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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-146399414-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=146399414&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 146399414,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000282753.6",
      "consequences": [
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "NM_001278064.2",
          "protein_id": "NP_001264993.1",
          "transcript_support_level": null,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 1194,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 3585,
          "cdna_start": 2831,
          "cdna_end": null,
          "cdna_length": 6846,
          "mane_select": "ENST00000282753.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "ENST00000282753.6",
          "protein_id": "ENSP00000282753.1",
          "transcript_support_level": 1,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 1194,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 3585,
          "cdna_start": 2831,
          "cdna_end": null,
          "cdna_length": 6846,
          "mane_select": "NM_001278064.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "ENST00000355289.8",
          "protein_id": "ENSP00000347437.4",
          "transcript_support_level": 1,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 908,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 2727,
          "cdna_start": 2610,
          "cdna_end": null,
          "cdna_length": 3874,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "ENST00000492807.6",
          "protein_id": "ENSP00000424095.1",
          "transcript_support_level": 1,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 906,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 2721,
          "cdna_start": 2742,
          "cdna_end": null,
          "cdna_length": 6836,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "ENST00000507907.1",
          "protein_id": "ENSP00000425599.1",
          "transcript_support_level": 1,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 906,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 2721,
          "cdna_start": 2407,
          "cdna_end": null,
          "cdna_length": 3756,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "ENST00000361719.6",
          "protein_id": "ENSP00000354896.2",
          "transcript_support_level": 5,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 1194,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 3585,
          "cdna_start": 2742,
          "cdna_end": null,
          "cdna_length": 6754,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "ENST00000706833.1",
          "protein_id": "ENSP00000516579.1",
          "transcript_support_level": null,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 1194,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 3585,
          "cdna_start": 2823,
          "cdna_end": null,
          "cdna_length": 5597,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "ENST00000706835.1",
          "protein_id": "ENSP00000516581.1",
          "transcript_support_level": null,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 1194,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 3585,
          "cdna_start": 2781,
          "cdna_end": null,
          "cdna_length": 5423,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "NM_001278067.1",
          "protein_id": "NP_001264996.1",
          "transcript_support_level": null,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 908,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 2727,
          "cdna_start": 2695,
          "cdna_end": null,
          "cdna_length": 6672,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "NM_001278065.2",
          "protein_id": "NP_001264994.1",
          "transcript_support_level": null,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 906,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 2721,
          "cdna_start": 2817,
          "cdna_end": null,
          "cdna_length": 6917,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "NM_001278066.1",
          "protein_id": "NP_001264995.1",
          "transcript_support_level": null,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 906,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 2721,
          "cdna_start": 2695,
          "cdna_end": null,
          "cdna_length": 6757,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "ENST00000706834.1",
          "protein_id": "ENSP00000516580.1",
          "transcript_support_level": null,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 906,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 2721,
          "cdna_start": 2781,
          "cdna_end": null,
          "cdna_length": 5329,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "ENST00000706836.1",
          "protein_id": "ENSP00000516582.1",
          "transcript_support_level": null,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 906,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 2721,
          "cdna_start": 2781,
          "cdna_end": null,
          "cdna_length": 6881,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "XM_011535782.2",
          "protein_id": "XP_011534084.1",
          "transcript_support_level": null,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 1194,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 3585,
          "cdna_start": 2784,
          "cdna_end": null,
          "cdna_length": 6799,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "XM_017010783.2",
          "protein_id": "XP_016866272.1",
          "transcript_support_level": null,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 1194,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 3585,
          "cdna_start": 2781,
          "cdna_end": null,
          "cdna_length": 6796,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys",
          "transcript": "XM_017010784.2",
          "protein_id": "XP_016866273.1",
          "transcript_support_level": null,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 1194,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 3585,
          "cdna_start": 2817,
          "cdna_end": null,
          "cdna_length": 6832,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRM1",
          "gene_hgnc_id": 4593,
          "hgvs_c": "c.1148A>G",
          "hgvs_p": "p.Tyr383Cys",
          "transcript": "XM_017010788.2",
          "protein_id": "XP_016866277.1",
          "transcript_support_level": null,
          "aa_start": 383,
          "aa_end": null,
          "aa_length": 785,
          "cds_start": 1148,
          "cds_end": null,
          "cds_length": 2358,
          "cdna_start": 1565,
          "cdna_end": null,
          "cdna_length": 5580,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "GRM1",
      "gene_hgnc_id": 4593,
      "dbsnp": "rs1554308513",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9100942015647888,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.912,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9967,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.44,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.325,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Moderate,PP5_Moderate",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM2",
            "PP3_Moderate",
            "PP5_Moderate"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000282753.6",
          "gene_symbol": "GRM1",
          "hgnc_id": 4593,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.2375A>G",
          "hgvs_p": "p.Tyr792Cys"
        }
      ],
      "clinvar_disease": "Spinocerebellar ataxia 44,not provided",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LP:1",
      "phenotype_combined": "Spinocerebellar ataxia 44|not provided",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}