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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-56911296-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=56911296&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 56911296,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000285947.5",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "c.226T>G",
          "hgvs_p": "p.Ser76Ala",
          "transcript": "NM_153706.4",
          "protein_id": "NP_714917.2",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 299,
          "cds_start": 226,
          "cds_end": null,
          "cds_length": 900,
          "cdna_start": 276,
          "cdna_end": null,
          "cdna_length": 1396,
          "mane_select": "ENST00000285947.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "c.226T>G",
          "hgvs_p": "p.Ser76Ala",
          "transcript": "ENST00000285947.5",
          "protein_id": "ENSP00000285947.2",
          "transcript_support_level": 1,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 299,
          "cds_start": 226,
          "cds_end": null,
          "cds_length": 900,
          "cdna_start": 276,
          "cdna_end": null,
          "cdna_length": 1396,
          "mane_select": "NM_153706.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "c.226T>G",
          "hgvs_p": "p.Ser76Ala",
          "transcript": "ENST00000628593.1",
          "protein_id": "ENSP00000486609.1",
          "transcript_support_level": 1,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 271,
          "cds_start": 226,
          "cds_end": null,
          "cds_length": 816,
          "cdna_start": 520,
          "cdna_end": null,
          "cdna_length": 1306,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "c.148T>G",
          "hgvs_p": "p.Ser50Ala",
          "transcript": "NM_001323018.2",
          "protein_id": "NP_001309947.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 148,
          "cds_end": null,
          "cds_length": 822,
          "cdna_start": 466,
          "cdna_end": null,
          "cdna_length": 1586,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "c.226T>G",
          "hgvs_p": "p.Ser76Ala",
          "transcript": "NM_001171990.3",
          "protein_id": "NP_001165461.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 271,
          "cds_start": 226,
          "cds_end": null,
          "cds_length": 816,
          "cdna_start": 276,
          "cdna_end": null,
          "cdna_length": 4430,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "c.226T>G",
          "hgvs_p": "p.Ser76Ala",
          "transcript": "NM_001323019.2",
          "protein_id": "NP_001309948.1",
          "transcript_support_level": null,
          "aa_start": 76,
          "aa_end": null,
          "aa_length": 250,
          "cds_start": 226,
          "cds_end": null,
          "cds_length": 753,
          "cdna_start": 276,
          "cdna_end": null,
          "cdna_length": 1406,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "c.148T>G",
          "hgvs_p": "p.Ser50Ala",
          "transcript": "NM_001323020.2",
          "protein_id": "NP_001309949.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 224,
          "cds_start": 148,
          "cds_end": null,
          "cds_length": 675,
          "cdna_start": 466,
          "cdna_end": null,
          "cdna_length": 1596,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "c.130T>G",
          "hgvs_p": "p.Ser44Ala",
          "transcript": "XM_011543139.3",
          "protein_id": "XP_011541441.1",
          "transcript_support_level": null,
          "aa_start": 44,
          "aa_end": null,
          "aa_length": 267,
          "cds_start": 130,
          "cds_end": null,
          "cds_length": 804,
          "cdna_start": 625,
          "cdna_end": null,
          "cdna_length": 1745,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "n.248T>G",
          "hgvs_p": null,
          "transcript": "ENST00000480414.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 560,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "n.279T>G",
          "hgvs_p": null,
          "transcript": "ENST00000498322.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 571,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "c.-3T>G",
          "hgvs_p": null,
          "transcript": "XM_011543140.4",
          "protein_id": "XP_011541442.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 223,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 672,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1357,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "c.-929T>G",
          "hgvs_p": null,
          "transcript": "XM_005248426.5",
          "protein_id": "XP_005248483.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 155,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 468,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2079,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "c.-218-13T>G",
          "hgvs_p": null,
          "transcript": "NM_001323022.1",
          "protein_id": "NP_001309951.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1299,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "n.98+1553T>G",
          "hgvs_p": null,
          "transcript": "ENST00000418299.5",
          "protein_id": "ENSP00000398449.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1009,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "n.335+902T>G",
          "hgvs_p": null,
          "transcript": "ENST00000463805.5",
          "protein_id": null,
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          "aa_start": null,
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          "cds_start": -4,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "n.378+185T>G",
          "hgvs_p": null,
          "transcript": "ENST00000472636.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 606,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "n.202-13T>G",
          "hgvs_p": null,
          "transcript": "ENST00000475908.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 905,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "n.216+185T>G",
          "hgvs_p": null,
          "transcript": "ENST00000477359.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
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          "cds_start": -4,
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          "cdna_start": null,
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          "cdna_length": 449,
          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "n.148+1553T>G",
          "hgvs_p": null,
          "transcript": "NR_136555.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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          "cds_start": -4,
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          "cdna_start": null,
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          "cdna_length": 4062,
          "mane_select": null,
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        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "n.148+1553T>G",
          "hgvs_p": null,
          "transcript": "NR_136556.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1028,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "n.333+902T>G",
          "hgvs_p": null,
          "transcript": "NR_136557.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1203,
          "mane_select": null,
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        },
        {
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          "protein_coding": false,
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          "exon_count": 4,
          "intron_rank": 1,
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          "hgvs_c": "n.148+1553T>G",
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          "transcript": "NR_136558.2",
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          "aa_length": null,
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        },
        {
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          "protein_coding": true,
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          "consequences": [
            "downstream_gene_variant"
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          "exon_count": 2,
          "intron_rank": null,
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          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "c.*1T>G",
          "hgvs_p": null,
          "transcript": "ENST00000423328.1",
          "protein_id": "ENSP00000406183.1",
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          "aa_start": null,
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          "cds_start": -4,
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          "cds_length": 147,
          "cdna_start": null,
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          "cdna_length": 417,
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        },
        {
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          "protein_coding": false,
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          "consequences": [
            "downstream_gene_variant"
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          "exon_rank": null,
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          "exon_count": 1,
          "intron_rank": null,
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          "gene_symbol": "SETD9",
          "gene_hgnc_id": 28508,
          "hgvs_c": "n.*1T>G",
          "hgvs_p": null,
          "transcript": "ENST00000624733.1",
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          "transcript_support_level": 6,
          "aa_start": null,
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          "aa_length": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 384,
          "mane_select": null,
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      ],
      "gene_symbol": "SETD9",
      "gene_hgnc_id": 28508,
      "dbsnp": "rs2257505",
      "frequency_reference_population": 0.0000027391632,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 4,
      "gnomad_exomes_af": 0.00000273916,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.018361002206802368,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.026,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0585,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.8,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.061,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000285947.5",
          "gene_symbol": "SETD9",
          "hgnc_id": 28508,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.226T>G",
          "hgvs_p": "p.Ser76Ala"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}