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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-68293790-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=68293790&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 68293790,
      "ref": "C",
      "alt": "T",
      "effect": "stop_gained",
      "transcript": "NM_181523.3",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*",
          "transcript": "NM_181523.3",
          "protein_id": "NP_852664.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000521381.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_181523.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*",
          "transcript": "ENST00000521381.6",
          "protein_id": "ENSP00000428056.1",
          "transcript_support_level": 1,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_181523.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000521381.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.571C>T",
          "hgvs_p": "p.Arg191*",
          "transcript": "ENST00000336483.10",
          "protein_id": "ENSP00000338554.5",
          "transcript_support_level": 1,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 454,
          "cds_start": 571,
          "cds_end": null,
          "cds_length": 1365,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000336483.10"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.481C>T",
          "hgvs_p": "p.Arg161*",
          "transcript": "ENST00000320694.13",
          "protein_id": "ENSP00000323512.8",
          "transcript_support_level": 1,
          "aa_start": 161,
          "aa_end": null,
          "aa_length": 424,
          "cds_start": 481,
          "cds_end": null,
          "cds_length": 1275,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000320694.13"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*",
          "transcript": "ENST00000697461.1",
          "protein_id": "ENSP00000513319.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 732,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 2199,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000697461.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*",
          "transcript": "ENST00000870050.1",
          "protein_id": "ENSP00000540109.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 732,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 2199,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000870050.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*",
          "transcript": "ENST00000517643.2",
          "protein_id": "ENSP00000513333.1",
          "transcript_support_level": 3,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000517643.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*",
          "transcript": "ENST00000521657.6",
          "protein_id": "ENSP00000429277.1",
          "transcript_support_level": 5,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000521657.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*",
          "transcript": "ENST00000697458.1",
          "protein_id": "ENSP00000513316.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000697458.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*",
          "transcript": "ENST00000870051.1",
          "protein_id": "ENSP00000540110.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000870051.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*",
          "transcript": "ENST00000870052.1",
          "protein_id": "ENSP00000540111.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000870052.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*",
          "transcript": "ENST00000870053.1",
          "protein_id": "ENSP00000540112.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000870053.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*",
          "transcript": "ENST00000961623.1",
          "protein_id": "ENSP00000631682.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961623.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*",
          "transcript": "ENST00000870049.1",
          "protein_id": "ENSP00000540108.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 705,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 2118,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
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          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1306C>T",
          "hgvs_p": "p.Arg436*",
          "transcript": "ENST00000697457.1",
          "protein_id": "ENSP00000513315.1",
          "transcript_support_level": null,
          "aa_start": 436,
          "aa_end": null,
          "aa_length": 699,
          "cds_start": 1306,
          "cds_end": null,
          "cds_length": 2100,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000697457.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*",
          "transcript": "ENST00000961622.1",
          "protein_id": "ENSP00000631681.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 667,
          "cds_start": 1381,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000961622.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.856C>T",
          "hgvs_p": "p.Arg286*",
          "transcript": "ENST00000697460.1",
          "protein_id": "ENSP00000513318.1",
          "transcript_support_level": null,
          "aa_start": 286,
          "aa_end": null,
          "aa_length": 549,
          "cds_start": 856,
          "cds_end": null,
          "cds_length": 1650,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.571C>T",
          "hgvs_p": "p.Arg191*",
          "transcript": "ENST00000697462.1",
          "protein_id": "ENSP00000513320.1",
          "transcript_support_level": null,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 462,
          "cds_start": 571,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000697462.1"
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.571C>T",
          "hgvs_p": "p.Arg191*",
          "transcript": "NM_181504.4",
          "protein_id": "NP_852556.2",
          "transcript_support_level": null,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 454,
          "cds_start": 571,
          "cds_end": null,
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          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_181504.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.571C>T",
          "hgvs_p": "p.Arg191*",
          "transcript": "ENST00000522084.6",
          "protein_id": "ENSP00000429766.2",
          "transcript_support_level": 3,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 454,
          "cds_start": 571,
          "cds_end": null,
          "cds_length": 1365,
          "cdna_start": null,
          "cdna_end": null,
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        {
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          "exon_count": 9,
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          "transcript": "ENST00000517698.6",
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        },
        {
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          "protein_coding": false,
          "strand": true,
          "consequences": [
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          "exon_rank": 5,
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          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "PIK3R1",
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          "hgvs_c": "n.*347C>T",
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          "transcript": "ENST00000697464.1",
          "protein_id": "ENSP00000513322.1",
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          "biotype": "nonsense_mediated_decay",
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        },
        {
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          "protein_coding": true,
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          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
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          "exon_count": 5,
          "intron_rank": null,
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          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.-24C>T",
          "hgvs_p": null,
          "transcript": "ENST00000697470.1",
          "protein_id": "ENSP00000513328.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 235,
          "cds_start": null,
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          "cds_length": 708,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000697470.1"
        },
        {
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          "protein_coding": true,
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          "consequences": [
            "downstream_gene_variant"
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          "exon_count": 9,
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          "gene_symbol": "PIK3R1",
          "gene_hgnc_id": 8979,
          "hgvs_c": "c.*68C>T",
          "hgvs_p": null,
          "transcript": "ENST00000697556.1",
          "protein_id": "ENSP00000513334.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 405,
          "cds_start": null,
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          "cds_length": 1220,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000697556.1"
        }
      ],
      "gene_symbol": "PIK3R1",
      "gene_hgnc_id": 8979,
      "dbsnp": "rs1057519838",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": 0,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 0,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.3700000047683716,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.37,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 0.562,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 18,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 18,
          "benign_score": 0,
          "pathogenic_score": 18,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "NM_181523.3",
          "gene_symbol": "PIK3R1",
          "hgnc_id": 8979,
          "effects": [
            "stop_gained"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461*"
        }
      ],
      "clinvar_disease": " autosomal recessive,Agammaglobulinemia 7,Immunodeficiency 36 with lymphoproliferation,SHORT syndrome",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:1 LP:1",
      "phenotype_combined": "SHORT syndrome|Agammaglobulinemia 7, autosomal recessive;SHORT syndrome;Immunodeficiency 36 with lymphoproliferation",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}