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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-138424532-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=138424532&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 138424532,
      "ref": "G",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_020464.2",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4370C>A",
          "hgvs_p": "p.Ser1457Tyr",
          "transcript": "NM_001144060.2",
          "protein_id": "NP_001137532.1",
          "transcript_support_level": null,
          "aa_start": 1457,
          "aa_end": null,
          "aa_length": 1606,
          "cds_start": 4370,
          "cds_end": null,
          "cds_length": 4821,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000343505.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001144060.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4370C>A",
          "hgvs_p": "p.Ser1457Tyr",
          "transcript": "ENST00000343505.10",
          "protein_id": "ENSP00000344672.5",
          "transcript_support_level": 5,
          "aa_start": 1457,
          "aa_end": null,
          "aa_length": 1606,
          "cds_start": 4370,
          "cds_end": null,
          "cds_length": 4821,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001144060.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000343505.10"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4601C>A",
          "hgvs_p": "p.Ser1534Tyr",
          "transcript": "ENST00000491526.7",
          "protein_id": "ENSP00000433523.2",
          "transcript_support_level": 3,
          "aa_start": 1534,
          "aa_end": null,
          "aa_length": 1683,
          "cds_start": 4601,
          "cds_end": null,
          "cds_length": 5052,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000491526.7"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4382C>A",
          "hgvs_p": "p.Ser1461Tyr",
          "transcript": "NM_020464.2",
          "protein_id": "NP_065197.1",
          "transcript_support_level": null,
          "aa_start": 1461,
          "aa_end": null,
          "aa_length": 1610,
          "cds_start": 4382,
          "cds_end": null,
          "cds_length": 4833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_020464.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4382C>A",
          "hgvs_p": "p.Ser1461Tyr",
          "transcript": "ENST00000427025.6",
          "protein_id": "ENSP00000394546.2",
          "transcript_support_level": 5,
          "aa_start": 1461,
          "aa_end": null,
          "aa_length": 1610,
          "cds_start": 4382,
          "cds_end": null,
          "cds_length": 4833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000427025.6"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4655C>A",
          "hgvs_p": "p.Ser1552Tyr",
          "transcript": "XM_011535976.2",
          "protein_id": "XP_011534278.2",
          "transcript_support_level": null,
          "aa_start": 1552,
          "aa_end": null,
          "aa_length": 1701,
          "cds_start": 4655,
          "cds_end": null,
          "cds_length": 5106,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535976.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4601C>A",
          "hgvs_p": "p.Ser1534Tyr",
          "transcript": "XM_047419109.1",
          "protein_id": "XP_047275065.1",
          "transcript_support_level": null,
          "aa_start": 1534,
          "aa_end": null,
          "aa_length": 1683,
          "cds_start": 4601,
          "cds_end": null,
          "cds_length": 5052,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047419109.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4568C>A",
          "hgvs_p": "p.Ser1523Tyr",
          "transcript": "XM_011535966.4",
          "protein_id": "XP_011534268.1",
          "transcript_support_level": null,
          "aa_start": 1523,
          "aa_end": null,
          "aa_length": 1672,
          "cds_start": 4568,
          "cds_end": null,
          "cds_length": 5019,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535966.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4568C>A",
          "hgvs_p": "p.Ser1523Tyr",
          "transcript": "XM_011535967.4",
          "protein_id": "XP_011534269.1",
          "transcript_support_level": null,
          "aa_start": 1523,
          "aa_end": null,
          "aa_length": 1672,
          "cds_start": 4568,
          "cds_end": null,
          "cds_length": 5019,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535967.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4568C>A",
          "hgvs_p": "p.Ser1523Tyr",
          "transcript": "XM_017011086.3",
          "protein_id": "XP_016866575.1",
          "transcript_support_level": null,
          "aa_start": 1523,
          "aa_end": null,
          "aa_length": 1672,
          "cds_start": 4568,
          "cds_end": null,
          "cds_length": 5019,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017011086.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4523C>A",
          "hgvs_p": "p.Ser1508Tyr",
          "transcript": "XM_047419110.1",
          "protein_id": "XP_047275066.1",
          "transcript_support_level": null,
          "aa_start": 1508,
          "aa_end": null,
          "aa_length": 1657,
          "cds_start": 4523,
          "cds_end": null,
          "cds_length": 4974,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047419110.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4514C>A",
          "hgvs_p": "p.Ser1505Tyr",
          "transcript": "XM_047419111.1",
          "protein_id": "XP_047275067.1",
          "transcript_support_level": null,
          "aa_start": 1505,
          "aa_end": null,
          "aa_length": 1654,
          "cds_start": 4514,
          "cds_end": null,
          "cds_length": 4965,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047419111.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4514C>A",
          "hgvs_p": "p.Ser1505Tyr",
          "transcript": "XM_047419112.1",
          "protein_id": "XP_047275068.1",
          "transcript_support_level": null,
          "aa_start": 1505,
          "aa_end": null,
          "aa_length": 1654,
          "cds_start": 4514,
          "cds_end": null,
          "cds_length": 4965,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047419112.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4469C>A",
          "hgvs_p": "p.Ser1490Tyr",
          "transcript": "XM_047419113.1",
          "protein_id": "XP_047275069.1",
          "transcript_support_level": null,
          "aa_start": 1490,
          "aa_end": null,
          "aa_length": 1639,
          "cds_start": 4469,
          "cds_end": null,
          "cds_length": 4920,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047419113.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4463C>A",
          "hgvs_p": "p.Ser1488Tyr",
          "transcript": "XM_017011087.1",
          "protein_id": "XP_016866576.1",
          "transcript_support_level": null,
          "aa_start": 1488,
          "aa_end": null,
          "aa_length": 1637,
          "cds_start": 4463,
          "cds_end": null,
          "cds_length": 4914,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017011087.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4427C>A",
          "hgvs_p": "p.Ser1476Tyr",
          "transcript": "XM_011535969.3",
          "protein_id": "XP_011534271.1",
          "transcript_support_level": null,
          "aa_start": 1476,
          "aa_end": null,
          "aa_length": 1625,
          "cds_start": 4427,
          "cds_end": null,
          "cds_length": 4878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535969.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4424C>A",
          "hgvs_p": "p.Ser1475Tyr",
          "transcript": "XM_011535970.3",
          "protein_id": "XP_011534272.1",
          "transcript_support_level": null,
          "aa_start": 1475,
          "aa_end": null,
          "aa_length": 1624,
          "cds_start": 4424,
          "cds_end": null,
          "cds_length": 4875,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011535970.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4418C>A",
          "hgvs_p": "p.Ser1473Tyr",
          "transcript": "XM_017011088.2",
          "protein_id": "XP_016866577.1",
          "transcript_support_level": null,
          "aa_start": 1473,
          "aa_end": null,
          "aa_length": 1622,
          "cds_start": 4418,
          "cds_end": null,
          "cds_length": 4869,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017011088.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4409C>A",
          "hgvs_p": "p.Ser1470Tyr",
          "transcript": "XM_047419114.1",
          "protein_id": "XP_047275070.1",
          "transcript_support_level": null,
          "aa_start": 1470,
          "aa_end": null,
          "aa_length": 1619,
          "cds_start": 4409,
          "cds_end": null,
          "cds_length": 4860,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047419114.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NHSL1",
          "gene_hgnc_id": 21021,
          "hgvs_c": "c.4406C>A",
          "hgvs_p": "p.Ser1469Tyr",
          "transcript": "XM_011535971.3",
          "protein_id": "XP_011534273.1",
          "transcript_support_level": null,
          "aa_start": 1469,
          "aa_end": null,
          "aa_length": 1618,
          "cds_start": 4406,
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      "dbsnp": "rs369507715",
      "frequency_reference_population": 0.000004511263,
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      "gnomad_exomes_af": 0.00000357296,
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      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.14413198828697205,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.165,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1656,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.48,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.53,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_020464.2",
          "gene_symbol": "NHSL1",
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          "effects": [
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          ],
          "inheritance_mode": "AR",
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          "hgvs_p": "p.Ser1461Tyr"
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}