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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-116545474-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=116545474&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 116545474,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "NM_001139444.3",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAPPC3L",
          "gene_hgnc_id": 21090,
          "hgvs_c": "c.41T>C",
          "hgvs_p": "p.Ile14Thr",
          "transcript": "NM_001139444.3",
          "protein_id": "NP_001132916.1",
          "transcript_support_level": null,
          "aa_start": 14,
          "aa_end": null,
          "aa_length": 181,
          "cds_start": 41,
          "cds_end": null,
          "cds_length": 546,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000368602.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001139444.3"
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAPPC3L",
          "gene_hgnc_id": 21090,
          "hgvs_c": "c.41T>C",
          "hgvs_p": "p.Ile14Thr",
          "transcript": "ENST00000368602.4",
          "protein_id": "ENSP00000357591.3",
          "transcript_support_level": 5,
          "aa_start": 14,
          "aa_end": null,
          "aa_length": 181,
          "cds_start": 41,
          "cds_end": null,
          "cds_length": 546,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001139444.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000368602.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.-33+1602A>G",
          "hgvs_p": null,
          "transcript": "ENST00000405399.5",
          "protein_id": "ENSP00000385836.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 171,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 516,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000405399.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.-1+1602A>G",
          "hgvs_p": null,
          "transcript": "ENST00000368597.6",
          "protein_id": "ENSP00000357586.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 128,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 387,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000368597.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.-33+1602A>G",
          "hgvs_p": null,
          "transcript": "NM_001256887.3",
          "protein_id": "NP_001243816.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 171,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 516,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001256887.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.59+1602A>G",
          "hgvs_p": null,
          "transcript": "NM_001256888.3",
          "protein_id": "NP_001243817.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 170,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 513,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001256888.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.59+1602A>G",
          "hgvs_p": null,
          "transcript": "ENST00000628083.1",
          "protein_id": "ENSP00000487169.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 170,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 513,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000628083.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.-1+1602A>G",
          "hgvs_p": null,
          "transcript": "NM_001256889.3",
          "protein_id": "NP_001243818.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 128,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 387,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001256889.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.-1+1602A>G",
          "hgvs_p": null,
          "transcript": "NM_153036.5",
          "protein_id": "NP_694581.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 128,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 387,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_153036.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.-1+1602A>G",
          "hgvs_p": null,
          "transcript": "ENST00000452373.5",
          "protein_id": "ENSP00000409556.1",
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 122,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 370,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000452373.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.149+1602A>G",
          "hgvs_p": null,
          "transcript": "XM_017010390.2",
          "protein_id": "XP_016865879.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 215,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 648,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017010390.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.149+1602A>G",
          "hgvs_p": null,
          "transcript": "XM_017010391.2",
          "protein_id": "XP_016865880.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 200,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 603,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017010391.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.59+1602A>G",
          "hgvs_p": null,
          "transcript": "XM_011535560.3",
          "protein_id": "XP_011533862.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 185,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 558,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535560.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.59+1602A>G",
          "hgvs_p": null,
          "transcript": "XM_011535561.3",
          "protein_id": "XP_011533863.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 185,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 558,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535561.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.59+1602A>G",
          "hgvs_p": null,
          "transcript": "XM_011535562.3",
          "protein_id": "XP_011533864.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 185,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 558,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535562.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.59+1602A>G",
          "hgvs_p": null,
          "transcript": "XM_011535563.3",
          "protein_id": "XP_011533865.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 185,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 558,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535563.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.59+1602A>G",
          "hgvs_p": null,
          "transcript": "XM_011535564.2",
          "protein_id": "XP_011533866.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 185,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 558,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535564.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "CALHM4",
          "gene_hgnc_id": 21094,
          "hgvs_c": "c.59+1602A>G",
          "hgvs_p": null,
          "transcript": "XM_017010392.2",
          "protein_id": "XP_016865881.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 185,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 558,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017010392.2"
        }
      ],
      "gene_symbol": "TRAPPC3L",
      "gene_hgnc_id": 21090,
      "dbsnp": "rs1355419594",
      "frequency_reference_population": 0.000003234345,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.00000215244,
      "gnomad_genomes_af": 0.000013146,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.5660682916641235,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.7599999904632568,
      "splice_prediction_selected": "Pathogenic",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.353,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.6525,
      "alphamissense_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.34,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 4.188,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.951765438608643,
      "dbscsnv_ada_prediction": "Benign",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001139444.3",
          "gene_symbol": "TRAPPC3L",
          "hgnc_id": 21090,
          "effects": [
            "missense_variant",
            "splice_region_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.41T>C",
          "hgvs_p": "p.Ile14Thr"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001256887.3",
          "gene_symbol": "CALHM4",
          "hgnc_id": 21094,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.-33+1602A>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}