Lys238del",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000554757.1",
          "strand": true,
          "transcript": "ENST00000884698.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "N",
          "aa_end": null,
          "aa_length": 437,
          "aa_ref": "KN",
          "aa_start": 235,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2001,
          "cdna_start": 735,
          "cds_end": null,
          "cds_length": 1314,
          "cds_start": 705,
          "consequences": [
            "disruptive_inframe_deletion"
          ],
          "exon_count": 8,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000951526.1",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.705_707delGAA",
          "hgvs_p": "p.Lys235del",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000621585.1",
          "strand": true,
          "transcript": "ENST00000951526.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "N",
          "aa_end": null,
          "aa_length": 402,
          "aa_ref": "KN",
          "aa_start": 238,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1904,
          "cdna_start": 749,
          "cds_end": null,
          "cds_length": 1209,
          "cds_start": 714,
          "consequences": [
            "disruptive_inframe_deletion"
          ],
          "exon_count": 8,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000884703.1",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.714_716delGAA",
          "hgvs_p": "p.Lys238del",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000554762.1",
          "strand": true,
          "transcript": "ENST00000884703.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "N",
          "aa_end": null,
          "aa_length": 378,
          "aa_ref": "KN",
          "aa_start": 238,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1714,
          "cdna_start": 730,
          "cds_end": null,
          "cds_length": 1137,
          "cds_start": 714,
          "consequences": [
            "disruptive_inframe_deletion"
          ],
          "exon_count": 7,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000510187.5",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.714_716delGAA",
          "hgvs_p": "p.Lys238del",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000424477.1",
          "strand": true,
          "transcript": "ENST00000510187.5",
          "transcript_support_level": 5
        },
        {
          "aa_alt": "N",
          "aa_end": null,
          "aa_length": 356,
          "aa_ref": "KN",
          "aa_start": 154,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2911,
          "cdna_start": 817,
          "cds_end": null,
          "cds_length": 1071,
          "cds_start": 462,
          "consequences": [
            "disruptive_inframe_deletion"
          ],
          "exon_count": 9,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "NM_001142298.2",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.462_464delGAA",
          "hgvs_p": "p.Lys154del",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001135770.1",
          "strand": true,
          "transcript": "NM_001142298.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "N",
          "aa_end": null,
          "aa_length": 356,
          "aa_ref": "KN",
          "aa_start": 154,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2827,
          "cdna_start": 733,
          "cds_end": null,
          "cds_length": 1071,
          "cds_start": 462,
          "consequences": [
            "disruptive_inframe_deletion"
          ],
          "exon_count": 9,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "NM_001142299.2",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.462_464delGAA",
          "hgvs_p": "p.Lys154del",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001135771.1",
          "strand": true,
          "transcript": "NM_001142299.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "N",
          "aa_end": null,
          "aa_length": 303,
          "aa_ref": "KN",
          "aa_start": 238,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1605,
          "cdna_start": 748,
          "cds_end": null,
          "cds_length": 912,
          "cds_start": 714,
          "consequences": [
            "disruptive_inframe_deletion"
          ],
          "exon_count": 6,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000884704.1",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.714_716delGAA",
          "hgvs_p": "p.Lys238del",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000554763.1",
          "strand": true,
          "transcript": "ENST00000884704.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "N",
          "aa_end": null,
          "aa_length": 166,
          "aa_ref": "KN",
          "aa_start": 154,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 842,
          "cdna_start": 802,
          "cds_end": null,
          "cds_length": 502,
          "cds_start": 462,
          "consequences": [
            "disruptive_inframe_deletion"
          ],
          "exon_count": 6,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "ENST00000514093.5",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.462_464delGAA",
          "hgvs_p": "p.Lys154del",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000427308.1",
          "strand": true,
          "transcript": "ENST00000514093.5",
          "transcript_support_level": 5
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 413,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1938,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 1242,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 7,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000884702.1",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.673+863_673+865delGAA",
          "hgvs_p": null,
          "intron_rank": 4,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000554761.1",
          "strand": true,
          "transcript": "ENST00000884702.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 289,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2390,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 870,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 5,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000884699.1",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.301+2133_301+2135delGAA",
          "hgvs_p": null,
          "intron_rank": 2,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000554758.1",
          "strand": true,
          "transcript": "ENST00000884699.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 257,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1470,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 774,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 4,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000884701.1",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.205+4045_205+4047delGAA",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000554760.1",
          "strand": true,
          "transcript": "ENST00000884701.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 255,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1455,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 768,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 4,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000951527.1",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.205+4045_205+4047delGAA",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000621586.1",
          "strand": true,
          "transcript": "ENST00000951527.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 120,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1051,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 363,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 2,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000884705.1",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "c.205+4045_205+4047delGAA",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000554764.1",
          "strand": true,
          "transcript": "ENST00000884705.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "retained_intron",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 808,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 4,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000466342.1",
          "gene_hgnc_id": 11280,
          "gene_symbol": "SQSTM1",
          "hgvs_c": "n.413_415delGAA",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "ENST00000466342.1",
          "transcript_support_level": 2
        }
      ],
      "custom_annotations": null,
      "dbscsnv_ada_prediction": null,
      "dbscsnv_ada_score": null,
      "dbsnp": "rs796052214",
      "effect": "disruptive_inframe_deletion",
      "frequency_reference_population": 0.000025402254,
      "gene_hgnc_id": 11280,
      "gene_symbol": "SQSTM1",
      "gnomad_exomes_ac": 39,
      "gnomad_exomes_af": 0.0000266797,
      "gnomad_exomes_homalt": 1,
      "gnomad_genomes_ac": 2,
      "gnomad_genomes_af": 0.0000131366,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_heteroplasmic": null,
      "gnomad_mito_homoplasmic": null,
      "hom_count_reference_population": 1,
      "mitotip_prediction": null,
      "mitotip_score": null,
      "pathogenicity_classification_combined": "Uncertain significance",
      "phenotype_combined": "Frontotemporal dementia and/or amyotrophic lateral sclerosis 3|Paget disease of bone 2, early-onset;Frontotemporal dementia and/or amyotrophic lateral sclerosis 1|not provided",
      "phylop100way_prediction": "Uncertain_significance",
      "phylop100way_score": 6.408,
      "pos": 179825182,
      "ref": "TGAA",
      "revel_prediction": null,
      "revel_score": null,
      "splice_prediction_selected": "Benign",
      "splice_score_selected": 0.05999999865889549,
      "splice_source_selected": "max_spliceai",
      "spliceai_max_prediction": "Benign",
      "spliceai_max_score": 0.06,
      "transcript": "NM_003900.5"
    }
  ]
}
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