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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-117301025-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=117301025&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 117301025,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_002944.3",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 43,
          "exon_rank_end": null,
          "exon_count": 44,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6664A>C",
          "hgvs_p": "p.Lys2222Gln",
          "transcript": "NM_001378902.1",
          "protein_id": "NP_001365831.1",
          "transcript_support_level": null,
          "aa_start": 2222,
          "aa_end": null,
          "aa_length": 2341,
          "cds_start": 6664,
          "cds_end": null,
          "cds_length": 7026,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000368507.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001378902.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 43,
          "exon_rank_end": null,
          "exon_count": 44,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6664A>C",
          "hgvs_p": "p.Lys2222Gln",
          "transcript": "ENST00000368507.8",
          "protein_id": "ENSP00000357493.3",
          "transcript_support_level": 5,
          "aa_start": 2222,
          "aa_end": null,
          "aa_length": 2341,
          "cds_start": 6664,
          "cds_end": null,
          "cds_length": 7026,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001378902.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000368507.8"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 42,
          "exon_rank_end": null,
          "exon_count": 43,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6682A>C",
          "hgvs_p": "p.Lys2228Gln",
          "transcript": "ENST00000368508.7",
          "protein_id": "ENSP00000357494.3",
          "transcript_support_level": 1,
          "aa_start": 2228,
          "aa_end": null,
          "aa_length": 2347,
          "cds_start": 6682,
          "cds_end": null,
          "cds_length": 7044,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000368508.7"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 43,
          "exon_rank_end": null,
          "exon_count": 44,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6709A>C",
          "hgvs_p": "p.Lys2237Gln",
          "transcript": "ENST00000957000.1",
          "protein_id": "ENSP00000627059.1",
          "transcript_support_level": null,
          "aa_start": 2237,
          "aa_end": null,
          "aa_length": 2356,
          "cds_start": 6709,
          "cds_end": null,
          "cds_length": 7071,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957000.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 42,
          "exon_rank_end": null,
          "exon_count": 43,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6682A>C",
          "hgvs_p": "p.Lys2228Gln",
          "transcript": "NM_002944.3",
          "protein_id": "NP_002935.2",
          "transcript_support_level": null,
          "aa_start": 2228,
          "aa_end": null,
          "aa_length": 2347,
          "cds_start": 6682,
          "cds_end": null,
          "cds_length": 7044,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002944.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 43,
          "exon_rank_end": null,
          "exon_count": 44,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6670A>C",
          "hgvs_p": "p.Lys2224Gln",
          "transcript": "NM_001378891.1",
          "protein_id": "NP_001365820.1",
          "transcript_support_level": null,
          "aa_start": 2224,
          "aa_end": null,
          "aa_length": 2343,
          "cds_start": 6670,
          "cds_end": null,
          "cds_length": 7032,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001378891.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 43,
          "exon_rank_end": null,
          "exon_count": 44,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6535A>C",
          "hgvs_p": "p.Lys2179Gln",
          "transcript": "ENST00000956999.1",
          "protein_id": "ENSP00000627058.1",
          "transcript_support_level": null,
          "aa_start": 2179,
          "aa_end": null,
          "aa_length": 2298,
          "cds_start": 6535,
          "cds_end": null,
          "cds_length": 6897,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956999.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 43,
          "exon_rank_end": null,
          "exon_count": 44,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6712A>C",
          "hgvs_p": "p.Lys2238Gln",
          "transcript": "XM_011536049.3",
          "protein_id": "XP_011534351.1",
          "transcript_support_level": null,
          "aa_start": 2238,
          "aa_end": null,
          "aa_length": 2357,
          "cds_start": 6712,
          "cds_end": null,
          "cds_length": 7074,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011536049.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 43,
          "exon_rank_end": null,
          "exon_count": 44,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6709A>C",
          "hgvs_p": "p.Lys2237Gln",
          "transcript": "XM_011536050.3",
          "protein_id": "XP_011534352.1",
          "transcript_support_level": null,
          "aa_start": 2237,
          "aa_end": null,
          "aa_length": 2356,
          "cds_start": 6709,
          "cds_end": null,
          "cds_length": 7071,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011536050.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 42,
          "exon_rank_end": null,
          "exon_count": 43,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6685A>C",
          "hgvs_p": "p.Lys2229Gln",
          "transcript": "XM_011536051.3",
          "protein_id": "XP_011534353.1",
          "transcript_support_level": null,
          "aa_start": 2229,
          "aa_end": null,
          "aa_length": 2348,
          "cds_start": 6685,
          "cds_end": null,
          "cds_length": 7047,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011536051.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 43,
          "exon_rank_end": null,
          "exon_count": 44,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6667A>C",
          "hgvs_p": "p.Lys2223Gln",
          "transcript": "XM_006715548.5",
          "protein_id": "XP_006715611.1",
          "transcript_support_level": null,
          "aa_start": 2223,
          "aa_end": null,
          "aa_length": 2342,
          "cds_start": 6667,
          "cds_end": null,
          "cds_length": 7029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006715548.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 42,
          "exon_rank_end": null,
          "exon_count": 43,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6643A>C",
          "hgvs_p": "p.Lys2215Gln",
          "transcript": "XM_017011172.2",
          "protein_id": "XP_016866661.1",
          "transcript_support_level": null,
          "aa_start": 2215,
          "aa_end": null,
          "aa_length": 2334,
          "cds_start": 6643,
          "cds_end": null,
          "cds_length": 7005,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017011172.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 42,
          "exon_rank_end": null,
          "exon_count": 43,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6640A>C",
          "hgvs_p": "p.Lys2214Gln",
          "transcript": "XM_017011173.2",
          "protein_id": "XP_016866662.1",
          "transcript_support_level": null,
          "aa_start": 2214,
          "aa_end": null,
          "aa_length": 2333,
          "cds_start": 6640,
          "cds_end": null,
          "cds_length": 7002,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017011173.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 43,
          "exon_rank_end": null,
          "exon_count": 44,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6538A>C",
          "hgvs_p": "p.Lys2180Gln",
          "transcript": "XM_011536053.3",
          "protein_id": "XP_011534355.1",
          "transcript_support_level": null,
          "aa_start": 2180,
          "aa_end": null,
          "aa_length": 2299,
          "cds_start": 6538,
          "cds_end": null,
          "cds_length": 6900,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011536053.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 43,
          "intron_rank": 42,
          "intron_rank_end": null,
          "gene_symbol": "ROS1",
          "gene_hgnc_id": 10261,
          "hgvs_c": "c.6599+7769A>C",
          "hgvs_p": null,
          "transcript": "XM_011536054.3",
          "protein_id": "XP_011534356.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 2200,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 6603,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011536054.3"
        }
      ],
      "gene_symbol": "ROS1",
      "gene_hgnc_id": 10261,
      "dbsnp": "rs529156",
      "frequency_reference_population": 0.23533447,
      "hom_count_reference_population": 47339,
      "allele_count_reference_population": 376040,
      "gnomad_exomes_af": 0.240283,
      "gnomad_genomes_af": 0.1883,
      "gnomad_exomes_ac": 347400,
      "gnomad_genomes_ac": 28640,
      "gnomad_exomes_homalt": 44121,
      "gnomad_genomes_homalt": 3218,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.002709329128265381,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.065,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0607,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.62,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.054,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -14,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Moderate,BA1",
      "acmg_by_gene": [
        {
          "score": -14,
          "benign_score": 14,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Moderate",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "NM_002944.3",
          "gene_symbol": "ROS1",
          "hgnc_id": 10261,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.6682A>C",
          "hgvs_p": "p.Lys2228Gln"
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "B:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}