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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-36293081-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=36293081&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 36293081,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000457797.5",
      "consequences": [
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNPLA1",
          "gene_hgnc_id": 21246,
          "hgvs_c": "c.459C>G",
          "hgvs_p": "p.Phe153Leu",
          "transcript": "NM_001374623.1",
          "protein_id": "NP_001361552.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 572,
          "cds_start": 459,
          "cds_end": null,
          "cds_length": 1719,
          "cdna_start": 859,
          "cdna_end": null,
          "cdna_length": 4188,
          "mane_select": "ENST00000636260.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNPLA1",
          "gene_hgnc_id": 21246,
          "hgvs_c": "c.459C>G",
          "hgvs_p": "p.Phe153Leu",
          "transcript": "ENST00000636260.2",
          "protein_id": "ENSP00000490785.2",
          "transcript_support_level": 5,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 572,
          "cds_start": 459,
          "cds_end": null,
          "cds_length": 1719,
          "cdna_start": 859,
          "cdna_end": null,
          "cdna_length": 4188,
          "mane_select": "NM_001374623.1",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNPLA1",
          "gene_hgnc_id": 21246,
          "hgvs_c": "c.462C>G",
          "hgvs_p": "p.Phe154Leu",
          "transcript": "ENST00000457797.5",
          "protein_id": "ENSP00000391868.1",
          "transcript_support_level": 1,
          "aa_start": 154,
          "aa_end": null,
          "aa_length": 533,
          "cds_start": 462,
          "cds_end": null,
          "cds_length": 1602,
          "cdna_start": 462,
          "cdna_end": null,
          "cdna_length": 1602,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNPLA1",
          "gene_hgnc_id": 21246,
          "hgvs_c": "c.459C>G",
          "hgvs_p": "p.Phe153Leu",
          "transcript": "ENST00000394571.3",
          "protein_id": "ENSP00000378072.2",
          "transcript_support_level": 1,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 532,
          "cds_start": 459,
          "cds_end": null,
          "cds_length": 1599,
          "cdna_start": 459,
          "cdna_end": null,
          "cdna_length": 1599,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNPLA1",
          "gene_hgnc_id": 21246,
          "hgvs_c": "c.174C>G",
          "hgvs_p": "p.Phe58Leu",
          "transcript": "ENST00000312917.9",
          "protein_id": "ENSP00000321116.5",
          "transcript_support_level": 1,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 446,
          "cds_start": 174,
          "cds_end": null,
          "cds_length": 1341,
          "cdna_start": 313,
          "cdna_end": null,
          "cdna_length": 1518,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNPLA1",
          "gene_hgnc_id": 21246,
          "hgvs_c": "c.174C>G",
          "hgvs_p": "p.Phe58Leu",
          "transcript": "ENST00000388715.7",
          "protein_id": "ENSP00000373367.3",
          "transcript_support_level": 1,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 437,
          "cds_start": 174,
          "cds_end": null,
          "cds_length": 1314,
          "cdna_start": 313,
          "cdna_end": null,
          "cdna_length": 2332,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNPLA1",
          "gene_hgnc_id": 21246,
          "hgvs_c": "c.-73C>G",
          "hgvs_p": null,
          "transcript": "XM_017010779.2",
          "protein_id": "XP_016866268.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3532,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNPLA1",
          "gene_hgnc_id": 21246,
          "hgvs_c": "c.459C>G",
          "hgvs_p": "p.Phe153Leu",
          "transcript": "NM_001145717.1",
          "protein_id": "NP_001139189.2",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 532,
          "cds_start": 459,
          "cds_end": null,
          "cds_length": 1599,
          "cdna_start": 459,
          "cdna_end": null,
          "cdna_length": 2478,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNPLA1",
          "gene_hgnc_id": 21246,
          "hgvs_c": "c.174C>G",
          "hgvs_p": "p.Phe58Leu",
          "transcript": "NM_001145716.2",
          "protein_id": "NP_001139188.1",
          "transcript_support_level": null,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 446,
          "cds_start": 174,
          "cds_end": null,
          "cds_length": 1341,
          "cdna_start": 348,
          "cdna_end": null,
          "cdna_length": 2394,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNPLA1",
          "gene_hgnc_id": 21246,
          "hgvs_c": "c.174C>G",
          "hgvs_p": "p.Phe58Leu",
          "transcript": "NM_173676.2",
          "protein_id": "NP_775947.2",
          "transcript_support_level": null,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 437,
          "cds_start": 174,
          "cds_end": null,
          "cds_length": 1314,
          "cdna_start": 348,
          "cdna_end": null,
          "cdna_length": 2367,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNPLA1",
          "gene_hgnc_id": 21246,
          "hgvs_c": "c.459C>G",
          "hgvs_p": "p.Phe153Leu",
          "transcript": "XM_047418678.1",
          "protein_id": "XP_047274634.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 552,
          "cds_start": 459,
          "cds_end": null,
          "cds_length": 1659,
          "cdna_start": 859,
          "cdna_end": null,
          "cdna_length": 3216,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNPLA1",
          "gene_hgnc_id": 21246,
          "hgvs_c": "c.174C>G",
          "hgvs_p": "p.Phe58Leu",
          "transcript": "XM_011514520.3",
          "protein_id": "XP_011512822.1",
          "transcript_support_level": null,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 477,
          "cds_start": 174,
          "cds_end": null,
          "cds_length": 1434,
          "cdna_start": 348,
          "cdna_end": null,
          "cdna_length": 3677,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNPLA1",
          "gene_hgnc_id": 21246,
          "hgvs_c": "c.-73C>G",
          "hgvs_p": null,
          "transcript": "XM_017010779.2",
          "protein_id": "XP_016866268.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3532,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "PNPLA1",
      "gene_hgnc_id": 21246,
      "dbsnp": "rs2239795",
      "frequency_reference_population": 6.8413397e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84134e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8571985960006714,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.642,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.999,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.15,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": -1.087,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000457797.5",
          "gene_symbol": "PNPLA1",
          "hgnc_id": 21246,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.462C>G",
          "hgvs_p": "p.Phe154Leu"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}