← Back to variant description

GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-41649706-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=41649706&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 41649706,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_005586.4",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "NM_005586.4",
          "protein_id": "NP_005577.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 246,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 741,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000230321.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005586.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "ENST00000230321.11",
          "protein_id": "ENSP00000230321.6",
          "transcript_support_level": 1,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 246,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 741,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_005586.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000230321.11"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "NM_001300804.2",
          "protein_id": "NP_001287733.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 246,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 741,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001300804.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "NM_001300806.2",
          "protein_id": "NP_001287735.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 246,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 741,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001300806.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "ENST00000373051.6",
          "protein_id": "ENSP00000362142.2",
          "transcript_support_level": 5,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 246,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 741,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000373051.6"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "ENST00000909785.1",
          "protein_id": "ENSP00000579844.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 246,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 741,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909785.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "ENST00000928623.1",
          "protein_id": "ENSP00000598682.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 246,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 741,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928623.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "ENST00000928624.1",
          "protein_id": "ENSP00000598683.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 246,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 741,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928624.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "ENST00000928626.1",
          "protein_id": "ENSP00000598685.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 246,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 741,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928626.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "ENST00000928627.1",
          "protein_id": "ENSP00000598686.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 246,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 741,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928627.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "ENST00000419164.6",
          "protein_id": "ENSP00000393881.1",
          "transcript_support_level": 5,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 230,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 694,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000419164.6"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.275C>T",
          "hgvs_p": "p.Ala92Val",
          "transcript": "ENST00000928625.1",
          "protein_id": "ENSP00000598684.1",
          "transcript_support_level": null,
          "aa_start": 92,
          "aa_end": null,
          "aa_length": 222,
          "cds_start": 275,
          "cds_end": null,
          "cds_length": 669,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928625.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "ENST00000432027.5",
          "protein_id": "ENSP00000413226.1",
          "transcript_support_level": 3,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 212,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 640,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000432027.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.164C>T",
          "hgvs_p": "p.Ala55Val",
          "transcript": "NM_001300805.2",
          "protein_id": "NP_001287734.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 185,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 558,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001300805.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.164C>T",
          "hgvs_p": "p.Ala55Val",
          "transcript": "ENST00000373050.8",
          "protein_id": "ENSP00000362141.4",
          "transcript_support_level": 2,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 185,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 558,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000373050.8"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.164C>T",
          "hgvs_p": "p.Ala55Val",
          "transcript": "ENST00000909784.1",
          "protein_id": "ENSP00000579843.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 185,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 558,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909784.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.164C>T",
          "hgvs_p": "p.Ala55Val",
          "transcript": "ENST00000435476.1",
          "protein_id": "ENSP00000403587.1",
          "transcript_support_level": 2,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 141,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 427,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000435476.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "ENST00000446650.1",
          "protein_id": "ENSP00000411829.1",
          "transcript_support_level": 2,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 127,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 384,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000446650.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "XM_047418777.1",
          "protein_id": "XP_047274733.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 246,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 741,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047418777.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val",
          "transcript": "XM_047418778.1",
          "protein_id": "XP_047274734.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 246,
          "cds_start": 347,
          "cds_end": null,
          "cds_length": 741,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047418778.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.296C>T",
          "hgvs_p": "p.Ala99Val",
          "transcript": "XM_005249117.4",
          "protein_id": "XP_005249174.1",
          "transcript_support_level": null,
          "aa_start": 99,
          "aa_end": null,
          "aa_length": 229,
          "cds_start": 296,
          "cds_end": null,
          "cds_length": 690,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005249117.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.296C>T",
          "hgvs_p": "p.Ala99Val",
          "transcript": "XM_011514625.3",
          "protein_id": "XP_011512927.1",
          "transcript_support_level": null,
          "aa_start": 99,
          "aa_end": null,
          "aa_length": 229,
          "cds_start": 296,
          "cds_end": null,
          "cds_length": 690,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011514625.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.296C>T",
          "hgvs_p": "p.Ala99Val",
          "transcript": "XM_047418779.1",
          "protein_id": "XP_047274735.1",
          "transcript_support_level": null,
          "aa_start": 99,
          "aa_end": null,
          "aa_length": 229,
          "cds_start": 296,
          "cds_end": null,
          "cds_length": 690,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047418779.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.296C>T",
          "hgvs_p": "p.Ala99Val",
          "transcript": "XM_047418780.1",
          "protein_id": "XP_047274736.1",
          "transcript_support_level": null,
          "aa_start": 99,
          "aa_end": null,
          "aa_length": 229,
          "cds_start": 296,
          "cds_end": null,
          "cds_length": 690,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047418780.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.167C>T",
          "hgvs_p": "p.Ala56Val",
          "transcript": "XM_017010867.2",
          "protein_id": "XP_016866356.1",
          "transcript_support_level": null,
          "aa_start": 56,
          "aa_end": null,
          "aa_length": 186,
          "cds_start": 167,
          "cds_end": null,
          "cds_length": 561,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017010867.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.167C>T",
          "hgvs_p": "p.Ala56Val",
          "transcript": "XM_047418781.1",
          "protein_id": "XP_047274737.1",
          "transcript_support_level": null,
          "aa_start": 56,
          "aa_end": null,
          "aa_length": 186,
          "cds_start": 167,
          "cds_end": null,
          "cds_length": 561,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047418781.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.164C>T",
          "hgvs_p": "p.Ala55Val",
          "transcript": "XM_011514626.3",
          "protein_id": "XP_011512928.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 185,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 558,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011514626.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "n.143C>T",
          "hgvs_p": null,
          "transcript": "ENST00000471092.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000471092.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MDFI",
          "gene_hgnc_id": 6967,
          "hgvs_c": "c.*53C>T",
          "hgvs_p": null,
          "transcript": "ENST00000441667.5",
          "protein_id": "ENSP00000406600.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 97,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 294,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000441667.5"
        }
      ],
      "gene_symbol": "MDFI",
      "gene_hgnc_id": 6967,
      "dbsnp": "rs150152866",
      "frequency_reference_population": 0.000029742172,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 48,
      "gnomad_exomes_af": 0.0000246298,
      "gnomad_genomes_af": 0.0000788291,
      "gnomad_exomes_ac": 36,
      "gnomad_genomes_ac": 12,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.10102054476737976,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.036,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0903,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.39,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.302,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_005586.4",
          "gene_symbol": "MDFI",
          "hgnc_id": 6967,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.347C>T",
          "hgvs_p": "p.Ala116Val"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}