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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-72226172-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=72226172&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 72226172,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001286748.2",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.722G>A",
          "hgvs_p": "p.Arg241Gln",
          "transcript": "NM_015084.3",
          "protein_id": "NP_055899.2",
          "transcript_support_level": null,
          "aa_start": 241,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 722,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000261413.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_015084.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.722G>A",
          "hgvs_p": "p.Arg241Gln",
          "transcript": "ENST00000261413.10",
          "protein_id": "ENSP00000261413.5",
          "transcript_support_level": 1,
          "aa_start": 241,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 722,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_015084.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000261413.10"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "n.*150G>A",
          "hgvs_p": null,
          "transcript": "ENST00000522562.5",
          "protein_id": "ENSP00000511937.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000522562.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "n.*150G>A",
          "hgvs_p": null,
          "transcript": "ENST00000522562.5",
          "protein_id": "ENSP00000511937.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000522562.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.722G>A",
          "hgvs_p": "p.Arg241Gln",
          "transcript": "ENST00000695404.1",
          "protein_id": "ENSP00000511886.1",
          "transcript_support_level": null,
          "aa_start": 241,
          "aa_end": null,
          "aa_length": 460,
          "cds_start": 722,
          "cds_end": null,
          "cds_length": 1383,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000695404.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.722G>A",
          "hgvs_p": "p.Arg241Gln",
          "transcript": "ENST00000694998.2",
          "protein_id": "ENSP00000511636.2",
          "transcript_support_level": null,
          "aa_start": 241,
          "aa_end": null,
          "aa_length": 436,
          "cds_start": 722,
          "cds_end": null,
          "cds_length": 1311,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000694998.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.767G>A",
          "hgvs_p": "p.Arg256Gln",
          "transcript": "ENST00000926538.1",
          "protein_id": "ENSP00000596597.1",
          "transcript_support_level": null,
          "aa_start": 256,
          "aa_end": null,
          "aa_length": 429,
          "cds_start": 767,
          "cds_end": null,
          "cds_length": 1290,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000926538.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.764G>A",
          "hgvs_p": "p.Arg255Gln",
          "transcript": "NM_001286748.2",
          "protein_id": "NP_001273677.1",
          "transcript_support_level": null,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 428,
          "cds_start": 764,
          "cds_end": null,
          "cds_length": 1287,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001286748.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.764G>A",
          "hgvs_p": "p.Arg255Gln",
          "transcript": "ENST00000513900.6",
          "protein_id": "ENSP00000426941.1",
          "transcript_support_level": 2,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 428,
          "cds_start": 764,
          "cds_end": null,
          "cds_length": 1287,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000513900.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.737G>A",
          "hgvs_p": "p.Arg246Gln",
          "transcript": "ENST00000953797.1",
          "protein_id": "ENSP00000623856.1",
          "transcript_support_level": null,
          "aa_start": 246,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 737,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000953797.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.722G>A",
          "hgvs_p": "p.Arg241Gln",
          "transcript": "ENST00000694989.1",
          "protein_id": "ENSP00000511631.1",
          "transcript_support_level": null,
          "aa_start": 241,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 722,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000694989.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.722G>A",
          "hgvs_p": "p.Arg241Gln",
          "transcript": "ENST00000694990.1",
          "protein_id": "ENSP00000511632.1",
          "transcript_support_level": null,
          "aa_start": 241,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 722,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000694990.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.683G>A",
          "hgvs_p": "p.Arg228Gln",
          "transcript": "ENST00000695403.1",
          "protein_id": "ENSP00000511885.1",
          "transcript_support_level": null,
          "aa_start": 228,
          "aa_end": null,
          "aa_length": 401,
          "cds_start": 683,
          "cds_end": null,
          "cds_length": 1206,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000695403.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.599G>A",
          "hgvs_p": "p.Arg200Gln",
          "transcript": "ENST00000694994.1",
          "protein_id": "ENSP00000511933.1",
          "transcript_support_level": null,
          "aa_start": 200,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": 599,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000694994.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.644G>A",
          "hgvs_p": "p.Arg215Gln",
          "transcript": "ENST00000695298.1",
          "protein_id": "ENSP00000511790.1",
          "transcript_support_level": null,
          "aa_start": 215,
          "aa_end": null,
          "aa_length": 388,
          "cds_start": 644,
          "cds_end": null,
          "cds_length": 1167,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000695298.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.599G>A",
          "hgvs_p": "p.Arg200Gln",
          "transcript": "ENST00000694995.1",
          "protein_id": "ENSP00000511935.1",
          "transcript_support_level": null,
          "aa_start": 200,
          "aa_end": null,
          "aa_length": 373,
          "cds_start": 599,
          "cds_end": null,
          "cds_length": 1122,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000694995.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.554G>A",
          "hgvs_p": "p.Arg185Gln",
          "transcript": "NM_001286751.2",
          "protein_id": "NP_001273680.1",
          "transcript_support_level": null,
          "aa_start": 185,
          "aa_end": null,
          "aa_length": 358,
          "cds_start": 554,
          "cds_end": null,
          "cds_length": 1077,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001286751.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.554G>A",
          "hgvs_p": "p.Arg185Gln",
          "transcript": "ENST00000457646.9",
          "protein_id": "ENSP00000428120.2",
          "transcript_support_level": 2,
          "aa_start": 185,
          "aa_end": null,
          "aa_length": 358,
          "cds_start": 554,
          "cds_end": null,
          "cds_length": 1077,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000457646.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.554G>A",
          "hgvs_p": "p.Arg185Gln",
          "transcript": "ENST00000519143.6",
          "protein_id": "ENSP00000511934.1",
          "transcript_support_level": 5,
          "aa_start": 185,
          "aa_end": null,
          "aa_length": 358,
          "cds_start": 554,
          "cds_end": null,
          "cds_length": 1077,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000519143.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MRPS27",
          "gene_hgnc_id": 14512,
          "hgvs_c": "c.554G>A",
          "hgvs_p": "p.Arg185Gln",
          "transcript": "ENST00000694996.2",
          "protein_id": "ENSP00000511936.1",
          "transcript_support_level": null,
          "aa_start": 185,
          "aa_end": null,
          "aa_length": 358,
          "cds_start": 554,
          "cds_end": null,
          "cds_length": 1077,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000694996.2"
        },
        {
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        {
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      ],
      "gene_symbol": "MRPS27",
      "gene_hgnc_id": 14512,
      "dbsnp": null,
      "frequency_reference_population": 0.0000027368656,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 4,
      "gnomad_exomes_af": 0.00000273687,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.4081862270832062,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.221,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1505,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.32,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.367,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001286748.2",
          "gene_symbol": "MRPS27",
          "hgnc_id": 14512,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.764G>A",
          "hgvs_p": "p.Arg255Gln"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}