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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-166412777-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=166412777&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 166412777,
      "ref": "C",
      "alt": "T",
      "effect": "synonymous_variant",
      "transcript": "NM_001318936.2",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.2187G>A",
          "hgvs_p": "p.Thr729Thr",
          "transcript": "NM_021135.6",
          "protein_id": "NP_066958.2",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 733,
          "cds_start": 2187,
          "cds_end": null,
          "cds_length": 2202,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000265678.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_021135.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.2187G>A",
          "hgvs_p": "p.Thr729Thr",
          "transcript": "ENST00000265678.9",
          "protein_id": "ENSP00000265678.4",
          "transcript_support_level": 1,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 733,
          "cds_start": 2187,
          "cds_end": null,
          "cds_length": 2202,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_021135.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000265678.9"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1920G>A",
          "hgvs_p": "p.Thr640Thr",
          "transcript": "ENST00000481261.6",
          "protein_id": "ENSP00000422484.1",
          "transcript_support_level": 1,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 644,
          "cds_start": 1920,
          "cds_end": null,
          "cds_length": 1935,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000481261.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "n.723G>A",
          "hgvs_p": null,
          "transcript": "ENST00000509742.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000509742.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.2262G>A",
          "hgvs_p": "p.Thr754Thr",
          "transcript": "NM_001318936.2",
          "protein_id": "NP_001305865.2",
          "transcript_support_level": null,
          "aa_start": 754,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 2262,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318936.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.2262G>A",
          "hgvs_p": "p.Thr754Thr",
          "transcript": "ENST00000510118.5",
          "protein_id": "ENSP00000422435.1",
          "transcript_support_level": 2,
          "aa_start": 754,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 2262,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000510118.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.2262G>A",
          "hgvs_p": "p.Thr754Thr",
          "transcript": "ENST00000967274.1",
          "protein_id": "ENSP00000637333.1",
          "transcript_support_level": null,
          "aa_start": 754,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 2262,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967274.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.2211G>A",
          "hgvs_p": "p.Thr737Thr",
          "transcript": "NM_001006932.3",
          "protein_id": "NP_001006933.3",
          "transcript_support_level": null,
          "aa_start": 737,
          "aa_end": null,
          "aa_length": 741,
          "cds_start": 2211,
          "cds_end": null,
          "cds_length": 2226,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001006932.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.2211G>A",
          "hgvs_p": "p.Thr737Thr",
          "transcript": "ENST00000503859.5",
          "protein_id": "ENSP00000427015.1",
          "transcript_support_level": 2,
          "aa_start": 737,
          "aa_end": null,
          "aa_length": 741,
          "cds_start": 2211,
          "cds_end": null,
          "cds_length": 2226,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000503859.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.2154G>A",
          "hgvs_p": "p.Thr718Thr",
          "transcript": "ENST00000907318.1",
          "protein_id": "ENSP00000577377.1",
          "transcript_support_level": null,
          "aa_start": 718,
          "aa_end": null,
          "aa_length": 722,
          "cds_start": 2154,
          "cds_end": null,
          "cds_length": 2169,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000907318.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.2139G>A",
          "hgvs_p": "p.Thr713Thr",
          "transcript": "ENST00000714395.1",
          "protein_id": "ENSP00000519662.1",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 717,
          "cds_start": 2139,
          "cds_end": null,
          "cds_length": 2154,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000714395.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.2097G>A",
          "hgvs_p": "p.Thr699Thr",
          "transcript": "ENST00000907315.1",
          "protein_id": "ENSP00000577374.1",
          "transcript_support_level": null,
          "aa_start": 699,
          "aa_end": null,
          "aa_length": 703,
          "cds_start": 2097,
          "cds_end": null,
          "cds_length": 2112,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000907315.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.2067G>A",
          "hgvs_p": "p.Thr689Thr",
          "transcript": "ENST00000907314.1",
          "protein_id": "ENSP00000577373.1",
          "transcript_support_level": null,
          "aa_start": 689,
          "aa_end": null,
          "aa_length": 693,
          "cds_start": 2067,
          "cds_end": null,
          "cds_length": 2082,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000907314.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.2025G>A",
          "hgvs_p": "p.Thr675Thr",
          "transcript": "ENST00000935351.1",
          "protein_id": "ENSP00000605410.1",
          "transcript_support_level": null,
          "aa_start": 675,
          "aa_end": null,
          "aa_length": 679,
          "cds_start": 2025,
          "cds_end": null,
          "cds_length": 2040,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935351.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1992G>A",
          "hgvs_p": "p.Thr664Thr",
          "transcript": "ENST00000907313.1",
          "protein_id": "ENSP00000577372.1",
          "transcript_support_level": null,
          "aa_start": 664,
          "aa_end": null,
          "aa_length": 668,
          "cds_start": 1992,
          "cds_end": null,
          "cds_length": 2007,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000907313.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1980G>A",
          "hgvs_p": "p.Thr660Thr",
          "transcript": "ENST00000907317.1",
          "protein_id": "ENSP00000577376.1",
          "transcript_support_level": null,
          "aa_start": 660,
          "aa_end": null,
          "aa_length": 664,
          "cds_start": 1980,
          "cds_end": null,
          "cds_length": 1995,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000907317.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1938G>A",
          "hgvs_p": "p.Thr646Thr",
          "transcript": "ENST00000907316.1",
          "protein_id": "ENSP00000577375.1",
          "transcript_support_level": null,
          "aa_start": 646,
          "aa_end": null,
          "aa_length": 650,
          "cds_start": 1938,
          "cds_end": null,
          "cds_length": 1953,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000907316.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1920G>A",
          "hgvs_p": "p.Thr640Thr",
          "transcript": "NM_001318938.1",
          "protein_id": "NP_001305867.1",
          "transcript_support_level": null,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 644,
          "cds_start": 1920,
          "cds_end": null,
          "cds_length": 1935,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318938.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1920G>A",
          "hgvs_p": "p.Thr640Thr",
          "transcript": "ENST00000405189.7",
          "protein_id": "ENSP00000386050.3",
          "transcript_support_level": 2,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 644,
          "cds_start": 1920,
          "cds_end": null,
          "cds_length": 1935,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000405189.7"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1893G>A",
          "hgvs_p": "p.Thr631Thr",
          "transcript": "NM_001318937.2",
          "protein_id": "NP_001305866.1",
          "transcript_support_level": null,
          "aa_start": 631,
          "aa_end": null,
          "aa_length": 635,
          "cds_start": 1893,
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          "cds_length": 1908,
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        {
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          ],
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        {
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          "biotype": "pseudogene",
          "feature": "XR_007059866.1"
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      ],
      "gene_symbol": "RPS6KA2",
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      "dbsnp": "rs34651286",
      "frequency_reference_population": 6.9137263e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.91373e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.4099999964237213,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.41,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.793,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -1,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Moderate,BP7",
      "acmg_by_gene": [
        {
          "score": -1,
          "benign_score": 3,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate",
            "BP7"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001318936.2",
          "gene_symbol": "RPS6KA2",
          "hgnc_id": 10431,
          "effects": [
            "synonymous_variant"
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          "inheritance_mode": "AD",
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        {
          "score": 0,
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          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "XR_007059866.1",
          "gene_symbol": "LOC124901460",
          "hgnc_id": null,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.-109C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}