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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-75590351-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=75590351&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 75590351,
      "ref": "A",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000241436.9",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.1267A>C",
          "hgvs_p": "p.Ser423Arg",
          "transcript": "NM_016218.6",
          "protein_id": "NP_057302.1",
          "transcript_support_level": null,
          "aa_start": 423,
          "aa_end": null,
          "aa_length": 870,
          "cds_start": 1267,
          "cds_end": null,
          "cds_length": 2613,
          "cdna_start": 1304,
          "cdna_end": null,
          "cdna_length": 14623,
          "mane_select": "ENST00000241436.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.1267A>C",
          "hgvs_p": "p.Ser423Arg",
          "transcript": "ENST00000241436.9",
          "protein_id": "ENSP00000241436.4",
          "transcript_support_level": 1,
          "aa_start": 423,
          "aa_end": null,
          "aa_length": 870,
          "cds_start": 1267,
          "cds_end": null,
          "cds_length": 2613,
          "cdna_start": 1304,
          "cdna_end": null,
          "cdna_length": 14623,
          "mane_select": "NM_016218.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.1267A>C",
          "hgvs_p": "p.Ser423Arg",
          "transcript": "ENST00000515295.5",
          "protein_id": "ENSP00000424174.1",
          "transcript_support_level": 1,
          "aa_start": 423,
          "aa_end": null,
          "aa_length": 472,
          "cds_start": 1267,
          "cds_end": null,
          "cds_length": 1419,
          "cdna_start": 1280,
          "cdna_end": null,
          "cdna_length": 1438,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.1267A>C",
          "hgvs_p": "p.Ser423Arg",
          "transcript": "ENST00000504026.5",
          "protein_id": "ENSP00000425075.1",
          "transcript_support_level": 1,
          "aa_start": 423,
          "aa_end": null,
          "aa_length": 461,
          "cds_start": 1267,
          "cds_end": null,
          "cds_length": 1386,
          "cdna_start": 1267,
          "cdna_end": null,
          "cdna_length": 1484,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.935-5871A>C",
          "hgvs_p": null,
          "transcript": "ENST00000508526.5",
          "protein_id": "ENSP00000426853.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 672,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2019,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2019,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "n.1259+3293A>C",
          "hgvs_p": null,
          "transcript": "ENST00000514141.5",
          "protein_id": "ENSP00000423526.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4345,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.1309A>C",
          "hgvs_p": "p.Ser437Arg",
          "transcript": "NM_001387111.3",
          "protein_id": "NP_001374040.1",
          "transcript_support_level": null,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 884,
          "cds_start": 1309,
          "cds_end": null,
          "cds_length": 2655,
          "cdna_start": 1346,
          "cdna_end": null,
          "cdna_length": 14665,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.1309A>C",
          "hgvs_p": "p.Ser437Arg",
          "transcript": "NM_001395894.1",
          "protein_id": "NP_001382823.1",
          "transcript_support_level": null,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 884,
          "cds_start": 1309,
          "cds_end": null,
          "cds_length": 2655,
          "cdna_start": 1483,
          "cdna_end": null,
          "cdna_length": 14802,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.1306A>C",
          "hgvs_p": "p.Ser436Arg",
          "transcript": "NM_001395897.1",
          "protein_id": "NP_001382826.1",
          "transcript_support_level": null,
          "aa_start": 436,
          "aa_end": null,
          "aa_length": 883,
          "cds_start": 1306,
          "cds_end": null,
          "cds_length": 2652,
          "cdna_start": 1480,
          "cdna_end": null,
          "cdna_length": 14799,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.1267A>C",
          "hgvs_p": "p.Ser423Arg",
          "transcript": "NM_001387113.3",
          "protein_id": "NP_001374042.1",
          "transcript_support_level": null,
          "aa_start": 423,
          "aa_end": null,
          "aa_length": 870,
          "cds_start": 1267,
          "cds_end": null,
          "cds_length": 2613,
          "cdna_start": 1441,
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          "cdna_length": 14760,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
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          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.1114A>C",
          "hgvs_p": "p.Ser372Arg",
          "transcript": "NM_001395899.1",
          "protein_id": "NP_001382828.1",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 819,
          "cds_start": 1114,
          "cds_end": null,
          "cds_length": 2460,
          "cdna_start": 1151,
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          "cdna_length": 14470,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
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          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.1069A>C",
          "hgvs_p": "p.Ser357Arg",
          "transcript": "NM_001345921.3",
          "protein_id": "NP_001332850.1",
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        {
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          "consequences": [
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          ],
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          "exon_count": 15,
          "intron_rank": null,
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          "gene_symbol": "POLK",
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          "hgvs_c": "c.1069A>C",
          "hgvs_p": "p.Ser357Arg",
          "transcript": "NM_001395900.1",
          "protein_id": "NP_001382829.1",
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        {
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          "strand": true,
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          ],
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          "exon_count": 14,
          "intron_rank": null,
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          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.1027A>C",
          "hgvs_p": "p.Ser343Arg",
          "transcript": "NM_001395901.1",
          "protein_id": "NP_001382830.1",
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          "cds_start": 1027,
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          "cdna_start": 1064,
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        },
        {
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          ],
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        {
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          "exon_count": 17,
          "intron_rank": null,
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          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.997A>C",
          "hgvs_p": "p.Ser333Arg",
          "transcript": "NM_001395893.1",
          "protein_id": "NP_001382822.1",
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        {
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          ],
          "exon_rank": 11,
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          "intron_rank": null,
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          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.997A>C",
          "hgvs_p": "p.Ser333Arg",
          "transcript": "NM_001395902.1",
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        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "POLK",
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          "hgvs_c": "c.1309A>C",
          "hgvs_p": "p.Ser437Arg",
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        {
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          "gene_symbol": "POLK",
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        {
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          ],
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          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.1309A>C",
          "hgvs_p": "p.Ser437Arg",
          "transcript": "XM_054328412.1",
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          "biotype": null,
          "feature": null
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
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          "gene_symbol": "POLK",
          "gene_hgnc_id": 9183,
          "hgvs_c": "c.1309A>C",
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      "allele_count_reference_population": 1369,
      "gnomad_exomes_af": 0.000465137,
      "gnomad_genomes_af": 0.00464823,
      "gnomad_exomes_ac": 661,
      "gnomad_genomes_ac": 708,
      "gnomad_exomes_homalt": 9,
      "gnomad_genomes_homalt": 5,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.009204655885696411,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.058,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2174,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.34,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.731,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -9,
      "acmg_classification": "Benign",
      "acmg_criteria": "PM1,PP2,BP4_Strong,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -9,
          "benign_score": 12,
          "pathogenic_score": 3,
          "criteria": [
            "PM1",
            "PP2",
            "BP4_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000241436.9",
          "gene_symbol": "POLK",
          "hgnc_id": 9183,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1267A>C",
          "hgvs_p": "p.Ser423Arg"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}