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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-40832523-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=40832523&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 40832523,
      "ref": "G",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_000997.5",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL37",
          "gene_hgnc_id": 10347,
          "hgvs_c": "c.275C>A",
          "hgvs_p": "p.Ala92Glu",
          "transcript": "NM_000997.5",
          "protein_id": "NP_000988.1",
          "transcript_support_level": null,
          "aa_start": 92,
          "aa_end": null,
          "aa_length": 97,
          "cds_start": 275,
          "cds_end": null,
          "cds_length": 294,
          "cdna_start": 312,
          "cdna_end": null,
          "cdna_length": 7573,
          "mane_select": "ENST00000274242.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000997.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "E",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL37",
          "gene_hgnc_id": 10347,
          "hgvs_c": "c.275C>A",
          "hgvs_p": "p.Ala92Glu",
          "transcript": "ENST00000274242.10",
          "protein_id": "ENSP00000274242.5",
          "transcript_support_level": 1,
          "aa_start": 92,
          "aa_end": null,
          "aa_length": 97,
          "cds_start": 275,
          "cds_end": null,
          "cds_length": 294,
          "cdna_start": 312,
          "cdna_end": null,
          "cdna_length": 7573,
          "mane_select": "NM_000997.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000274242.10"
        },
        {
          "aa_ref": "A",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL37",
          "gene_hgnc_id": 10347,
          "hgvs_c": "c.356C>A",
          "hgvs_p": "p.Ala119Glu",
          "transcript": "ENST00000916328.1",
          "protein_id": "ENSP00000586387.1",
          "transcript_support_level": null,
          "aa_start": 119,
          "aa_end": null,
          "aa_length": 124,
          "cds_start": 356,
          "cds_end": null,
          "cds_length": 375,
          "cdna_start": 386,
          "cdna_end": null,
          "cdna_length": 453,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916328.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL37",
          "gene_hgnc_id": 10347,
          "hgvs_c": "c.332C>A",
          "hgvs_p": "p.Ala111Glu",
          "transcript": "ENST00000916326.1",
          "protein_id": "ENSP00000586385.1",
          "transcript_support_level": null,
          "aa_start": 111,
          "aa_end": null,
          "aa_length": 116,
          "cds_start": 332,
          "cds_end": null,
          "cds_length": 351,
          "cdna_start": 385,
          "cdna_end": null,
          "cdna_length": 454,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916326.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL37",
          "gene_hgnc_id": 10347,
          "hgvs_c": "c.263C>A",
          "hgvs_p": "p.Ala88Glu",
          "transcript": "ENST00000916325.1",
          "protein_id": "ENSP00000586384.1",
          "transcript_support_level": null,
          "aa_start": 88,
          "aa_end": null,
          "aa_length": 93,
          "cds_start": 263,
          "cds_end": null,
          "cds_length": 282,
          "cdna_start": 347,
          "cdna_end": null,
          "cdna_length": 416,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916325.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL37",
          "gene_hgnc_id": 10347,
          "hgvs_c": "c.248C>A",
          "hgvs_p": "p.Ala83Glu",
          "transcript": "ENST00000916330.1",
          "protein_id": "ENSP00000586389.1",
          "transcript_support_level": null,
          "aa_start": 83,
          "aa_end": null,
          "aa_length": 88,
          "cds_start": 248,
          "cds_end": null,
          "cds_length": 267,
          "cdna_start": 275,
          "cdna_end": null,
          "cdna_length": 342,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916330.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL37",
          "gene_hgnc_id": 10347,
          "hgvs_c": "c.236C>A",
          "hgvs_p": "p.Ala79Glu",
          "transcript": "ENST00000916327.1",
          "protein_id": "ENSP00000586386.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 84,
          "cds_start": 236,
          "cds_end": null,
          "cds_length": 255,
          "cdna_start": 267,
          "cdna_end": null,
          "cdna_length": 336,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916327.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL37",
          "gene_hgnc_id": 10347,
          "hgvs_c": "c.190C>A",
          "hgvs_p": "p.Gln64Lys",
          "transcript": "ENST00000509877.5",
          "protein_id": "ENSP00000422823.1",
          "transcript_support_level": 5,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 74,
          "cds_start": 190,
          "cds_end": null,
          "cds_length": 225,
          "cdna_start": 217,
          "cdna_end": null,
          "cdna_length": 637,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000509877.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL37",
          "gene_hgnc_id": 10347,
          "hgvs_c": "c.188C>A",
          "hgvs_p": "p.Ala63Glu",
          "transcript": "ENST00000916329.1",
          "protein_id": "ENSP00000586388.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 68,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 207,
          "cdna_start": 215,
          "cdna_end": null,
          "cdna_length": 284,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916329.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL37",
          "gene_hgnc_id": 10347,
          "hgvs_c": "n.440C>A",
          "hgvs_p": null,
          "transcript": "ENST00000504562.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 505,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000504562.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL37",
          "gene_hgnc_id": 10347,
          "hgvs_c": "n.350C>A",
          "hgvs_p": null,
          "transcript": "ENST00000507642.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 425,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000507642.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL37",
          "gene_hgnc_id": 10347,
          "hgvs_c": "n.33C>A",
          "hgvs_p": null,
          "transcript": "ENST00000511787.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 435,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000511787.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL37",
          "gene_hgnc_id": 10347,
          "hgvs_c": "n.312C>A",
          "hgvs_p": null,
          "transcript": "NR_159993.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3083,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_159993.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SNORD72",
          "gene_hgnc_id": 32733,
          "hgvs_c": "n.*133C>A",
          "hgvs_p": null,
          "transcript": "ENST00000390994.1",
          "protein_id": null,
          "transcript_support_level": 6,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 80,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "snoRNA",
          "feature": "ENST00000390994.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SNORD72",
          "gene_hgnc_id": 32733,
          "hgvs_c": "n.*133C>A",
          "hgvs_p": null,
          "transcript": "NR_002583.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 80,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "snoRNA",
          "feature": "NR_002583.1"
        }
      ],
      "gene_symbol": "RPL37",
      "gene_hgnc_id": 10347,
      "dbsnp": "rs770111750",
      "frequency_reference_population": 0.000008676478,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 14,
      "gnomad_exomes_af": 0.00000821146,
      "gnomad_genomes_af": 0.0000131418,
      "gnomad_exomes_ac": 12,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.5335232019424438,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.328,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.5702,
      "alphamissense_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.15,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.661,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_000997.5",
          "gene_symbol": "RPL37",
          "hgnc_id": 10347,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.275C>A",
          "hgvs_p": "p.Ala92Glu"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NR_002583.1",
          "gene_symbol": "SNORD72",
          "hgnc_id": 32733,
          "effects": [
            "downstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.*133C>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}
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