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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-107876571-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=107876571&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 107876571,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_007214.5",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.2027C>T",
          "hgvs_p": "p.Thr676Ile",
          "transcript": "NM_007214.5",
          "protein_id": "NP_009145.1",
          "transcript_support_level": null,
          "aa_start": 676,
          "aa_end": null,
          "aa_length": 760,
          "cds_start": 2027,
          "cds_end": null,
          "cds_length": 2283,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000369002.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_007214.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.2027C>T",
          "hgvs_p": "p.Thr676Ile",
          "transcript": "ENST00000369002.9",
          "protein_id": "ENSP00000357998.4",
          "transcript_support_level": 1,
          "aa_start": 676,
          "aa_end": null,
          "aa_length": 760,
          "cds_start": 2027,
          "cds_end": null,
          "cds_length": 2283,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_007214.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000369002.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "n.830C>T",
          "hgvs_p": null,
          "transcript": "ENST00000459782.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000459782.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.2114C>T",
          "hgvs_p": "p.Thr705Ile",
          "transcript": "ENST00000884697.1",
          "protein_id": "ENSP00000554756.1",
          "transcript_support_level": null,
          "aa_start": 705,
          "aa_end": null,
          "aa_length": 789,
          "cds_start": 2114,
          "cds_end": null,
          "cds_length": 2370,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884697.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.2108C>T",
          "hgvs_p": "p.Thr703Ile",
          "transcript": "ENST00000884696.1",
          "protein_id": "ENSP00000554755.1",
          "transcript_support_level": null,
          "aa_start": 703,
          "aa_end": null,
          "aa_length": 787,
          "cds_start": 2108,
          "cds_end": null,
          "cds_length": 2364,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884696.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.2105C>T",
          "hgvs_p": "p.Thr702Ile",
          "transcript": "ENST00000955590.1",
          "protein_id": "ENSP00000625649.1",
          "transcript_support_level": null,
          "aa_start": 702,
          "aa_end": null,
          "aa_length": 786,
          "cds_start": 2105,
          "cds_end": null,
          "cds_length": 2361,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955590.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.2024C>T",
          "hgvs_p": "p.Thr675Ile",
          "transcript": "ENST00000884693.1",
          "protein_id": "ENSP00000554752.1",
          "transcript_support_level": null,
          "aa_start": 675,
          "aa_end": null,
          "aa_length": 759,
          "cds_start": 2024,
          "cds_end": null,
          "cds_length": 2280,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884693.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.1976C>T",
          "hgvs_p": "p.Thr659Ile",
          "transcript": "ENST00000938726.1",
          "protein_id": "ENSP00000608785.1",
          "transcript_support_level": null,
          "aa_start": 659,
          "aa_end": null,
          "aa_length": 743,
          "cds_start": 1976,
          "cds_end": null,
          "cds_length": 2232,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000938726.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.1853C>T",
          "hgvs_p": "p.Thr618Ile",
          "transcript": "ENST00000938728.1",
          "protein_id": "ENSP00000608787.1",
          "transcript_support_level": null,
          "aa_start": 618,
          "aa_end": null,
          "aa_length": 702,
          "cds_start": 1853,
          "cds_end": null,
          "cds_length": 2109,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000938728.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.1799C>T",
          "hgvs_p": "p.Thr600Ile",
          "transcript": "ENST00000955591.1",
          "protein_id": "ENSP00000625650.1",
          "transcript_support_level": null,
          "aa_start": 600,
          "aa_end": null,
          "aa_length": 684,
          "cds_start": 1799,
          "cds_end": null,
          "cds_length": 2055,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955591.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.1766C>T",
          "hgvs_p": "p.Thr589Ile",
          "transcript": "ENST00000884695.1",
          "protein_id": "ENSP00000554754.1",
          "transcript_support_level": null,
          "aa_start": 589,
          "aa_end": null,
          "aa_length": 673,
          "cds_start": 1766,
          "cds_end": null,
          "cds_length": 2022,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884695.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.977C>T",
          "hgvs_p": "p.Thr326Ile",
          "transcript": "ENST00000938727.1",
          "protein_id": "ENSP00000608786.1",
          "transcript_support_level": null,
          "aa_start": 326,
          "aa_end": null,
          "aa_length": 410,
          "cds_start": 977,
          "cds_end": null,
          "cds_length": 1233,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000938727.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.1859C>T",
          "hgvs_p": "p.Thr620Ile",
          "transcript": "XM_047418130.1",
          "protein_id": "XP_047274086.1",
          "transcript_support_level": null,
          "aa_start": 620,
          "aa_end": null,
          "aa_length": 704,
          "cds_start": 1859,
          "cds_end": null,
          "cds_length": 2115,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047418130.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.1607C>T",
          "hgvs_p": "p.Thr536Ile",
          "transcript": "XM_047418131.1",
          "protein_id": "XP_047274087.1",
          "transcript_support_level": null,
          "aa_start": 536,
          "aa_end": null,
          "aa_length": 620,
          "cds_start": 1607,
          "cds_end": null,
          "cds_length": 1863,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047418131.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": 18,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "c.1936-3659C>T",
          "hgvs_p": null,
          "transcript": "ENST00000884694.1",
          "protein_id": "ENSP00000554753.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 727,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2184,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884694.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEC63",
          "gene_hgnc_id": 21082,
          "hgvs_c": "n.674C>T",
          "hgvs_p": null,
          "transcript": "ENST00000473746.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000473746.1"
        }
      ],
      "gene_symbol": "SEC63",
      "gene_hgnc_id": 21082,
      "dbsnp": "rs61733388",
      "frequency_reference_population": 0.021574927,
      "hom_count_reference_population": 454,
      "allele_count_reference_population": 34042,
      "gnomad_exomes_af": 0.0220302,
      "gnomad_genomes_af": 0.0171538,
      "gnomad_exomes_ac": 31515,
      "gnomad_genomes_ac": 2527,
      "gnomad_exomes_homalt": 427,
      "gnomad_genomes_homalt": 27,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.006633669137954712,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.05999999865889549,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.128,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0958,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.62,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.198,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.06,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_007214.5",
          "gene_symbol": "SEC63",
          "hgnc_id": 21082,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.2027C>T",
          "hgvs_p": "p.Thr676Ile"
        }
      ],
      "clinvar_disease": "Polycystic liver disease 2,not provided,not specified",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:2 B:3",
      "phenotype_combined": "Polycystic liver disease 2|not specified|not provided",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}
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