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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-158784657-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=158784657&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 158784657,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_003379.5",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "NM_001111077.2",
          "protein_id": "NP_001104547.1",
          "transcript_support_level": null,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 1761,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000367075.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001111077.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "ENST00000367075.4",
          "protein_id": "ENSP00000356042.3",
          "transcript_support_level": 1,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 1761,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001111077.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000367075.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "ENST00000337147.11",
          "protein_id": "ENSP00000338934.7",
          "transcript_support_level": 1,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 1761,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000337147.11"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.676C>T",
          "hgvs_p": "p.Arg226Cys",
          "transcript": "ENST00000852607.1",
          "protein_id": "ENSP00000522666.1",
          "transcript_support_level": null,
          "aa_start": 226,
          "aa_end": null,
          "aa_length": 632,
          "cds_start": 676,
          "cds_end": null,
          "cds_length": 1899,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852607.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.676C>T",
          "hgvs_p": "p.Arg226Cys",
          "transcript": "ENST00000852609.1",
          "protein_id": "ENSP00000522668.1",
          "transcript_support_level": null,
          "aa_start": 226,
          "aa_end": null,
          "aa_length": 632,
          "cds_start": 676,
          "cds_end": null,
          "cds_length": 1899,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852609.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "ENST00000852613.1",
          "protein_id": "ENSP00000522672.1",
          "transcript_support_level": null,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 611,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 1836,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852613.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "ENST00000923503.1",
          "protein_id": "ENSP00000593562.1",
          "transcript_support_level": null,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 611,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 1836,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923503.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "ENST00000945664.1",
          "protein_id": "ENSP00000615723.1",
          "transcript_support_level": null,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 599,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 1800,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945664.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "NM_003379.5",
          "protein_id": "NP_003370.2",
          "transcript_support_level": null,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 1761,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003379.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "ENST00000852605.1",
          "protein_id": "ENSP00000522664.1",
          "transcript_support_level": null,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 1761,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852605.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "ENST00000852606.1",
          "protein_id": "ENSP00000522665.1",
          "transcript_support_level": null,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 1761,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852606.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "ENST00000923500.1",
          "protein_id": "ENSP00000593559.1",
          "transcript_support_level": null,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 1761,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923500.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "ENST00000923502.1",
          "protein_id": "ENSP00000593561.1",
          "transcript_support_level": null,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 1761,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923502.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.529C>T",
          "hgvs_p": "p.Arg177Cys",
          "transcript": "ENST00000852603.1",
          "protein_id": "ENSP00000522662.1",
          "transcript_support_level": null,
          "aa_start": 177,
          "aa_end": null,
          "aa_length": 583,
          "cds_start": 529,
          "cds_end": null,
          "cds_length": 1752,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852603.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.454C>T",
          "hgvs_p": "p.Arg152Cys",
          "transcript": "ENST00000852614.1",
          "protein_id": "ENSP00000522673.1",
          "transcript_support_level": null,
          "aa_start": 152,
          "aa_end": null,
          "aa_length": 558,
          "cds_start": 454,
          "cds_end": null,
          "cds_length": 1677,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852614.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.454C>T",
          "hgvs_p": "p.Arg152Cys",
          "transcript": "ENST00000923506.1",
          "protein_id": "ENSP00000593565.1",
          "transcript_support_level": null,
          "aa_start": 152,
          "aa_end": null,
          "aa_length": 558,
          "cds_start": 454,
          "cds_end": null,
          "cds_length": 1677,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923506.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.442C>T",
          "hgvs_p": "p.Arg148Cys",
          "transcript": "ENST00000852604.1",
          "protein_id": "ENSP00000522663.1",
          "transcript_support_level": null,
          "aa_start": 148,
          "aa_end": null,
          "aa_length": 554,
          "cds_start": 442,
          "cds_end": null,
          "cds_length": 1665,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000852604.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.442C>T",
          "hgvs_p": "p.Arg148Cys",
          "transcript": "ENST00000852610.1",
          "protein_id": "ENSP00000522669.1",
          "transcript_support_level": null,
          "aa_start": 148,
          "aa_end": null,
          "aa_length": 554,
          "cds_start": 442,
          "cds_end": null,
          "cds_length": 1665,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852610.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
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          "consequences": [
            "missense_variant"
          ],
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          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "ENST00000852612.1",
          "protein_id": "ENSP00000522671.1",
          "transcript_support_level": null,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 538,
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          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852612.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EZR",
          "gene_hgnc_id": 12691,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "ENST00000945663.1",
          "protein_id": "ENSP00000615722.1",
          "transcript_support_level": null,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        {
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          "biotype": "pseudogene",
          "feature": "ENST00000476189.1"
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      ],
      "gene_symbol": "EZR",
      "gene_hgnc_id": 12691,
      "dbsnp": "rs3103004",
      "frequency_reference_population": 0.00049752847,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 803,
      "gnomad_exomes_af": 0.000495279,
      "gnomad_genomes_af": 0.000519136,
      "gnomad_exomes_ac": 724,
      "gnomad_genomes_ac": 79,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.22374552488327026,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.15000000596046448,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.383,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.0987,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.17,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.236,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.15,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_003379.5",
          "gene_symbol": "EZR",
          "hgnc_id": 12691,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}