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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-30740497-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=30740497&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 30740497,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "NM_005803.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.569G>A",
          "hgvs_p": "p.Arg190Gln",
          "transcript": "NM_005803.4",
          "protein_id": "NP_005794.1",
          "transcript_support_level": null,
          "aa_start": 190,
          "aa_end": null,
          "aa_length": 427,
          "cds_start": 569,
          "cds_end": null,
          "cds_length": 1284,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000376389.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005803.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.569G>A",
          "hgvs_p": "p.Arg190Gln",
          "transcript": "ENST00000376389.8",
          "protein_id": "ENSP00000365569.3",
          "transcript_support_level": 1,
          "aa_start": 190,
          "aa_end": null,
          "aa_length": 427,
          "cds_start": 569,
          "cds_end": null,
          "cds_length": 1284,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_005803.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000376389.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.569G>A",
          "hgvs_p": "p.Arg190Gln",
          "transcript": "ENST00000445853.5",
          "protein_id": "ENSP00000398834.1",
          "transcript_support_level": 1,
          "aa_start": 190,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 569,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000445853.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.674G>A",
          "hgvs_p": "p.Arg225Gln",
          "transcript": "ENST00000903950.1",
          "protein_id": "ENSP00000574009.1",
          "transcript_support_level": null,
          "aa_start": 225,
          "aa_end": null,
          "aa_length": 462,
          "cds_start": 674,
          "cds_end": null,
          "cds_length": 1389,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903950.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.608G>A",
          "hgvs_p": "p.Arg203Gln",
          "transcript": "ENST00000914089.1",
          "protein_id": "ENSP00000584148.1",
          "transcript_support_level": null,
          "aa_start": 203,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 608,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914089.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.608G>A",
          "hgvs_p": "p.Arg203Gln",
          "transcript": "ENST00000946582.1",
          "protein_id": "ENSP00000616641.1",
          "transcript_support_level": null,
          "aa_start": 203,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 608,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946582.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.569G>A",
          "hgvs_p": "p.Arg190Gln",
          "transcript": "ENST00000903944.1",
          "protein_id": "ENSP00000574003.1",
          "transcript_support_level": null,
          "aa_start": 190,
          "aa_end": null,
          "aa_length": 427,
          "cds_start": 569,
          "cds_end": null,
          "cds_length": 1284,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903944.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.569G>A",
          "hgvs_p": "p.Arg190Gln",
          "transcript": "ENST00000903945.1",
          "protein_id": "ENSP00000574004.1",
          "transcript_support_level": null,
          "aa_start": 190,
          "aa_end": null,
          "aa_length": 427,
          "cds_start": 569,
          "cds_end": null,
          "cds_length": 1284,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903945.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.569G>A",
          "hgvs_p": "p.Arg190Gln",
          "transcript": "ENST00000903948.1",
          "protein_id": "ENSP00000574007.1",
          "transcript_support_level": null,
          "aa_start": 190,
          "aa_end": null,
          "aa_length": 427,
          "cds_start": 569,
          "cds_end": null,
          "cds_length": 1284,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903948.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.569G>A",
          "hgvs_p": "p.Arg190Gln",
          "transcript": "ENST00000903949.1",
          "protein_id": "ENSP00000574008.1",
          "transcript_support_level": null,
          "aa_start": 190,
          "aa_end": null,
          "aa_length": 427,
          "cds_start": 569,
          "cds_end": null,
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          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903949.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.566G>A",
          "hgvs_p": "p.Arg189Gln",
          "transcript": "ENST00000903959.1",
          "protein_id": "ENSP00000574018.1",
          "transcript_support_level": null,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 426,
          "cds_start": 566,
          "cds_end": null,
          "cds_length": 1281,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.566G>A",
          "hgvs_p": "p.Arg189Gln",
          "transcript": "ENST00000914092.1",
          "protein_id": "ENSP00000584151.1",
          "transcript_support_level": null,
          "aa_start": 189,
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          "cds_start": 566,
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          "cds_length": 1281,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914092.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 7,
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.563G>A",
          "hgvs_p": "p.Arg188Gln",
          "transcript": "ENST00000903953.1",
          "protein_id": "ENSP00000574012.1",
          "transcript_support_level": null,
          "aa_start": 188,
          "aa_end": null,
          "aa_length": 425,
          "cds_start": 563,
          "cds_end": null,
          "cds_length": 1278,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903953.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
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          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.563G>A",
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          "transcript": "ENST00000946577.1",
          "protein_id": "ENSP00000616636.1",
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        },
        {
          "aa_ref": "R",
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          "canonical": false,
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          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 13,
          "intron_rank": null,
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          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.569G>A",
          "hgvs_p": "p.Arg190Gln",
          "transcript": "ENST00000914090.1",
          "protein_id": "ENSP00000584149.1",
          "transcript_support_level": null,
          "aa_start": 190,
          "aa_end": null,
          "aa_length": 424,
          "cds_start": 569,
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          "cds_length": 1275,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
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          "strand": false,
          "consequences": [
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            "splice_region_variant"
          ],
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          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
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          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.557G>A",
          "hgvs_p": "p.Arg186Gln",
          "transcript": "ENST00000946579.1",
          "protein_id": "ENSP00000616638.1",
          "transcript_support_level": null,
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          "aa_length": 423,
          "cds_start": 557,
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          "cds_length": 1272,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000946579.1"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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            "splice_region_variant"
          ],
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          "intron_rank": null,
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          "hgvs_c": "c.569G>A",
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          "transcript": "ENST00000903951.1",
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        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.545G>A",
          "hgvs_p": "p.Arg182Gln",
          "transcript": "ENST00000903956.1",
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          "cds_start": 545,
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        {
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          "strand": false,
          "consequences": [
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          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FLOT1",
          "gene_hgnc_id": 3757,
          "hgvs_c": "c.533G>A",
          "hgvs_p": "p.Arg178Gln",
          "transcript": "ENST00000903952.1",
          "protein_id": "ENSP00000574011.1",
          "transcript_support_level": null,
          "aa_start": 178,
          "aa_end": null,
          "aa_length": 415,
          "cds_start": 533,
          "cds_end": null,
          "cds_length": 1248,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903952.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
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        {
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        {
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          "gene_symbol": "FLOT1",
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          "hgvs_c": "c.*12G>A",
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          "transcript": "ENST00000454845.1",
          "protein_id": "ENSP00000391341.1",
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          "biotype": "protein_coding",
          "feature": "ENST00000454845.1"
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      ],
      "gene_symbol": "FLOT1",
      "gene_hgnc_id": 3757,
      "dbsnp": "rs754804649",
      "frequency_reference_population": 0.00006201966,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 100,
      "gnomad_exomes_af": 0.0000623232,
      "gnomad_genomes_af": 0.0000591086,
      "gnomad_exomes_ac": 91,
      "gnomad_genomes_ac": 9,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.18395259976387024,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.5720000267028809,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.201,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0811,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.14,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.844,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.939830810826487,
      "dbscsnv_ada_prediction": "Benign",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_005803.4",
          "gene_symbol": "FLOT1",
          "hgnc_id": 3757,
          "effects": [
            "missense_variant",
            "splice_region_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.569G>A",
          "hgvs_p": "p.Arg190Gln"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}