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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-166432418-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=166432418&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 166432418,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001318936.2",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1405A>G",
          "hgvs_p": "p.Ile469Val",
          "transcript": "NM_021135.6",
          "protein_id": "NP_066958.2",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 733,
          "cds_start": 1405,
          "cds_end": null,
          "cds_length": 2202,
          "cdna_start": 1637,
          "cdna_end": null,
          "cdna_length": 5832,
          "mane_select": "ENST00000265678.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_021135.6"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1405A>G",
          "hgvs_p": "p.Ile469Val",
          "transcript": "ENST00000265678.9",
          "protein_id": "ENSP00000265678.4",
          "transcript_support_level": 1,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 733,
          "cds_start": 1405,
          "cds_end": null,
          "cds_length": 2202,
          "cdna_start": 1637,
          "cdna_end": null,
          "cdna_length": 5832,
          "mane_select": "NM_021135.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000265678.9"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1138A>G",
          "hgvs_p": "p.Ile380Val",
          "transcript": "ENST00000481261.6",
          "protein_id": "ENSP00000422484.1",
          "transcript_support_level": 1,
          "aa_start": 380,
          "aa_end": null,
          "aa_length": 644,
          "cds_start": 1138,
          "cds_end": null,
          "cds_length": 1935,
          "cdna_start": 1397,
          "cdna_end": null,
          "cdna_length": 2297,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000481261.6"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1480A>G",
          "hgvs_p": "p.Ile494Val",
          "transcript": "NM_001318936.2",
          "protein_id": "NP_001305865.2",
          "transcript_support_level": null,
          "aa_start": 494,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 1480,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": 1835,
          "cdna_end": null,
          "cdna_length": 6030,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318936.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1480A>G",
          "hgvs_p": "p.Ile494Val",
          "transcript": "ENST00000510118.5",
          "protein_id": "ENSP00000422435.1",
          "transcript_support_level": 2,
          "aa_start": 494,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 1480,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": 1821,
          "cdna_end": null,
          "cdna_length": 5992,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000510118.5"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1480A>G",
          "hgvs_p": "p.Ile494Val",
          "transcript": "ENST00000967274.1",
          "protein_id": "ENSP00000637333.1",
          "transcript_support_level": null,
          "aa_start": 494,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 1480,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": 1760,
          "cdna_end": null,
          "cdna_length": 5925,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967274.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1429A>G",
          "hgvs_p": "p.Ile477Val",
          "transcript": "NM_001006932.3",
          "protein_id": "NP_001006933.3",
          "transcript_support_level": null,
          "aa_start": 477,
          "aa_end": null,
          "aa_length": 741,
          "cds_start": 1429,
          "cds_end": null,
          "cds_length": 2226,
          "cdna_start": 1784,
          "cdna_end": null,
          "cdna_length": 5979,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001006932.3"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1429A>G",
          "hgvs_p": "p.Ile477Val",
          "transcript": "ENST00000503859.5",
          "protein_id": "ENSP00000427015.1",
          "transcript_support_level": 2,
          "aa_start": 477,
          "aa_end": null,
          "aa_length": 741,
          "cds_start": 1429,
          "cds_end": null,
          "cds_length": 2226,
          "cdna_start": 1810,
          "cdna_end": null,
          "cdna_length": 4137,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000503859.5"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1372A>G",
          "hgvs_p": "p.Ile458Val",
          "transcript": "ENST00000907318.1",
          "protein_id": "ENSP00000577377.1",
          "transcript_support_level": null,
          "aa_start": 458,
          "aa_end": null,
          "aa_length": 722,
          "cds_start": 1372,
          "cds_end": null,
          "cds_length": 2169,
          "cdna_start": 1538,
          "cdna_end": null,
          "cdna_length": 3889,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000907318.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1357A>G",
          "hgvs_p": "p.Ile453Val",
          "transcript": "ENST00000714395.1",
          "protein_id": "ENSP00000519662.1",
          "transcript_support_level": null,
          "aa_start": 453,
          "aa_end": null,
          "aa_length": 717,
          "cds_start": 1357,
          "cds_end": null,
          "cds_length": 2154,
          "cdna_start": 1357,
          "cdna_end": null,
          "cdna_length": 5552,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000714395.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1285A>G",
          "hgvs_p": "p.Ile429Val",
          "transcript": "ENST00000907314.1",
          "protein_id": "ENSP00000577373.1",
          "transcript_support_level": null,
          "aa_start": 429,
          "aa_end": null,
          "aa_length": 693,
          "cds_start": 1285,
          "cds_end": null,
          "cds_length": 2082,
          "cdna_start": 1521,
          "cdna_end": null,
          "cdna_length": 3883,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000907314.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1405A>G",
          "hgvs_p": "p.Ile469Val",
          "transcript": "ENST00000935351.1",
          "protein_id": "ENSP00000605410.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 679,
          "cds_start": 1405,
          "cds_end": null,
          "cds_length": 2040,
          "cdna_start": 1637,
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          "cdna_length": 3826,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935351.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1405A>G",
          "hgvs_p": "p.Ile469Val",
          "transcript": "ENST00000907313.1",
          "protein_id": "ENSP00000577372.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 668,
          "cds_start": 1405,
          "cds_end": null,
          "cds_length": 2007,
          "cdna_start": 1668,
          "cdna_end": null,
          "cdna_length": 3824,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000907313.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1138A>G",
          "hgvs_p": "p.Ile380Val",
          "transcript": "NM_001318938.1",
          "protein_id": "NP_001305867.1",
          "transcript_support_level": null,
          "aa_start": 380,
          "aa_end": null,
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          "cds_start": 1138,
          "cds_end": null,
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          "cdna_start": 1397,
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          "cdna_length": 5592,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001318938.1"
        },
        {
          "aa_ref": "I",
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          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1138A>G",
          "hgvs_p": "p.Ile380Val",
          "transcript": "ENST00000405189.7",
          "protein_id": "ENSP00000386050.3",
          "transcript_support_level": 2,
          "aa_start": 380,
          "aa_end": null,
          "aa_length": 644,
          "cds_start": 1138,
          "cds_end": null,
          "cds_length": 1935,
          "cdna_start": 1471,
          "cdna_end": null,
          "cdna_length": 2282,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000405189.7"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1111A>G",
          "hgvs_p": "p.Ile371Val",
          "transcript": "NM_001318937.2",
          "protein_id": "NP_001305866.1",
          "transcript_support_level": null,
          "aa_start": 371,
          "aa_end": null,
          "aa_length": 635,
          "cds_start": 1111,
          "cds_end": null,
          "cds_length": 1908,
          "cdna_start": 1492,
          "cdna_end": null,
          "cdna_length": 5687,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318937.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1489A>G",
          "hgvs_p": "p.Ile497Val",
          "transcript": "XM_006715549.4",
          "protein_id": "XP_006715612.1",
          "transcript_support_level": null,
          "aa_start": 497,
          "aa_end": null,
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          "cds_start": 1489,
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          "cdna_start": 1721,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1399A>G",
          "hgvs_p": "p.Ile467Val",
          "transcript": "XM_047419233.1",
          "protein_id": "XP_047275189.1",
          "transcript_support_level": null,
          "aa_start": 467,
          "aa_end": null,
          "aa_length": 731,
          "cds_start": 1399,
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          "cdna_start": 1460,
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          "cdna_length": 5655,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047419233.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1357A>G",
          "hgvs_p": "p.Ile453Val",
          "transcript": "XM_047419234.1",
          "protein_id": "XP_047275190.1",
          "transcript_support_level": null,
          "aa_start": 453,
          "aa_end": null,
          "aa_length": 717,
          "cds_start": 1357,
          "cds_end": null,
          "cds_length": 2154,
          "cdna_start": 1686,
          "cdna_end": null,
          "cdna_length": 5881,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047419234.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS6KA2",
          "gene_hgnc_id": 10431,
          "hgvs_c": "c.1138A>G",
          "hgvs_p": "p.Ile380Val",
          "transcript": "XM_047419235.1",
          "protein_id": "XP_047275191.1",
          "transcript_support_level": null,
          "aa_start": 380,
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        {
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        {
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          "biotype": "protein_coding",
          "feature": "ENST00000907316.1"
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      ],
      "gene_symbol": "RPS6KA2",
      "gene_hgnc_id": 10431,
      "dbsnp": "rs75225867",
      "frequency_reference_population": 0.00017982756,
      "hom_count_reference_population": 0,
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      "gnomad_exomes_af": 0.000184889,
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      "gnomad_genomes_ac": 20,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.20108789205551147,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.086,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1033,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.47,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.594,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -6,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate,BS2",
      "acmg_by_gene": [
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001318936.2",
          "gene_symbol": "RPS6KA2",
          "hgnc_id": 10431,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1480A>G",
          "hgvs_p": "p.Ile494Val"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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