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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-55259905-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=55259905&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 55259905,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_019030.4",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "c.4000G>A",
          "hgvs_p": "p.Val1334Ile",
          "transcript": "NM_019030.4",
          "protein_id": "NP_061903.2",
          "transcript_support_level": null,
          "aa_start": 1334,
          "aa_end": null,
          "aa_length": 1369,
          "cds_start": 4000,
          "cds_end": null,
          "cds_length": 4110,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000251636.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_019030.4"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "c.4000G>A",
          "hgvs_p": "p.Val1334Ile",
          "transcript": "ENST00000251636.10",
          "protein_id": "ENSP00000251636.5",
          "transcript_support_level": 1,
          "aa_start": 1334,
          "aa_end": null,
          "aa_length": 1369,
          "cds_start": 4000,
          "cds_end": null,
          "cds_length": 4110,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_019030.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000251636.10"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "n.4208G>A",
          "hgvs_p": null,
          "transcript": "ENST00000504778.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000504778.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "c.4018G>A",
          "hgvs_p": "p.Val1340Ile",
          "transcript": "ENST00000867273.1",
          "protein_id": "ENSP00000537332.1",
          "transcript_support_level": null,
          "aa_start": 1340,
          "aa_end": null,
          "aa_length": 1375,
          "cds_start": 4018,
          "cds_end": null,
          "cds_length": 4128,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867273.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "c.3952G>A",
          "hgvs_p": "p.Val1318Ile",
          "transcript": "ENST00000912931.1",
          "protein_id": "ENSP00000582990.1",
          "transcript_support_level": null,
          "aa_start": 1318,
          "aa_end": null,
          "aa_length": 1353,
          "cds_start": 3952,
          "cds_end": null,
          "cds_length": 4062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912931.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "c.3931G>A",
          "hgvs_p": "p.Val1311Ile",
          "transcript": "ENST00000962095.1",
          "protein_id": "ENSP00000632154.1",
          "transcript_support_level": null,
          "aa_start": 1311,
          "aa_end": null,
          "aa_length": 1346,
          "cds_start": 3931,
          "cds_end": null,
          "cds_length": 4041,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000962095.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "c.3886G>A",
          "hgvs_p": "p.Val1296Ile",
          "transcript": "ENST00000867272.1",
          "protein_id": "ENSP00000537331.1",
          "transcript_support_level": null,
          "aa_start": 1296,
          "aa_end": null,
          "aa_length": 1331,
          "cds_start": 3886,
          "cds_end": null,
          "cds_length": 3996,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867272.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "c.3847G>A",
          "hgvs_p": "p.Val1283Ile",
          "transcript": "NM_001345964.2",
          "protein_id": "NP_001332893.1",
          "transcript_support_level": null,
          "aa_start": 1283,
          "aa_end": null,
          "aa_length": 1318,
          "cds_start": 3847,
          "cds_end": null,
          "cds_length": 3957,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001345964.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "c.3847G>A",
          "hgvs_p": "p.Val1283Ile",
          "transcript": "ENST00000867274.1",
          "protein_id": "ENSP00000537333.1",
          "transcript_support_level": null,
          "aa_start": 1283,
          "aa_end": null,
          "aa_length": 1318,
          "cds_start": 3847,
          "cds_end": null,
          "cds_length": 3957,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867274.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "c.3841G>A",
          "hgvs_p": "p.Val1281Ile",
          "transcript": "ENST00000962096.1",
          "protein_id": "ENSP00000632155.1",
          "transcript_support_level": null,
          "aa_start": 1281,
          "aa_end": null,
          "aa_length": 1316,
          "cds_start": 3841,
          "cds_end": null,
          "cds_length": 3951,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000962096.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "c.3697G>A",
          "hgvs_p": "p.Val1233Ile",
          "transcript": "ENST00000867275.1",
          "protein_id": "ENSP00000537334.1",
          "transcript_support_level": null,
          "aa_start": 1233,
          "aa_end": null,
          "aa_length": 1268,
          "cds_start": 3697,
          "cds_end": null,
          "cds_length": 3807,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867275.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "c.3565G>A",
          "hgvs_p": "p.Val1189Ile",
          "transcript": "ENST00000867276.1",
          "protein_id": "ENSP00000537335.1",
          "transcript_support_level": null,
          "aa_start": 1189,
          "aa_end": null,
          "aa_length": 1224,
          "cds_start": 3565,
          "cds_end": null,
          "cds_length": 3675,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867276.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "c.2092G>A",
          "hgvs_p": "p.Val698Ile",
          "transcript": "NM_001345965.2",
          "protein_id": "NP_001332894.1",
          "transcript_support_level": null,
          "aa_start": 698,
          "aa_end": null,
          "aa_length": 733,
          "cds_start": 2092,
          "cds_end": null,
          "cds_length": 2202,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001345965.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": 23,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "c.3563-3365G>A",
          "hgvs_p": null,
          "transcript": "ENST00000912930.1",
          "protein_id": "ENSP00000582989.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1204,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912930.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "n.4201G>A",
          "hgvs_p": null,
          "transcript": "NR_144323.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_144323.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "n.4170G>A",
          "hgvs_p": null,
          "transcript": "NR_144324.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_144324.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX29",
          "gene_hgnc_id": 15815,
          "hgvs_c": "n.4017G>A",
          "hgvs_p": null,
          "transcript": "NR_144325.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_144325.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CCNO-DT",
          "gene_hgnc_id": 55543,
          "hgvs_c": "n.129-19628C>T",
          "hgvs_p": null,
          "transcript": "ENST00000506435.2",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000506435.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CCNO-DT",
          "gene_hgnc_id": 55543,
          "hgvs_c": "n.148-19628C>T",
          "hgvs_p": null,
          "transcript": "ENST00000749850.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000749850.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CCNO-DT",
          "gene_hgnc_id": 55543,
          "hgvs_c": "n.107-19628C>T",
          "hgvs_p": null,
          "transcript": "ENST00000749851.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000749851.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
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        {
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          "biotype": "pseudogene",
          "feature": "NR_185977.1"
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      ],
      "gene_symbol": "DHX29",
      "gene_hgnc_id": 15815,
      "dbsnp": "rs141019647",
      "frequency_reference_population": 0.000109143766,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 176,
      "gnomad_exomes_af": 0.000101342,
      "gnomad_genomes_af": 0.000184031,
      "gnomad_exomes_ac": 148,
      "gnomad_genomes_ac": 28,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.09097343683242798,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.066,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0673,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.52,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.796,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_019030.4",
          "gene_symbol": "DHX29",
          "hgnc_id": 15815,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.4000G>A",
          "hgvs_p": "p.Val1334Ile"
        },
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000506435.2",
          "gene_symbol": "CCNO-DT",
          "hgnc_id": 55543,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.129-19628C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}