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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-251353-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=251353&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 251353,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_004168.4",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1679C>T",
          "hgvs_p": "p.Thr560Met",
          "transcript": "NM_004168.4",
          "protein_id": "NP_004159.2",
          "transcript_support_level": null,
          "aa_start": 560,
          "aa_end": null,
          "aa_length": 664,
          "cds_start": 1679,
          "cds_end": null,
          "cds_length": 1995,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000264932.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004168.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1679C>T",
          "hgvs_p": "p.Thr560Met",
          "transcript": "ENST00000264932.11",
          "protein_id": "ENSP00000264932.6",
          "transcript_support_level": 1,
          "aa_start": 560,
          "aa_end": null,
          "aa_length": 664,
          "cds_start": 1679,
          "cds_end": null,
          "cds_length": 1995,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_004168.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000264932.11"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000286001",
          "gene_hgnc_id": null,
          "hgvs_c": "n.*412C>T",
          "hgvs_p": null,
          "transcript": "ENST00000651543.1",
          "protein_id": "ENSP00000499215.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000651543.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000286001",
          "gene_hgnc_id": null,
          "hgvs_c": "n.*412C>T",
          "hgvs_p": null,
          "transcript": "ENST00000651543.1",
          "protein_id": "ENSP00000499215.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000651543.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1865C>T",
          "hgvs_p": "p.Thr622Met",
          "transcript": "ENST00000874235.1",
          "protein_id": "ENSP00000544294.1",
          "transcript_support_level": null,
          "aa_start": 622,
          "aa_end": null,
          "aa_length": 726,
          "cds_start": 1865,
          "cds_end": null,
          "cds_length": 2181,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874235.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1832C>T",
          "hgvs_p": "p.Thr611Met",
          "transcript": "ENST00000952715.1",
          "protein_id": "ENSP00000622774.1",
          "transcript_support_level": null,
          "aa_start": 611,
          "aa_end": null,
          "aa_length": 715,
          "cds_start": 1832,
          "cds_end": null,
          "cds_length": 2148,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952715.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1733C>T",
          "hgvs_p": "p.Thr578Met",
          "transcript": "ENST00000874242.1",
          "protein_id": "ENSP00000544301.1",
          "transcript_support_level": null,
          "aa_start": 578,
          "aa_end": null,
          "aa_length": 682,
          "cds_start": 1733,
          "cds_end": null,
          "cds_length": 2049,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874242.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1730C>T",
          "hgvs_p": "p.Thr577Met",
          "transcript": "ENST00000952713.1",
          "protein_id": "ENSP00000622772.1",
          "transcript_support_level": null,
          "aa_start": 577,
          "aa_end": null,
          "aa_length": 681,
          "cds_start": 1730,
          "cds_end": null,
          "cds_length": 2046,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952713.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1706C>T",
          "hgvs_p": "p.Thr569Met",
          "transcript": "ENST00000952712.1",
          "protein_id": "ENSP00000622771.1",
          "transcript_support_level": null,
          "aa_start": 569,
          "aa_end": null,
          "aa_length": 673,
          "cds_start": 1706,
          "cds_end": null,
          "cds_length": 2022,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952712.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1703C>T",
          "hgvs_p": "p.Thr568Met",
          "transcript": "ENST00000874248.1",
          "protein_id": "ENSP00000544307.1",
          "transcript_support_level": null,
          "aa_start": 568,
          "aa_end": null,
          "aa_length": 672,
          "cds_start": 1703,
          "cds_end": null,
          "cds_length": 2019,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874248.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1685C>T",
          "hgvs_p": "p.Thr562Met",
          "transcript": "ENST00000952708.1",
          "protein_id": "ENSP00000622767.1",
          "transcript_support_level": null,
          "aa_start": 562,
          "aa_end": null,
          "aa_length": 666,
          "cds_start": 1685,
          "cds_end": null,
          "cds_length": 2001,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952708.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1676C>T",
          "hgvs_p": "p.Thr559Met",
          "transcript": "ENST00000874234.1",
          "protein_id": "ENSP00000544293.1",
          "transcript_support_level": null,
          "aa_start": 559,
          "aa_end": null,
          "aa_length": 663,
          "cds_start": 1676,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874234.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1676C>T",
          "hgvs_p": "p.Thr559Met",
          "transcript": "ENST00000952707.1",
          "protein_id": "ENSP00000622766.1",
          "transcript_support_level": null,
          "aa_start": 559,
          "aa_end": null,
          "aa_length": 663,
          "cds_start": 1676,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952707.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1655C>T",
          "hgvs_p": "p.Thr552Met",
          "transcript": "ENST00000874246.1",
          "protein_id": "ENSP00000544305.1",
          "transcript_support_level": null,
          "aa_start": 552,
          "aa_end": null,
          "aa_length": 656,
          "cds_start": 1655,
          "cds_end": null,
          "cds_length": 1971,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874246.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1646C>T",
          "hgvs_p": "p.Thr549Met",
          "transcript": "ENST00000874244.1",
          "protein_id": "ENSP00000544303.1",
          "transcript_support_level": null,
          "aa_start": 549,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": 1646,
          "cds_end": null,
          "cds_length": 1962,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874244.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1646C>T",
          "hgvs_p": "p.Thr549Met",
          "transcript": "ENST00000952709.1",
          "protein_id": "ENSP00000622768.1",
          "transcript_support_level": null,
          "aa_start": 549,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": 1646,
          "cds_end": null,
          "cds_length": 1962,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952709.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1634C>T",
          "hgvs_p": "p.Thr545Met",
          "transcript": "ENST00000952716.1",
          "protein_id": "ENSP00000622775.1",
          "transcript_support_level": null,
          "aa_start": 545,
          "aa_end": null,
          "aa_length": 649,
          "cds_start": 1634,
          "cds_end": null,
          "cds_length": 1950,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952716.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1628C>T",
          "hgvs_p": "p.Thr543Met",
          "transcript": "ENST00000952711.1",
          "protein_id": "ENSP00000622770.1",
          "transcript_support_level": null,
          "aa_start": 543,
          "aa_end": null,
          "aa_length": 647,
          "cds_start": 1628,
          "cds_end": null,
          "cds_length": 1944,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952711.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1679C>T",
          "hgvs_p": "p.Thr560Met",
          "transcript": "ENST00000874236.1",
          "protein_id": "ENSP00000544295.1",
          "transcript_support_level": null,
          "aa_start": 560,
          "aa_end": null,
          "aa_length": 626,
          "cds_start": 1679,
          "cds_end": null,
          "cds_length": 1881,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874236.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1535C>T",
          "hgvs_p": "p.Thr512Met",
          "transcript": "NM_001294332.2",
          "protein_id": "NP_001281261.1",
          "transcript_support_level": null,
          "aa_start": 512,
          "aa_end": null,
          "aa_length": 616,
          "cds_start": 1535,
          "cds_end": null,
          "cds_length": 1851,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
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      "computational_source_selected": "MetaRNN",
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      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Pathogenic",
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      "bayesdelnoaf_score": 0.21,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.543,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM1,PP3_Moderate",
      "acmg_by_gene": [
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          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
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            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_004168.4",
          "gene_symbol": "SDHA",
          "hgnc_id": 10680,
          "effects": [
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          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.1679C>T",
          "hgvs_p": "p.Thr560Met"
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        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
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            "PP3_Moderate"
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          "verdict": "Uncertain_significance",
          "transcript": "ENST00000651543.1",
          "gene_symbol": "ENSG00000286001",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
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          "inheritance_mode": "",
          "hgvs_c": "n.*412C>T",
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      ],
      "clinvar_disease": " nuclear type 1,Dilated cardiomyopathy 1GG,Hereditary cancer-predisposing syndrome,Mitochondrial complex II deficiency,Neurodegeneration with ataxia and late-onset optic atrophy,Pheochromocytoma/paraganglioma syndrome 5,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:5 O:1",
      "phenotype_combined": "Mitochondrial complex II deficiency, nuclear type 1;Pheochromocytoma/paraganglioma syndrome 5|Hereditary cancer-predisposing syndrome|Dilated cardiomyopathy 1GG|not provided|Mitochondrial complex II deficiency, nuclear type 1;Neurodegeneration with ataxia and late-onset optic atrophy;Pheochromocytoma/paraganglioma syndrome 5;Dilated cardiomyopathy 1GG",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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