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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-159777328-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=159777328&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 159777328,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001303253.1",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.784G>A",
          "hgvs_p": "p.Val262Ile",
          "transcript": "NM_005891.3",
          "protein_id": "NP_005882.2",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 397,
          "cds_start": 784,
          "cds_end": null,
          "cds_length": 1194,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000367048.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005891.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.784G>A",
          "hgvs_p": "p.Val262Ile",
          "transcript": "ENST00000367048.5",
          "protein_id": "ENSP00000356015.4",
          "transcript_support_level": 1,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 397,
          "cds_start": 784,
          "cds_end": null,
          "cds_length": 1194,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_005891.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000367048.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.871G>A",
          "hgvs_p": "p.Val291Ile",
          "transcript": "NM_001303253.1",
          "protein_id": "NP_001290182.1",
          "transcript_support_level": null,
          "aa_start": 291,
          "aa_end": null,
          "aa_length": 426,
          "cds_start": 871,
          "cds_end": null,
          "cds_length": 1281,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001303253.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.811G>A",
          "hgvs_p": "p.Val271Ile",
          "transcript": "ENST00000869581.1",
          "protein_id": "ENSP00000539640.1",
          "transcript_support_level": null,
          "aa_start": 271,
          "aa_end": null,
          "aa_length": 406,
          "cds_start": 811,
          "cds_end": null,
          "cds_length": 1221,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000869581.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.808G>A",
          "hgvs_p": "p.Val270Ile",
          "transcript": "ENST00000869587.1",
          "protein_id": "ENSP00000539646.1",
          "transcript_support_level": null,
          "aa_start": 270,
          "aa_end": null,
          "aa_length": 405,
          "cds_start": 808,
          "cds_end": null,
          "cds_length": 1218,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000869587.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.790G>A",
          "hgvs_p": "p.Val264Ile",
          "transcript": "ENST00000934474.1",
          "protein_id": "ENSP00000604533.1",
          "transcript_support_level": null,
          "aa_start": 264,
          "aa_end": null,
          "aa_length": 399,
          "cds_start": 790,
          "cds_end": null,
          "cds_length": 1200,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000934474.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.775G>A",
          "hgvs_p": "p.Val259Ile",
          "transcript": "ENST00000934470.1",
          "protein_id": "ENSP00000604529.1",
          "transcript_support_level": null,
          "aa_start": 259,
          "aa_end": null,
          "aa_length": 394,
          "cds_start": 775,
          "cds_end": null,
          "cds_length": 1185,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000934470.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.772G>A",
          "hgvs_p": "p.Val258Ile",
          "transcript": "ENST00000869590.1",
          "protein_id": "ENSP00000539649.1",
          "transcript_support_level": null,
          "aa_start": 258,
          "aa_end": null,
          "aa_length": 393,
          "cds_start": 772,
          "cds_end": null,
          "cds_length": 1182,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000869590.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.763G>A",
          "hgvs_p": "p.Val255Ile",
          "transcript": "ENST00000869582.1",
          "protein_id": "ENSP00000539641.1",
          "transcript_support_level": null,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 390,
          "cds_start": 763,
          "cds_end": null,
          "cds_length": 1173,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000869582.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.757G>A",
          "hgvs_p": "p.Val253Ile",
          "transcript": "ENST00000934473.1",
          "protein_id": "ENSP00000604532.1",
          "transcript_support_level": null,
          "aa_start": 253,
          "aa_end": null,
          "aa_length": 388,
          "cds_start": 757,
          "cds_end": null,
          "cds_length": 1167,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000934473.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.727G>A",
          "hgvs_p": "p.Val243Ile",
          "transcript": "ENST00000934471.1",
          "protein_id": "ENSP00000604530.1",
          "transcript_support_level": null,
          "aa_start": 243,
          "aa_end": null,
          "aa_length": 378,
          "cds_start": 727,
          "cds_end": null,
          "cds_length": 1137,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000934471.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.721G>A",
          "hgvs_p": "p.Val241Ile",
          "transcript": "ENST00000869584.1",
          "protein_id": "ENSP00000539643.1",
          "transcript_support_level": null,
          "aa_start": 241,
          "aa_end": null,
          "aa_length": 376,
          "cds_start": 721,
          "cds_end": null,
          "cds_length": 1131,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000869584.1"
        },
        {
          "aa_ref": "V",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.706G>A",
          "hgvs_p": "p.Val236Ile",
          "transcript": "ENST00000869585.1",
          "protein_id": "ENSP00000539644.1",
          "transcript_support_level": null,
          "aa_start": 236,
          "aa_end": null,
          "aa_length": 371,
          "cds_start": 706,
          "cds_end": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.784G>A",
          "hgvs_p": "p.Val262Ile",
          "transcript": "ENST00000869586.1",
          "protein_id": "ENSP00000539645.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 360,
          "cds_start": 784,
          "cds_end": null,
          "cds_length": 1083,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000869586.1"
        },
        {
          "aa_ref": "V",
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          "protein_coding": true,
          "strand": true,
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          ],
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.640G>A",
          "hgvs_p": "p.Val214Ile",
          "transcript": "ENST00000869591.1",
          "protein_id": "ENSP00000539650.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 349,
          "cds_start": 640,
          "cds_end": null,
          "cds_length": 1050,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000869591.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.517G>A",
          "hgvs_p": "p.Val173Ile",
          "transcript": "ENST00000869588.1",
          "protein_id": "ENSP00000539647.1",
          "transcript_support_level": null,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 308,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 927,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000869588.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
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          ],
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          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.511G>A",
          "hgvs_p": "p.Val171Ile",
          "transcript": "ENST00000869589.1",
          "protein_id": "ENSP00000539648.1",
          "transcript_support_level": null,
          "aa_start": 171,
          "aa_end": null,
          "aa_length": 306,
          "cds_start": 511,
          "cds_end": null,
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        },
        {
          "aa_ref": "V",
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          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.484G>A",
          "hgvs_p": "p.Val162Ile",
          "transcript": "ENST00000869583.1",
          "protein_id": "ENSP00000539642.1",
          "transcript_support_level": null,
          "aa_start": 162,
          "aa_end": null,
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          "cds_start": 484,
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          "cds_length": 894,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000869583.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
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          "strand": true,
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          ],
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          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.484G>A",
          "hgvs_p": "p.Val162Ile",
          "transcript": "ENST00000942453.1",
          "protein_id": "ENSP00000612512.1",
          "transcript_support_level": null,
          "aa_start": 162,
          "aa_end": null,
          "aa_length": 260,
          "cds_start": 484,
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          "cds_length": 783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942453.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "c.340G>A",
          "hgvs_p": "p.Val114Ile",
          "transcript": "ENST00000934472.1",
          "protein_id": "ENSP00000604531.1",
          "transcript_support_level": null,
          "aa_start": 114,
          "aa_end": null,
          "aa_length": 249,
          "cds_start": 340,
          "cds_end": null,
          "cds_length": 750,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000934472.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACAT2",
          "gene_hgnc_id": 94,
          "hgvs_c": "n.1014G>A",
          "hgvs_p": null,
          "transcript": "ENST00000472052.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000472052.1"
        }
      ],
      "gene_symbol": "ACAT2",
      "gene_hgnc_id": 94,
      "dbsnp": "rs759175309",
      "frequency_reference_population": 0.000101017235,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 163,
      "gnomad_exomes_af": 0.00010401,
      "gnomad_genomes_af": 0.0000722762,
      "gnomad_exomes_ac": 152,
      "gnomad_genomes_ac": 11,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.1898062825202942,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.415,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.0932,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.21,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.364,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001303253.1",
          "gene_symbol": "ACAT2",
          "hgnc_id": 94,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,Unknown",
          "hgvs_c": "c.871G>A",
          "hgvs_p": "p.Val291Ile"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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