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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-31886812-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=31886812&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 31886812,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001363689.2",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "c.2204G>A",
          "hgvs_p": "p.Arg735His",
          "transcript": "NM_006709.5",
          "protein_id": "NP_006700.3",
          "transcript_support_level": null,
          "aa_start": 735,
          "aa_end": null,
          "aa_length": 1210,
          "cds_start": 2204,
          "cds_end": null,
          "cds_length": 3633,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000375537.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_006709.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "c.2204G>A",
          "hgvs_p": "p.Arg735His",
          "transcript": "ENST00000375537.9",
          "protein_id": "ENSP00000364687.4",
          "transcript_support_level": 1,
          "aa_start": 735,
          "aa_end": null,
          "aa_length": 1210,
          "cds_start": 2204,
          "cds_end": null,
          "cds_length": 3633,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_006709.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375537.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "c.2375G>A",
          "hgvs_p": "p.Arg792His",
          "transcript": "ENST00000395728.7",
          "protein_id": "ENSP00000379078.3",
          "transcript_support_level": 1,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 1267,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 3804,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395728.7"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "c.2375G>A",
          "hgvs_p": "p.Arg792His",
          "transcript": "NM_001363689.2",
          "protein_id": "NP_001350618.1",
          "transcript_support_level": null,
          "aa_start": 792,
          "aa_end": null,
          "aa_length": 1267,
          "cds_start": 2375,
          "cds_end": null,
          "cds_length": 3804,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001363689.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "c.2204G>A",
          "hgvs_p": "p.Arg735His",
          "transcript": "ENST00000962959.1",
          "protein_id": "ENSP00000633018.1",
          "transcript_support_level": null,
          "aa_start": 735,
          "aa_end": null,
          "aa_length": 1251,
          "cds_start": 2204,
          "cds_end": null,
          "cds_length": 3756,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000962959.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "c.2204G>A",
          "hgvs_p": "p.Arg735His",
          "transcript": "ENST00000962960.1",
          "protein_id": "ENSP00000633019.1",
          "transcript_support_level": null,
          "aa_start": 735,
          "aa_end": null,
          "aa_length": 1241,
          "cds_start": 2204,
          "cds_end": null,
          "cds_length": 3726,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000962960.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "c.2204G>A",
          "hgvs_p": "p.Arg735His",
          "transcript": "ENST00000892701.1",
          "protein_id": "ENSP00000562760.1",
          "transcript_support_level": null,
          "aa_start": 735,
          "aa_end": null,
          "aa_length": 1240,
          "cds_start": 2204,
          "cds_end": null,
          "cds_length": 3723,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892701.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "c.2273G>A",
          "hgvs_p": "p.Arg758His",
          "transcript": "NM_001289413.2",
          "protein_id": "NP_001276342.1",
          "transcript_support_level": null,
          "aa_start": 758,
          "aa_end": null,
          "aa_length": 1233,
          "cds_start": 2273,
          "cds_end": null,
          "cds_length": 3702,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001289413.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "c.2273G>A",
          "hgvs_p": "p.Arg758His",
          "transcript": "ENST00000375528.8",
          "protein_id": "ENSP00000364678.4",
          "transcript_support_level": 2,
          "aa_start": 758,
          "aa_end": null,
          "aa_length": 1233,
          "cds_start": 2273,
          "cds_end": null,
          "cds_length": 3702,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375528.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "c.2204G>A",
          "hgvs_p": "p.Arg735His",
          "transcript": "ENST00000892698.1",
          "protein_id": "ENSP00000562757.1",
          "transcript_support_level": null,
          "aa_start": 735,
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          "aa_length": 1227,
          "cds_start": 2204,
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          "cds_length": 3684,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        {
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
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          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "c.2231G>A",
          "hgvs_p": "p.Arg744His",
          "transcript": "ENST00000892705.1",
          "protein_id": "ENSP00000562764.1",
          "transcript_support_level": null,
          "aa_start": 744,
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          "aa_length": 1219,
          "cds_start": 2231,
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          "cds_length": 3660,
          "cdna_start": null,
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        {
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          "consequences": [
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          "intron_rank": null,
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          "gene_symbol": "EHMT2",
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          "hgvs_c": "c.2204G>A",
          "hgvs_p": "p.Arg735His",
          "transcript": "NM_001395160.1",
          "protein_id": "NP_001382089.1",
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          "cds_start": 2204,
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        {
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          ],
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          "gene_symbol": "EHMT2",
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          "transcript": "ENST00000892695.1",
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        {
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          "strand": false,
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          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
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          "gene_symbol": "EHMT2",
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          "hgvs_c": "c.2204G>A",
          "hgvs_p": "p.Arg735His",
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        {
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          "gene_symbol": "EHMT2",
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        },
        {
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          ],
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          "exon_count": 28,
          "intron_rank": null,
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          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "c.2135G>A",
          "hgvs_p": "p.Arg712His",
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          "aa_end": null,
          "aa_length": 1195,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3588,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892703.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": 16,
          "intron_rank_end": null,
          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "c.2094+8G>A",
          "hgvs_p": null,
          "transcript": "ENST00000920075.1",
          "protein_id": "ENSP00000590134.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1161,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3486,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920075.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "n.308G>A",
          "hgvs_p": null,
          "transcript": "ENST00000477678.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000477678.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EHMT2",
          "gene_hgnc_id": 14129,
          "hgvs_c": "n.2165G>A",
          "hgvs_p": null,
          "transcript": "ENST00000480912.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000480912.5"
        }
      ],
      "gene_symbol": "EHMT2",
      "gene_hgnc_id": 14129,
      "dbsnp": "rs765936779",
      "frequency_reference_population": 0.000010944826,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 16,
      "gnomad_exomes_af": 0.0000109448,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 16,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.3566591739654541,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.03999999910593033,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.296,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.0882,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.12,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.395,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.04,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001363689.2",
          "gene_symbol": "EHMT2",
          "hgnc_id": 14129,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2375G>A",
          "hgvs_p": "p.Arg792His"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}