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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-73901264-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=73901264&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 73901264,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001080479.3",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.4054G>A",
          "hgvs_p": "p.Val1352Ile",
          "transcript": "NM_001177693.2",
          "protein_id": "NP_001171164.1",
          "transcript_support_level": null,
          "aa_start": 1352,
          "aa_end": null,
          "aa_length": 1705,
          "cds_start": 4054,
          "cds_end": null,
          "cds_length": 5118,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000513042.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001177693.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.4054G>A",
          "hgvs_p": "p.Val1352Ile",
          "transcript": "ENST00000513042.7",
          "protein_id": "ENSP00000441436.1",
          "transcript_support_level": 5,
          "aa_start": 1352,
          "aa_end": null,
          "aa_length": 1705,
          "cds_start": 4054,
          "cds_end": null,
          "cds_length": 5118,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001177693.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000513042.7"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.4054G>A",
          "hgvs_p": "p.Val1352Ile",
          "transcript": "ENST00000437974.5",
          "protein_id": "ENSP00000411459.1",
          "transcript_support_level": 1,
          "aa_start": 1352,
          "aa_end": null,
          "aa_length": 1731,
          "cds_start": 4054,
          "cds_end": null,
          "cds_length": 5196,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000437974.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.4054G>A",
          "hgvs_p": "p.Val1352Ile",
          "transcript": "ENST00000426542.6",
          "protein_id": "ENSP00000412175.2",
          "transcript_support_level": 1,
          "aa_start": 1352,
          "aa_end": null,
          "aa_length": 1705,
          "cds_start": 4054,
          "cds_end": null,
          "cds_length": 5118,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000426542.6"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 37,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.4054G>A",
          "hgvs_p": "p.Val1352Ile",
          "transcript": "NM_001080479.3",
          "protein_id": "NP_001073948.2",
          "transcript_support_level": null,
          "aa_start": 1352,
          "aa_end": null,
          "aa_length": 1731,
          "cds_start": 4054,
          "cds_end": null,
          "cds_length": 5196,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001080479.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 37,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.4054G>A",
          "hgvs_p": "p.Val1352Ile",
          "transcript": "ENST00000545377.5",
          "protein_id": "ENSP00000441913.1",
          "transcript_support_level": 5,
          "aa_start": 1352,
          "aa_end": null,
          "aa_length": 1731,
          "cds_start": 4054,
          "cds_end": null,
          "cds_length": 5196,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000545377.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 37,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.4054G>A",
          "hgvs_p": "p.Val1352Ile",
          "transcript": "ENST00000948319.1",
          "protein_id": "ENSP00000618378.1",
          "transcript_support_level": null,
          "aa_start": 1352,
          "aa_end": null,
          "aa_length": 1731,
          "cds_start": 4054,
          "cds_end": null,
          "cds_length": 5196,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000948319.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.4054G>A",
          "hgvs_p": "p.Val1352Ile",
          "transcript": "ENST00000872894.1",
          "protein_id": "ENSP00000542953.1",
          "transcript_support_level": null,
          "aa_start": 1352,
          "aa_end": null,
          "aa_length": 1705,
          "cds_start": 4054,
          "cds_end": null,
          "cds_length": 5118,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872894.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.3949G>A",
          "hgvs_p": "p.Val1317Ile",
          "transcript": "ENST00000872896.1",
          "protein_id": "ENSP00000542955.1",
          "transcript_support_level": null,
          "aa_start": 1317,
          "aa_end": null,
          "aa_length": 1696,
          "cds_start": 3949,
          "cds_end": null,
          "cds_length": 5091,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872896.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.3940G>A",
          "hgvs_p": "p.Val1314Ile",
          "transcript": "ENST00000872895.1",
          "protein_id": "ENSP00000542954.1",
          "transcript_support_level": null,
          "aa_start": 1314,
          "aa_end": null,
          "aa_length": 1667,
          "cds_start": 3940,
          "cds_end": null,
          "cds_length": 5004,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872895.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.4054G>A",
          "hgvs_p": "p.Val1352Ile",
          "transcript": "NM_001388078.1",
          "protein_id": "NP_001375007.1",
          "transcript_support_level": null,
          "aa_start": 1352,
          "aa_end": null,
          "aa_length": 1651,
          "cds_start": 4054,
          "cds_end": null,
          "cds_length": 4956,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001388078.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.4054G>A",
          "hgvs_p": "p.Val1352Ile",
          "transcript": "ENST00000296794.10",
          "protein_id": "ENSP00000296794.6",
          "transcript_support_level": 5,
          "aa_start": 1352,
          "aa_end": null,
          "aa_length": 1651,
          "cds_start": 4054,
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          "cds_length": 4956,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000296794.10"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.3760G>A",
          "hgvs_p": "p.Val1254Ile",
          "transcript": "NM_001388076.1",
          "protein_id": "NP_001375005.1",
          "transcript_support_level": null,
          "aa_start": 1254,
          "aa_end": null,
          "aa_length": 1607,
          "cds_start": 3760,
          "cds_end": null,
          "cds_length": 4824,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001388076.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.3760G>A",
          "hgvs_p": "p.Val1254Ile",
          "transcript": "ENST00000872897.1",
          "protein_id": "ENSP00000542956.1",
          "transcript_support_level": null,
          "aa_start": 1254,
          "aa_end": null,
          "aa_length": 1607,
          "cds_start": 3760,
          "cds_end": null,
          "cds_length": 4824,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872897.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.3115G>A",
          "hgvs_p": "p.Val1039Ile",
          "transcript": "NM_001244364.2",
          "protein_id": "NP_001231293.1",
          "transcript_support_level": null,
          "aa_start": 1039,
          "aa_end": null,
          "aa_length": 1392,
          "cds_start": 3115,
          "cds_end": null,
          "cds_length": 4179,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001244364.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.3115G>A",
          "hgvs_p": "p.Val1039Ile",
          "transcript": "ENST00000296799.8",
          "protein_id": "ENSP00000296799.4",
          "transcript_support_level": 2,
          "aa_start": 1039,
          "aa_end": null,
          "aa_length": 1392,
          "cds_start": 3115,
          "cds_end": null,
          "cds_length": 4179,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000296799.8"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "c.814G>A",
          "hgvs_p": "p.Val272Ile",
          "transcript": "ENST00000512883.1",
          "protein_id": "ENSP00000421081.1",
          "transcript_support_level": 2,
          "aa_start": 272,
          "aa_end": null,
          "aa_length": 651,
          "cds_start": 814,
          "cds_end": null,
          "cds_length": 1956,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000512883.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ARHGEF28",
          "gene_hgnc_id": 30322,
          "hgvs_c": "n.4323G>A",
          "hgvs_p": null,
          "transcript": "ENST00000510312.6",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000510312.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000304540",
          "gene_hgnc_id": 59068,
          "hgvs_c": "n.79-4100C>T",
          "hgvs_p": null,
          "transcript": "ENST00000804413.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000804413.1"
        }
      ],
      "gene_symbol": "ARHGEF28",
      "gene_hgnc_id": 30322,
      "dbsnp": "rs188040167",
      "frequency_reference_population": 0.0039219675,
      "hom_count_reference_population": 13,
      "allele_count_reference_population": 6326,
      "gnomad_exomes_af": 0.00400335,
      "gnomad_genomes_af": 0.00314085,
      "gnomad_exomes_ac": 5848,
      "gnomad_genomes_ac": 478,
      "gnomad_exomes_homalt": 12,
      "gnomad_genomes_homalt": 1,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.004440754652023315,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.07,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0886,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.64,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.26,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -16,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BS2",
      "acmg_by_gene": [
        {
          "score": -16,
          "benign_score": 16,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_001080479.3",
          "gene_symbol": "ARHGEF28",
          "hgnc_id": 30322,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR,SD",
          "hgvs_c": "c.4054G>A",
          "hgvs_p": "p.Val1352Ile"
        },
        {
          "score": -16,
          "benign_score": 16,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000804413.1",
          "gene_symbol": "ENSG00000304540",
          "hgnc_id": 59068,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.79-4100C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not provided,not specified",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:3",
      "phenotype_combined": "not specified|not provided",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}
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