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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-35122238-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=35122238&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 35122238,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001370687.1",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.457G>T",
          "hgvs_p": "p.Ala153Ser",
          "transcript": "NM_001370687.1",
          "protein_id": "NP_001357616.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 503,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 1512,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000311875.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001370687.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.457G>T",
          "hgvs_p": "p.Ala153Ser",
          "transcript": "ENST00000311875.11",
          "protein_id": "ENSP00000308708.6",
          "transcript_support_level": 1,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 503,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 1512,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001370687.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000311875.11"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.457G>T",
          "hgvs_p": "p.Ala153Ser",
          "transcript": "ENST00000512012.5",
          "protein_id": "ENSP00000425995.1",
          "transcript_support_level": 1,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 503,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 1512,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000512012.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.271G>T",
          "hgvs_p": "p.Ala91Ser",
          "transcript": "ENST00000244645.7",
          "protein_id": "ENSP00000244645.3",
          "transcript_support_level": 1,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 441,
          "cds_start": 271,
          "cds_end": null,
          "cds_length": 1326,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000244645.7"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.268G>T",
          "hgvs_p": "p.Ala90Ser",
          "transcript": "ENST00000611141.4",
          "protein_id": "ENSP00000478603.1",
          "transcript_support_level": 1,
          "aa_start": 90,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 268,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000611141.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "n.*425G>T",
          "hgvs_p": null,
          "transcript": "ENST00000455706.7",
          "protein_id": "ENSP00000399012.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000455706.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "n.*537G>T",
          "hgvs_p": null,
          "transcript": "ENST00000504758.6",
          "protein_id": "ENSP00000421916.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000504758.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "n.*522G>T",
          "hgvs_p": null,
          "transcript": "ENST00000505911.6",
          "protein_id": "ENSP00000423293.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000505911.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "n.*425G>T",
          "hgvs_p": null,
          "transcript": "ENST00000455706.7",
          "protein_id": "ENSP00000399012.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000455706.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "n.*537G>T",
          "hgvs_p": null,
          "transcript": "ENST00000504758.6",
          "protein_id": "ENSP00000421916.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000504758.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "n.*522G>T",
          "hgvs_p": null,
          "transcript": "ENST00000505911.6",
          "protein_id": "ENSP00000423293.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000505911.6"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.496G>T",
          "hgvs_p": "p.Ala166Ser",
          "transcript": "ENST00000673754.1",
          "protein_id": "ENSP00000501201.1",
          "transcript_support_level": null,
          "aa_start": 166,
          "aa_end": null,
          "aa_length": 516,
          "cds_start": 496,
          "cds_end": null,
          "cds_length": 1551,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000673754.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.442G>T",
          "hgvs_p": "p.Ala148Ser",
          "transcript": "NM_001261817.2",
          "protein_id": "NP_001248746.2",
          "transcript_support_level": null,
          "aa_start": 148,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 442,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001261817.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.442G>T",
          "hgvs_p": "p.Ala148Ser",
          "transcript": "ENST00000444780.7",
          "protein_id": "ENSP00000404479.3",
          "transcript_support_level": 2,
          "aa_start": 148,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 442,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000444780.7"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.358G>T",
          "hgvs_p": "p.Ala120Ser",
          "transcript": "NM_001261818.2",
          "protein_id": "NP_001248747.1",
          "transcript_support_level": null,
          "aa_start": 120,
          "aa_end": null,
          "aa_length": 470,
          "cds_start": 358,
          "cds_end": null,
          "cds_length": 1413,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001261818.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.358G>T",
          "hgvs_p": "p.Ala120Ser",
          "transcript": "ENST00000373974.8",
          "protein_id": "ENSP00000363085.4",
          "transcript_support_level": 2,
          "aa_start": 120,
          "aa_end": null,
          "aa_length": 470,
          "cds_start": 358,
          "cds_end": null,
          "cds_length": 1413,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000373974.8"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.343G>T",
          "hgvs_p": "p.Ala115Ser",
          "transcript": "NM_001261819.2",
          "protein_id": "NP_001248748.1",
          "transcript_support_level": null,
          "aa_start": 115,
          "aa_end": null,
          "aa_length": 465,
          "cds_start": 343,
          "cds_end": null,
          "cds_length": 1398,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001261819.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.343G>T",
          "hgvs_p": "p.Ala115Ser",
          "transcript": "ENST00000412155.6",
          "protein_id": "ENSP00000402816.2",
          "transcript_support_level": 2,
          "aa_start": 115,
          "aa_end": null,
          "aa_length": 465,
          "cds_start": 343,
          "cds_end": null,
          "cds_length": 1398,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000412155.6"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.271G>T",
          "hgvs_p": "p.Ala91Ser",
          "transcript": "NM_001366324.2",
          "protein_id": "NP_001353253.1",
          "transcript_support_level": null,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 441,
          "cds_start": 271,
          "cds_end": null,
          "cds_length": 1326,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001366324.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCP11",
          "gene_hgnc_id": 11658,
          "hgvs_c": "c.271G>T",
          "hgvs_p": "p.Ala91Ser",
          "transcript": "NM_018679.6",
          "protein_id": "NP_061149.1",
          "transcript_support_level": null,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 441,
          "cds_start": 271,
          "cds_end": null,
          "cds_length": 1326,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_018679.6"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
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        {
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000509988.6"
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      ],
      "gene_symbol": "TCP11",
      "gene_hgnc_id": 11658,
      "dbsnp": "rs375000730",
      "frequency_reference_population": 0.000121434714,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 196,
      "gnomad_exomes_af": 0.000125864,
      "gnomad_genomes_af": 0.0000788716,
      "gnomad_exomes_ac": 184,
      "gnomad_genomes_ac": 12,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.3105071187019348,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.117,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0839,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.59,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.562,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -1,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4",
      "acmg_by_gene": [
        {
          "score": -1,
          "benign_score": 1,
          "pathogenic_score": 0,
          "criteria": [
            "BP4"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001370687.1",
          "gene_symbol": "TCP11",
          "hgnc_id": 11658,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.457G>T",
          "hgvs_p": "p.Ala153Ser"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}