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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-108356698-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=108356698&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 108356698,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_145315.5",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.526C>A",
          "hgvs_p": "p.Arg176Ser",
          "transcript": "NM_145315.5",
          "protein_id": "NP_660358.2",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 481,
          "cds_start": 526,
          "cds_end": null,
          "cds_length": 1446,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000368977.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_145315.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.526C>A",
          "hgvs_p": "p.Arg176Ser",
          "transcript": "ENST00000368977.9",
          "protein_id": "ENSP00000357973.3",
          "transcript_support_level": 1,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 481,
          "cds_start": 526,
          "cds_end": null,
          "cds_length": 1446,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_145315.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000368977.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.577C>A",
          "hgvs_p": "p.Arg193Ser",
          "transcript": "ENST00000908138.1",
          "protein_id": "ENSP00000578197.1",
          "transcript_support_level": null,
          "aa_start": 193,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 577,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000908138.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.520C>A",
          "hgvs_p": "p.Arg174Ser",
          "transcript": "ENST00000908137.1",
          "protein_id": "ENSP00000578196.1",
          "transcript_support_level": null,
          "aa_start": 174,
          "aa_end": null,
          "aa_length": 479,
          "cds_start": 520,
          "cds_end": null,
          "cds_length": 1440,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000908137.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.526C>A",
          "hgvs_p": "p.Arg176Ser",
          "transcript": "ENST00000955934.1",
          "protein_id": "ENSP00000625993.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 471,
          "cds_start": 526,
          "cds_end": null,
          "cds_length": 1416,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955934.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.424C>A",
          "hgvs_p": "p.Arg142Ser",
          "transcript": "ENST00000908136.1",
          "protein_id": "ENSP00000578195.1",
          "transcript_support_level": null,
          "aa_start": 142,
          "aa_end": null,
          "aa_length": 447,
          "cds_start": 424,
          "cds_end": null,
          "cds_length": 1344,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000908136.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.526C>A",
          "hgvs_p": "p.Arg176Ser",
          "transcript": "NM_001323005.2",
          "protein_id": "NP_001309934.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 526,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001323005.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.526C>A",
          "hgvs_p": "p.Arg176Ser",
          "transcript": "ENST00000908142.1",
          "protein_id": "ENSP00000578201.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 526,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000908142.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.520C>A",
          "hgvs_p": "p.Arg174Ser",
          "transcript": "ENST00000908139.1",
          "protein_id": "ENSP00000578198.1",
          "transcript_support_level": null,
          "aa_start": 174,
          "aa_end": null,
          "aa_length": 432,
          "cds_start": 520,
          "cds_end": null,
          "cds_length": 1299,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000908139.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.127C>A",
          "hgvs_p": "p.Arg43Ser",
          "transcript": "ENST00000421954.5",
          "protein_id": "ENSP00000398225.1",
          "transcript_support_level": 5,
          "aa_start": 43,
          "aa_end": null,
          "aa_length": 348,
          "cds_start": 127,
          "cds_end": null,
          "cds_length": 1047,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000421954.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.427C>A",
          "hgvs_p": "p.Arg143Ser",
          "transcript": "ENST00000437715.1",
          "protein_id": "ENSP00000392085.1",
          "transcript_support_level": 5,
          "aa_start": 143,
          "aa_end": null,
          "aa_length": 159,
          "cds_start": 427,
          "cds_end": null,
          "cds_length": 480,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000437715.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.427C>A",
          "hgvs_p": "p.Arg143Ser",
          "transcript": "XM_005266885.4",
          "protein_id": "XP_005266942.1",
          "transcript_support_level": null,
          "aa_start": 143,
          "aa_end": null,
          "aa_length": 448,
          "cds_start": 427,
          "cds_end": null,
          "cds_length": 1347,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005266885.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.424C>A",
          "hgvs_p": "p.Arg142Ser",
          "transcript": "XM_011535657.3",
          "protein_id": "XP_011533959.1",
          "transcript_support_level": null,
          "aa_start": 142,
          "aa_end": null,
          "aa_length": 447,
          "cds_start": 424,
          "cds_end": null,
          "cds_length": 1344,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535657.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.418C>A",
          "hgvs_p": "p.Arg140Ser",
          "transcript": "XM_047418557.1",
          "protein_id": "XP_047274513.1",
          "transcript_support_level": null,
          "aa_start": 140,
          "aa_end": null,
          "aa_length": 445,
          "cds_start": 418,
          "cds_end": null,
          "cds_length": 1338,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047418557.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.97C>A",
          "hgvs_p": "p.Arg33Ser",
          "transcript": "XM_017010648.2",
          "protein_id": "XP_016866137.1",
          "transcript_support_level": null,
          "aa_start": 33,
          "aa_end": null,
          "aa_length": 338,
          "cds_start": 97,
          "cds_end": null,
          "cds_length": 1017,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017010648.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.97C>A",
          "hgvs_p": "p.Arg33Ser",
          "transcript": "XM_047418558.1",
          "protein_id": "XP_047274514.1",
          "transcript_support_level": null,
          "aa_start": 33,
          "aa_end": null,
          "aa_length": 338,
          "cds_start": 97,
          "cds_end": null,
          "cds_length": 1017,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_047418558.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.526C>A",
          "hgvs_p": "p.Arg176Ser",
          "transcript": "XM_011535659.4",
          "protein_id": "XP_011533961.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 281,
          "cds_start": 526,
          "cds_end": null,
          "cds_length": 846,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
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          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.526C>A",
          "hgvs_p": "p.Arg176Ser",
          "transcript": "XM_011535660.3",
          "protein_id": "XP_011533962.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 279,
          "cds_start": 526,
          "cds_end": null,
          "cds_length": 840,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_011535660.3"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
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          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.526C>A",
          "hgvs_p": "p.Arg176Ser",
          "transcript": "XM_011535661.3",
          "protein_id": "XP_011533963.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 254,
          "cds_start": 526,
          "cds_end": null,
          "cds_length": 765,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535661.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "c.-39C>A",
          "hgvs_p": null,
          "transcript": "XM_047418559.1",
          "protein_id": "XP_047274515.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 293,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 882,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047418559.1"
        },
        {
          "aa_ref": null,
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          "canonical": false,
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          "cds_length": 837,
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        },
        {
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          "protein_coding": false,
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          "consequences": [
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          ],
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "AFG1L",
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          "hgvs_c": "n.7C>A",
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          "transcript": "ENST00000431865.1",
          "protein_id": "ENSP00000415484.1",
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          "cds_start": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000431865.1"
        },
        {
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
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          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "AFG1L",
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          "hgvs_c": "n.174C>A",
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          "transcript": "NR_136553.2",
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          "cdna_start": null,
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          "biotype": "pseudogene",
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        },
        {
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          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
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          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "AFG1L",
          "gene_hgnc_id": 16411,
          "hgvs_c": "n.552C>A",
          "hgvs_p": null,
          "transcript": "XR_007059229.1",
          "protein_id": null,
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          "aa_length": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_007059229.1"
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      ],
      "gene_symbol": "AFG1L",
      "gene_hgnc_id": 16411,
      "dbsnp": "rs1337014099",
      "frequency_reference_population": 0.0000020702305,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000207023,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.2839518189430237,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.07000000029802322,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.108,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.3893,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.24,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.521,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.07,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_145315.5",
          "gene_symbol": "AFG1L",
          "hgnc_id": 16411,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.526C>A",
          "hgvs_p": "p.Arg176Ser"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}