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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-96750630-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=96750630&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 96750630,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001750.7",
      "consequences": [
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": 1515,
          "hgvs_c": "c.1472G>C",
          "hgvs_p": "p.Cys491Ser",
          "transcript": "NM_001750.7",
          "protein_id": "NP_001741.4",
          "transcript_support_level": null,
          "aa_start": 491,
          "aa_end": null,
          "aa_length": 791,
          "cds_start": 1472,
          "cds_end": null,
          "cds_length": 2376,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000675179.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001750.7"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": 1515,
          "hgvs_c": "c.1472G>C",
          "hgvs_p": "p.Cys491Ser",
          "transcript": "ENST00000675179.1",
          "protein_id": "ENSP00000501872.1",
          "transcript_support_level": null,
          "aa_start": 491,
          "aa_end": null,
          "aa_length": 791,
          "cds_start": 1472,
          "cds_end": null,
          "cds_length": 2376,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001750.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000675179.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1223G>C",
          "hgvs_p": "p.Cys408Ser",
          "transcript": "ENST00000341926.7",
          "protein_id": "ENSP00000339914.3",
          "transcript_support_level": 1,
          "aa_start": 408,
          "aa_end": null,
          "aa_length": 708,
          "cds_start": 1223,
          "cds_end": null,
          "cds_length": 2127,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000341926.7"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1184G>C",
          "hgvs_p": "p.Cys395Ser",
          "transcript": "ENST00000338252.7",
          "protein_id": "ENSP00000343421.3",
          "transcript_support_level": 1,
          "aa_start": 395,
          "aa_end": null,
          "aa_length": 695,
          "cds_start": 1184,
          "cds_end": null,
          "cds_length": 2088,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000338252.7"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1157G>C",
          "hgvs_p": "p.Cys386Ser",
          "transcript": "ENST00000309190.9",
          "protein_id": "ENSP00000312523.5",
          "transcript_support_level": 1,
          "aa_start": 386,
          "aa_end": null,
          "aa_length": 686,
          "cds_start": 1157,
          "cds_end": null,
          "cds_length": 2061,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000309190.9"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1118G>C",
          "hgvs_p": "p.Cys373Ser",
          "transcript": "ENST00000509903.5",
          "protein_id": "ENSP00000426946.1",
          "transcript_support_level": 1,
          "aa_start": 373,
          "aa_end": null,
          "aa_length": 673,
          "cds_start": 1118,
          "cds_end": null,
          "cds_length": 2022,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000509903.5"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.494G>C",
          "hgvs_p": "p.Cys165Ser",
          "transcript": "ENST00000510500.5",
          "protein_id": "ENSP00000424160.1",
          "transcript_support_level": 1,
          "aa_start": 165,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 494,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000510500.5"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.476G>C",
          "hgvs_p": "p.Cys159Ser",
          "transcript": "ENST00000437034.6",
          "protein_id": "ENSP00000412374.2",
          "transcript_support_level": 1,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 435,
          "cds_start": 476,
          "cds_end": null,
          "cds_length": 1308,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000437034.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "n.1165G>C",
          "hgvs_p": null,
          "transcript": "ENST00000348386.7",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000348386.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "n.392G>C",
          "hgvs_p": null,
          "transcript": "ENST00000484552.6",
          "protein_id": "ENSP00000432878.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000484552.6"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1472G>C",
          "hgvs_p": "p.Cys491Ser",
          "transcript": "ENST00000508830.5",
          "protein_id": "ENSP00000425721.1",
          "transcript_support_level": 5,
          "aa_start": 491,
          "aa_end": null,
          "aa_length": 791,
          "cds_start": 1472,
          "cds_end": null,
          "cds_length": 2376,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000508830.5"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1472G>C",
          "hgvs_p": "p.Cys491Ser",
          "transcript": "ENST00000852912.1",
          "protein_id": "ENSP00000522971.1",
          "transcript_support_level": null,
          "aa_start": 491,
          "aa_end": null,
          "aa_length": 791,
          "cds_start": 1472,
          "cds_end": null,
          "cds_length": 2376,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852912.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1472G>C",
          "hgvs_p": "p.Cys491Ser",
          "transcript": "ENST00000852916.1",
          "protein_id": "ENSP00000522975.1",
          "transcript_support_level": null,
          "aa_start": 491,
          "aa_end": null,
          "aa_length": 791,
          "cds_start": 1472,
          "cds_end": null,
          "cds_length": 2376,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852916.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": 1515,
          "hgvs_c": "c.1415G>C",
          "hgvs_p": "p.Cys472Ser",
          "transcript": "NM_001042441.3",
          "protein_id": "NP_001035906.1",
          "transcript_support_level": null,
          "aa_start": 472,
          "aa_end": null,
          "aa_length": 772,
          "cds_start": 1415,
          "cds_end": null,
          "cds_length": 2319,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001042441.3"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": 1515,
          "hgvs_c": "c.1406G>C",
          "hgvs_p": "p.Cys469Ser",
          "transcript": "NM_001042442.3",
          "protein_id": "NP_001035907.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 1406,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001042442.3"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1406G>C",
          "hgvs_p": "p.Cys469Ser",
          "transcript": "ENST00000674984.1",
          "protein_id": "ENSP00000501713.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 1406,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000674984.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1406G>C",
          "hgvs_p": "p.Cys469Ser",
          "transcript": "ENST00000852909.1",
          "protein_id": "ENSP00000522968.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 1406,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852909.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1406G>C",
          "hgvs_p": "p.Cys469Ser",
          "transcript": "ENST00000852911.1",
          "protein_id": "ENSP00000522970.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 1406,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852911.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1406G>C",
          "hgvs_p": "p.Cys469Ser",
          "transcript": "ENST00000852908.1",
          "protein_id": "ENSP00000522967.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 768,
          "cds_start": 1406,
          "cds_end": null,
          "cds_length": 2307,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852908.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CAST",
          "gene_hgnc_id": null,
          "hgvs_c": "c.1406G>C",
          "hgvs_p": "p.Cys469Ser",
          "transcript": "ENST00000852910.1",
          "protein_id": "ENSP00000522969.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 768,
          "cds_start": 1406,
          "cds_end": null,
          "cds_length": 2307,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852910.1"
        },
        {
          "aa_ref": "C",
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      "gene_symbol": "CAST",
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      "dbsnp": "rs754615",
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      "hom_count_reference_population": 115058,
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      "gnomad_exomes_af": 0.374774,
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      "gnomad_genomes_ac": 52825,
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      "gnomad_genomes_homalt": 9438,
      "gnomad_mito_homoplasmic": null,
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      "computational_score_selected": 0.002227187156677246,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.106,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.052,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.68,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.055,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
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      "mitotip_score": null,
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      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BA1",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
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            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "NM_001750.7",
          "gene_symbol": "CAST",
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          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
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          "hgvs_p": "p.Cys491Ser"
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      ],
      "clinvar_disease": "Peeling skin-leukonuchia-acral punctate keratoses-cheilitis-knuckle pads syndrome,not provided",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:3",
      "phenotype_combined": "Peeling skin-leukonuchia-acral punctate keratoses-cheilitis-knuckle pads syndrome|not provided",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}