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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-256376-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=256376&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 256376,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_004168.4",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1951G>C",
          "hgvs_p": "p.Glu651Gln",
          "transcript": "NM_004168.4",
          "protein_id": "NP_004159.2",
          "transcript_support_level": null,
          "aa_start": 651,
          "aa_end": null,
          "aa_length": 664,
          "cds_start": 1951,
          "cds_end": null,
          "cds_length": 1995,
          "cdna_start": 1987,
          "cdna_end": null,
          "cdna_length": 2693,
          "mane_select": "ENST00000264932.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004168.4"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1951G>C",
          "hgvs_p": "p.Glu651Gln",
          "transcript": "ENST00000264932.11",
          "protein_id": "ENSP00000264932.6",
          "transcript_support_level": 1,
          "aa_start": 651,
          "aa_end": null,
          "aa_length": 664,
          "cds_start": 1951,
          "cds_end": null,
          "cds_length": 1995,
          "cdna_start": 1987,
          "cdna_end": null,
          "cdna_length": 2693,
          "mane_select": "NM_004168.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000264932.11"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000286001",
          "gene_hgnc_id": null,
          "hgvs_c": "n.*684G>C",
          "hgvs_p": null,
          "transcript": "ENST00000651543.1",
          "protein_id": "ENSP00000499215.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3665,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000651543.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000286001",
          "gene_hgnc_id": null,
          "hgvs_c": "n.*684G>C",
          "hgvs_p": null,
          "transcript": "ENST00000651543.1",
          "protein_id": "ENSP00000499215.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3665,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000651543.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.2137G>C",
          "hgvs_p": "p.Glu713Gln",
          "transcript": "ENST00000874235.1",
          "protein_id": "ENSP00000544294.1",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 726,
          "cds_start": 2137,
          "cds_end": null,
          "cds_length": 2181,
          "cdna_start": 2190,
          "cdna_end": null,
          "cdna_length": 2511,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874235.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.2104G>C",
          "hgvs_p": "p.Glu702Gln",
          "transcript": "ENST00000952715.1",
          "protein_id": "ENSP00000622774.1",
          "transcript_support_level": null,
          "aa_start": 702,
          "aa_end": null,
          "aa_length": 715,
          "cds_start": 2104,
          "cds_end": null,
          "cds_length": 2148,
          "cdna_start": 2133,
          "cdna_end": null,
          "cdna_length": 2417,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952715.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.2005G>C",
          "hgvs_p": "p.Glu669Gln",
          "transcript": "ENST00000874242.1",
          "protein_id": "ENSP00000544301.1",
          "transcript_support_level": null,
          "aa_start": 669,
          "aa_end": null,
          "aa_length": 682,
          "cds_start": 2005,
          "cds_end": null,
          "cds_length": 2049,
          "cdna_start": 2049,
          "cdna_end": null,
          "cdna_length": 2355,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874242.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.2002G>C",
          "hgvs_p": "p.Glu668Gln",
          "transcript": "ENST00000952713.1",
          "protein_id": "ENSP00000622772.1",
          "transcript_support_level": null,
          "aa_start": 668,
          "aa_end": null,
          "aa_length": 681,
          "cds_start": 2002,
          "cds_end": null,
          "cds_length": 2046,
          "cdna_start": 2038,
          "cdna_end": null,
          "cdna_length": 2322,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952713.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1978G>C",
          "hgvs_p": "p.Glu660Gln",
          "transcript": "ENST00000952712.1",
          "protein_id": "ENSP00000622771.1",
          "transcript_support_level": null,
          "aa_start": 660,
          "aa_end": null,
          "aa_length": 673,
          "cds_start": 1978,
          "cds_end": null,
          "cds_length": 2022,
          "cdna_start": 2025,
          "cdna_end": null,
          "cdna_length": 2306,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952712.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1975G>C",
          "hgvs_p": "p.Glu659Gln",
          "transcript": "ENST00000874248.1",
          "protein_id": "ENSP00000544307.1",
          "transcript_support_level": null,
          "aa_start": 659,
          "aa_end": null,
          "aa_length": 672,
          "cds_start": 1975,
          "cds_end": null,
          "cds_length": 2019,
          "cdna_start": 1978,
          "cdna_end": null,
          "cdna_length": 2285,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874248.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1957G>C",
          "hgvs_p": "p.Glu653Gln",
          "transcript": "ENST00000952708.1",
          "protein_id": "ENSP00000622767.1",
          "transcript_support_level": null,
          "aa_start": 653,
          "aa_end": null,
          "aa_length": 666,
          "cds_start": 1957,
          "cds_end": null,
          "cds_length": 2001,
          "cdna_start": 2032,
          "cdna_end": null,
          "cdna_length": 2333,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952708.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1948G>C",
          "hgvs_p": "p.Glu650Gln",
          "transcript": "ENST00000874234.1",
          "protein_id": "ENSP00000544293.1",
          "transcript_support_level": null,
          "aa_start": 650,
          "aa_end": null,
          "aa_length": 663,
          "cds_start": 1948,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": 2001,
          "cdna_end": null,
          "cdna_length": 2324,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874234.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1948G>C",
          "hgvs_p": "p.Glu650Gln",
          "transcript": "ENST00000952707.1",
          "protein_id": "ENSP00000622766.1",
          "transcript_support_level": null,
          "aa_start": 650,
          "aa_end": null,
          "aa_length": 663,
          "cds_start": 1948,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": 2030,
          "cdna_end": null,
          "cdna_length": 2737,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952707.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1927G>C",
          "hgvs_p": "p.Glu643Gln",
          "transcript": "ENST00000874246.1",
          "protein_id": "ENSP00000544305.1",
          "transcript_support_level": null,
          "aa_start": 643,
          "aa_end": null,
          "aa_length": 656,
          "cds_start": 1927,
          "cds_end": null,
          "cds_length": 1971,
          "cdna_start": 1962,
          "cdna_end": null,
          "cdna_length": 2260,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874246.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1918G>C",
          "hgvs_p": "p.Glu640Gln",
          "transcript": "ENST00000874244.1",
          "protein_id": "ENSP00000544303.1",
          "transcript_support_level": null,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": 1918,
          "cds_end": null,
          "cds_length": 1962,
          "cdna_start": 1953,
          "cdna_end": null,
          "cdna_length": 2259,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874244.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1918G>C",
          "hgvs_p": "p.Glu640Gln",
          "transcript": "ENST00000952709.1",
          "protein_id": "ENSP00000622768.1",
          "transcript_support_level": null,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": 1918,
          "cds_end": null,
          "cds_length": 1962,
          "cdna_start": 1963,
          "cdna_end": null,
          "cdna_length": 2269,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952709.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1906G>C",
          "hgvs_p": "p.Glu636Gln",
          "transcript": "ENST00000952716.1",
          "protein_id": "ENSP00000622775.1",
          "transcript_support_level": null,
          "aa_start": 636,
          "aa_end": null,
          "aa_length": 649,
          "cds_start": 1906,
          "cds_end": null,
          "cds_length": 1950,
          "cdna_start": 1931,
          "cdna_end": null,
          "cdna_length": 2215,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952716.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1900G>C",
          "hgvs_p": "p.Glu634Gln",
          "transcript": "ENST00000952711.1",
          "protein_id": "ENSP00000622770.1",
          "transcript_support_level": null,
          "aa_start": 634,
          "aa_end": null,
          "aa_length": 647,
          "cds_start": 1900,
          "cds_end": null,
          "cds_length": 1944,
          "cdna_start": 1929,
          "cdna_end": null,
          "cdna_length": 2238,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952711.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1837G>C",
          "hgvs_p": "p.Glu613Gln",
          "transcript": "ENST00000874236.1",
          "protein_id": "ENSP00000544295.1",
          "transcript_support_level": null,
          "aa_start": 613,
          "aa_end": null,
          "aa_length": 626,
          "cds_start": 1837,
          "cds_end": null,
          "cds_length": 1881,
          "cdna_start": 1888,
          "cdna_end": null,
          "cdna_length": 2194,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874236.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1807G>C",
          "hgvs_p": "p.Glu603Gln",
          "transcript": "NM_001294332.2",
          "protein_id": "NP_001281261.1",
          "transcript_support_level": null,
          "aa_start": 603,
          "aa_end": null,
          "aa_length": 616,
          "cds_start": 1807,
          "cds_end": null,
          "cds_length": 1851,
          "cdna_start": 1843,
          "cdna_end": null,
          "cdna_length": 2549,
          "mane_select": null,
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      "computational_score_selected": 0.3352581560611725,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
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      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Uncertain_significance",
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      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.16,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.397,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
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      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
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      "acmg_by_gene": [
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          "verdict": "Uncertain_significance",
          "transcript": "NM_004168.4",
          "gene_symbol": "SDHA",
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        {
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          "verdict": "Uncertain_significance",
          "transcript": "ENST00000651543.1",
          "gene_symbol": "ENSG00000286001",
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          "effects": [
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          "inheritance_mode": "",
          "hgvs_c": "n.*684G>C",
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      ],
      "clinvar_disease": " nuclear type 1,Hereditary cancer-predisposing syndrome,Mitochondrial complex II deficiency,Pheochromocytoma/paraganglioma syndrome 5",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:3",
      "phenotype_combined": "Pheochromocytoma/paraganglioma syndrome 5|Mitochondrial complex II deficiency, nuclear type 1;Pheochromocytoma/paraganglioma syndrome 5|Hereditary cancer-predisposing syndrome",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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