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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-34998807-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=34998807&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 34998807,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_031900.4",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1457C>T",
          "hgvs_p": "p.Ser486Leu",
          "transcript": "NM_031900.4",
          "protein_id": "NP_114106.1",
          "transcript_support_level": null,
          "aa_start": 486,
          "aa_end": null,
          "aa_length": 514,
          "cds_start": 1457,
          "cds_end": null,
          "cds_length": 1545,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000231420.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_031900.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1457C>T",
          "hgvs_p": "p.Ser486Leu",
          "transcript": "ENST00000231420.11",
          "protein_id": "ENSP00000231420.6",
          "transcript_support_level": 1,
          "aa_start": 486,
          "aa_end": null,
          "aa_length": 514,
          "cds_start": 1457,
          "cds_end": null,
          "cds_length": 1545,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_031900.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000231420.11"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1232C>T",
          "hgvs_p": "p.Ser411Leu",
          "transcript": "ENST00000510428.1",
          "protein_id": "ENSP00000422799.1",
          "transcript_support_level": 1,
          "aa_start": 411,
          "aa_end": null,
          "aa_length": 439,
          "cds_start": 1232,
          "cds_end": null,
          "cds_length": 1320,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000510428.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1538C>T",
          "hgvs_p": "p.Ser513Leu",
          "transcript": "ENST00000853198.1",
          "protein_id": "ENSP00000523257.1",
          "transcript_support_level": null,
          "aa_start": 513,
          "aa_end": null,
          "aa_length": 541,
          "cds_start": 1538,
          "cds_end": null,
          "cds_length": 1626,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853198.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1535C>T",
          "hgvs_p": "p.Ser512Leu",
          "transcript": "ENST00000853200.1",
          "protein_id": "ENSP00000523259.1",
          "transcript_support_level": null,
          "aa_start": 512,
          "aa_end": null,
          "aa_length": 540,
          "cds_start": 1535,
          "cds_end": null,
          "cds_length": 1623,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853200.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1517C>T",
          "hgvs_p": "p.Ser506Leu",
          "transcript": "ENST00000853201.1",
          "protein_id": "ENSP00000523260.1",
          "transcript_support_level": null,
          "aa_start": 506,
          "aa_end": null,
          "aa_length": 534,
          "cds_start": 1517,
          "cds_end": null,
          "cds_length": 1605,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853201.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1454C>T",
          "hgvs_p": "p.Ser485Leu",
          "transcript": "NM_001438583.1",
          "protein_id": "NP_001425512.1",
          "transcript_support_level": null,
          "aa_start": 485,
          "aa_end": null,
          "aa_length": 513,
          "cds_start": 1454,
          "cds_end": null,
          "cds_length": 1542,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438583.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1454C>T",
          "hgvs_p": "p.Ser485Leu",
          "transcript": "ENST00000853195.1",
          "protein_id": "ENSP00000523254.1",
          "transcript_support_level": null,
          "aa_start": 485,
          "aa_end": null,
          "aa_length": 513,
          "cds_start": 1454,
          "cds_end": null,
          "cds_length": 1542,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853195.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1307C>T",
          "hgvs_p": "p.Ser436Leu",
          "transcript": "ENST00000853199.1",
          "protein_id": "ENSP00000523258.1",
          "transcript_support_level": null,
          "aa_start": 436,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 1307,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853199.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1262C>T",
          "hgvs_p": "p.Ser421Leu",
          "transcript": "NM_001438584.1",
          "protein_id": "NP_001425513.1",
          "transcript_support_level": null,
          "aa_start": 421,
          "aa_end": null,
          "aa_length": 449,
          "cds_start": 1262,
          "cds_end": null,
          "cds_length": 1350,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438584.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1262C>T",
          "hgvs_p": "p.Ser421Leu",
          "transcript": "ENST00000853196.1",
          "protein_id": "ENSP00000523255.1",
          "transcript_support_level": null,
          "aa_start": 421,
          "aa_end": null,
          "aa_length": 449,
          "cds_start": 1262,
          "cds_end": null,
          "cds_length": 1350,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853196.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1259C>T",
          "hgvs_p": "p.Ser420Leu",
          "transcript": "ENST00000853197.1",
          "protein_id": "ENSP00000523256.1",
          "transcript_support_level": null,
          "aa_start": 420,
          "aa_end": null,
          "aa_length": 448,
          "cds_start": 1259,
          "cds_end": null,
          "cds_length": 1347,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853197.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1232C>T",
          "hgvs_p": "p.Ser411Leu",
          "transcript": "NM_001306173.2",
          "protein_id": "NP_001293102.1",
          "transcript_support_level": null,
          "aa_start": 411,
          "aa_end": null,
          "aa_length": 439,
          "cds_start": 1232,
          "cds_end": null,
          "cds_length": 1320,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001306173.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1232C>T",
          "hgvs_p": "p.Ser411Leu",
          "transcript": "NM_001438050.1",
          "protein_id": "NP_001424979.1",
          "transcript_support_level": null,
          "aa_start": 411,
          "aa_end": null,
          "aa_length": 439,
          "cds_start": 1232,
          "cds_end": null,
          "cds_length": 1320,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438050.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1232C>T",
          "hgvs_p": "p.Ser411Leu",
          "transcript": "ENST00000618015.4",
          "protein_id": "ENSP00000479154.1",
          "transcript_support_level": 5,
          "aa_start": 411,
          "aa_end": null,
          "aa_length": 439,
          "cds_start": 1232,
          "cds_end": null,
          "cds_length": 1320,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000618015.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1181C>T",
          "hgvs_p": "p.Ser394Leu",
          "transcript": "NM_001438585.1",
          "protein_id": "NP_001425514.1",
          "transcript_support_level": null,
          "aa_start": 394,
          "aa_end": null,
          "aa_length": 422,
          "cds_start": 1181,
          "cds_end": null,
          "cds_length": 1269,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438585.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1148C>T",
          "hgvs_p": "p.Ser383Leu",
          "transcript": "ENST00000946517.1",
          "protein_id": "ENSP00000616576.1",
          "transcript_support_level": null,
          "aa_start": 383,
          "aa_end": null,
          "aa_length": 411,
          "cds_start": 1148,
          "cds_end": null,
          "cds_length": 1236,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946517.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "c.1082C>T",
          "hgvs_p": "p.Ser361Leu",
          "transcript": "ENST00000853202.1",
          "protein_id": "ENSP00000523261.1",
          "transcript_support_level": null,
          "aa_start": 361,
          "aa_end": null,
          "aa_length": 389,
          "cds_start": 1082,
          "cds_end": null,
          "cds_length": 1170,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853202.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGXT2",
          "gene_hgnc_id": 14412,
          "hgvs_c": "n.1127C>T",
          "hgvs_p": null,
          "transcript": "ENST00000512135.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000512135.5"
        }
      ],
      "gene_symbol": "AGXT2",
      "gene_hgnc_id": 14412,
      "dbsnp": "rs529653742",
      "frequency_reference_population": 0.000022309643,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 36,
      "gnomad_exomes_af": 0.0000082116,
      "gnomad_genomes_af": 0.00015758,
      "gnomad_exomes_ac": 12,
      "gnomad_genomes_ac": 24,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.26019275188446045,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.216,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2568,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.31,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.713,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_031900.4",
          "gene_symbol": "AGXT2",
          "hgnc_id": 14412,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1457C>T",
          "hgvs_p": "p.Ser486Leu"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}