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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-132815028-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=132815028&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 132815028,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001016.4",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "NM_001016.4",
          "protein_id": "NP_001007.2",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 132,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 399,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000230050.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001016.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000230050.4",
          "protein_id": "ENSP00000230050.3",
          "transcript_support_level": 1,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 132,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 399,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001016.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000230050.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000914103.1",
          "protein_id": "ENSP00000584162.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 220,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 663,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914103.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000914107.1",
          "protein_id": "ENSP00000584166.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 205,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 618,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914107.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000914108.1",
          "protein_id": "ENSP00000584167.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 178,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 537,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914108.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000914114.1",
          "protein_id": "ENSP00000584173.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914114.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000914106.1",
          "protein_id": "ENSP00000584165.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 142,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 429,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914106.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000877751.1",
          "protein_id": "ENSP00000547810.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 132,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 399,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000877751.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000877752.1",
          "protein_id": "ENSP00000547811.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 132,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 399,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000877752.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000914102.1",
          "protein_id": "ENSP00000584161.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 132,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 399,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914102.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000914105.1",
          "protein_id": "ENSP00000584164.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 132,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 399,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914105.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000914101.1",
          "protein_id": "ENSP00000584160.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 127,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 384,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914101.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000914109.1",
          "protein_id": "ENSP00000584168.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 126,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 381,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914109.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000914113.1",
          "protein_id": "ENSP00000584172.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 108,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 327,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914113.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000914104.1",
          "protein_id": "ENSP00000584163.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 98,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 297,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914104.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile",
          "transcript": "ENST00000914111.1",
          "protein_id": "ENSP00000584170.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 82,
          "cds_start": 71,
          "cds_end": null,
          "cds_length": 249,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914111.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.14+246C>T",
          "hgvs_p": null,
          "transcript": "ENST00000877750.1",
          "protein_id": "ENSP00000547809.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 93,
          "cds_start": null,
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          "cds_length": 282,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000877750.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.60+11C>T",
          "hgvs_p": null,
          "transcript": "ENST00000914112.1",
          "protein_id": "ENSP00000584171.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 86,
          "cds_start": null,
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          "cds_length": 261,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914112.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "c.14+246C>T",
          "hgvs_p": null,
          "transcript": "ENST00000914110.1",
          "protein_id": "ENSP00000584169.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 59,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 180,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914110.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS12",
          "gene_hgnc_id": 10385,
          "hgvs_c": "n.153C>T",
          "hgvs_p": null,
          "transcript": "ENST00000484616.2",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000484616.2"
        }
      ],
      "gene_symbol": "RPS12",
      "gene_hgnc_id": 10385,
      "dbsnp": "rs142145207",
      "frequency_reference_population": 0.000016110782,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 26,
      "gnomad_exomes_af": 0.00000821043,
      "gnomad_genomes_af": 0.0000919419,
      "gnomad_exomes_ac": 12,
      "gnomad_genomes_ac": 14,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.5075610280036926,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.336,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.4534,
      "alphamissense_prediction": "Uncertain_significance",
      "bayesdelnoaf_score": 0.17,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.825,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BS2",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001016.4",
          "gene_symbol": "RPS12",
          "hgnc_id": 10385,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.71C>T",
          "hgvs_p": "p.Thr24Ile"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}