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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-34807851-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=34807851&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 34807851,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001145525.2",
      "consequences": [
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "NM_015577.3",
          "protein_id": "NP_056392.2",
          "transcript_support_level": null,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 980,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 2943,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000265109.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_015577.3"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "ENST00000265109.8",
          "protein_id": "ENSP00000265109.3",
          "transcript_support_level": 1,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 980,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 2943,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_015577.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000265109.8"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.382T>C",
          "hgvs_p": "p.Tyr128His",
          "transcript": "ENST00000515799.5",
          "protein_id": "ENSP00000427123.1",
          "transcript_support_level": 1,
          "aa_start": 128,
          "aa_end": null,
          "aa_length": 983,
          "cds_start": 382,
          "cds_end": null,
          "cds_length": 2952,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000515799.5"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "ENST00000428746.6",
          "protein_id": "ENSP00000388725.2",
          "transcript_support_level": 1,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 980,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 2943,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000428746.6"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.349T>C",
          "hgvs_p": "p.Tyr117His",
          "transcript": "ENST00000506376.1",
          "protein_id": "ENSP00000423854.1",
          "transcript_support_level": 1,
          "aa_start": 117,
          "aa_end": null,
          "aa_length": 972,
          "cds_start": 349,
          "cds_end": null,
          "cds_length": 2919,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000506376.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "ENST00000512629.5",
          "protein_id": "ENSP00000422377.1",
          "transcript_support_level": 1,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 951,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 2856,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000512629.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "n.*156T>C",
          "hgvs_p": null,
          "transcript": "ENST00000508777.5",
          "protein_id": "ENSP00000423965.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000508777.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "n.*156T>C",
          "hgvs_p": null,
          "transcript": "ENST00000508777.5",
          "protein_id": "ENSP00000423965.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000508777.5"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.382T>C",
          "hgvs_p": "p.Tyr128His",
          "transcript": "NM_001145525.2",
          "protein_id": "NP_001138997.1",
          "transcript_support_level": null,
          "aa_start": 128,
          "aa_end": null,
          "aa_length": 983,
          "cds_start": 382,
          "cds_end": null,
          "cds_length": 2952,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145525.2"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "NM_001145520.1",
          "protein_id": "NP_001138992.1",
          "transcript_support_level": null,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 980,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 2943,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145520.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "NM_001145521.2",
          "protein_id": "NP_001138993.1",
          "transcript_support_level": null,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 980,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 2943,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145521.2"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "ENST00000503673.5",
          "protein_id": "ENSP00000422942.1",
          "transcript_support_level": 2,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 980,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 2943,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000503673.5"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "ENST00000957306.1",
          "protein_id": "ENSP00000627365.1",
          "transcript_support_level": null,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 980,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 2943,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957306.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.349T>C",
          "hgvs_p": "p.Tyr117His",
          "transcript": "NM_001145523.2",
          "protein_id": "NP_001138995.1",
          "transcript_support_level": null,
          "aa_start": 117,
          "aa_end": null,
          "aa_length": 972,
          "cds_start": 349,
          "cds_end": null,
          "cds_length": 2919,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145523.2"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "NM_001145522.2",
          "protein_id": "NP_001138994.1",
          "transcript_support_level": null,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 951,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 2856,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145522.2"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "ENST00000902575.1",
          "protein_id": "ENSP00000572634.1",
          "transcript_support_level": null,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 951,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 2856,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902575.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "ENST00000902576.1",
          "protein_id": "ENSP00000572635.1",
          "transcript_support_level": null,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 951,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 2856,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902576.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "ENST00000957307.1",
          "protein_id": "ENSP00000627366.1",
          "transcript_support_level": null,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 951,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 2856,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957307.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "ENST00000957308.1",
          "protein_id": "ENSP00000627367.1",
          "transcript_support_level": null,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 951,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 2856,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957308.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAI14",
          "gene_hgnc_id": 14873,
          "hgvs_c": "c.373T>C",
          "hgvs_p": "p.Tyr125His",
          "transcript": "ENST00000512625.5",
          "protein_id": "ENSP00000425115.1",
          "transcript_support_level": 4,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 149,
          "cds_start": 373,
          "cds_end": null,
          "cds_length": 450,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000512625.5"
        },
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        {
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          "biotype": "nonsense_mediated_decay",
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        },
        {
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          "protein_coding": false,
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          "consequences": [
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          "hgvs_c": "n.*218T>C",
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          "protein_id": "ENSP00000424507.1",
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          "biotype": "nonsense_mediated_decay",
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        },
        {
          "aa_ref": null,
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          "protein_coding": true,
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          "consequences": [
            "downstream_gene_variant"
          ],
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          "exon_count": 6,
          "intron_rank": null,
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          "gene_symbol": "RAI14",
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          "hgvs_c": "c.*31T>C",
          "hgvs_p": null,
          "transcript": "ENST00000504052.5",
          "protein_id": "ENSP00000422515.1",
          "transcript_support_level": 4,
          "aa_start": null,
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          "cds_start": null,
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          "cds_length": 342,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000504052.5"
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      ],
      "gene_symbol": "RAI14",
      "gene_hgnc_id": 14873,
      "dbsnp": "rs754745101",
      "frequency_reference_population": 0.00011609657,
      "hom_count_reference_population": 2,
      "allele_count_reference_population": 187,
      "gnomad_exomes_af": 0.000125481,
      "gnomad_genomes_af": 0.0000262571,
      "gnomad_exomes_ac": 183,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 2,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.24194005131721497,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.248,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.186,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.23,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.559,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -6,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate,BS2",
      "acmg_by_gene": [
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001145525.2",
          "gene_symbol": "RAI14",
          "hgnc_id": 14873,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.382T>C",
          "hgvs_p": "p.Tyr128His"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}