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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-2765656-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=2765656&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 2765656,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_020135.3",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WRNIP1",
          "gene_hgnc_id": 20876,
          "hgvs_c": "c.34C>G",
          "hgvs_p": "p.Leu12Val",
          "transcript": "NM_020135.3",
          "protein_id": "NP_064520.2",
          "transcript_support_level": null,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 665,
          "cds_start": 34,
          "cds_end": null,
          "cds_length": 1998,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000380773.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_020135.3"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WRNIP1",
          "gene_hgnc_id": 20876,
          "hgvs_c": "c.34C>G",
          "hgvs_p": "p.Leu12Val",
          "transcript": "ENST00000380773.9",
          "protein_id": "ENSP00000370150.4",
          "transcript_support_level": 1,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 665,
          "cds_start": 34,
          "cds_end": null,
          "cds_length": 1998,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_020135.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000380773.9"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WRNIP1",
          "gene_hgnc_id": 20876,
          "hgvs_c": "c.34C>G",
          "hgvs_p": "p.Leu12Val",
          "transcript": "ENST00000618555.4",
          "protein_id": "ENSP00000477551.1",
          "transcript_support_level": 1,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 665,
          "cds_start": 34,
          "cds_end": null,
          "cds_length": 1998,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000618555.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WRNIP1",
          "gene_hgnc_id": 20876,
          "hgvs_c": "c.34C>G",
          "hgvs_p": "p.Leu12Val",
          "transcript": "ENST00000380771.8",
          "protein_id": "ENSP00000370148.4",
          "transcript_support_level": 1,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 640,
          "cds_start": 34,
          "cds_end": null,
          "cds_length": 1923,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000380771.8"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WRNIP1",
          "gene_hgnc_id": 20876,
          "hgvs_c": "c.34C>G",
          "hgvs_p": "p.Leu12Val",
          "transcript": "NM_130395.3",
          "protein_id": "NP_569079.1",
          "transcript_support_level": null,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 640,
          "cds_start": 34,
          "cds_end": null,
          "cds_length": 1923,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_130395.3"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WRNIP1",
          "gene_hgnc_id": 20876,
          "hgvs_c": "c.34C>G",
          "hgvs_p": "p.Leu12Val",
          "transcript": "ENST00000922062.1",
          "protein_id": "ENSP00000592121.1",
          "transcript_support_level": null,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 613,
          "cds_start": 34,
          "cds_end": null,
          "cds_length": 1842,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922062.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WRNIP1",
          "gene_hgnc_id": 20876,
          "hgvs_c": "c.34C>G",
          "hgvs_p": "p.Leu12Val",
          "transcript": "ENST00000922063.1",
          "protein_id": "ENSP00000592122.1",
          "transcript_support_level": null,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 34,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922063.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WRNIP1",
          "gene_hgnc_id": 20876,
          "hgvs_c": "c.34C>G",
          "hgvs_p": "p.Leu12Val",
          "transcript": "XM_017011060.2",
          "protein_id": "XP_016866549.1",
          "transcript_support_level": null,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 438,
          "cds_start": 34,
          "cds_end": null,
          "cds_length": 1317,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017011060.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WRNIP1",
          "gene_hgnc_id": 20876,
          "hgvs_c": "c.34C>G",
          "hgvs_p": "p.Leu12Val",
          "transcript": "XM_005249232.4",
          "protein_id": "XP_005249289.1",
          "transcript_support_level": null,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 34,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005249232.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK4",
          "gene_hgnc_id": 27972,
          "hgvs_c": "c.-540G>C",
          "hgvs_p": null,
          "transcript": "ENST00000866488.1",
          "protein_id": "ENSP00000536547.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 388,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1167,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866488.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK4",
          "gene_hgnc_id": 27972,
          "hgvs_c": "c.-422G>C",
          "hgvs_p": null,
          "transcript": "ENST00000866489.1",
          "protein_id": "ENSP00000536548.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 388,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1167,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866489.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK4",
          "gene_hgnc_id": 27972,
          "hgvs_c": "c.-426G>C",
          "hgvs_p": null,
          "transcript": "ENST00000941557.1",
          "protein_id": "ENSP00000611616.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 388,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1167,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000941557.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK4",
          "gene_hgnc_id": 27972,
          "hgvs_c": "c.-183G>C",
          "hgvs_p": null,
          "transcript": "XM_005249078.5",
          "protein_id": "XP_005249135.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 469,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1410,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005249078.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "WRNIP1",
          "gene_hgnc_id": 20876,
          "hgvs_c": "c.-484C>G",
          "hgvs_p": null,
          "transcript": "XM_047419078.1",
          "protein_id": "XP_047275034.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 348,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1047,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047419078.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "MYLK4",
          "gene_hgnc_id": 27972,
          "hgvs_c": "c.56+4402G>C",
          "hgvs_p": null,
          "transcript": "NM_001347872.2",
          "protein_id": "NP_001334801.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 444,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1335,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001347872.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "MYLK4",
          "gene_hgnc_id": 27972,
          "hgvs_c": "c.56+4402G>C",
          "hgvs_p": null,
          "transcript": "ENST00000698899.1",
          "protein_id": "ENSP00000514016.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 444,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1335,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000698899.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MYLK4",
          "gene_hgnc_id": 27972,
          "hgvs_c": "n.-183G>C",
          "hgvs_p": null,
          "transcript": "XR_926185.4",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_926185.4"
        }
      ],
      "gene_symbol": "WRNIP1",
      "gene_hgnc_id": 20876,
      "dbsnp": "rs776806803",
      "frequency_reference_population": 7.167133e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 7.16713e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.3797934055328369,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.134,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1216,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.35,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.577,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_020135.3",
          "gene_symbol": "WRNIP1",
          "hgnc_id": 20876,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.34C>G",
          "hgvs_p": "p.Leu12Val"
        },
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001347872.2",
          "gene_symbol": "MYLK4",
          "hgnc_id": 27972,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.56+4402G>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}