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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-52504629-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=52504629&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 52504629,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_012288.4",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.1001C>T",
          "hgvs_p": "p.Thr334Ile",
          "transcript": "NM_012288.4",
          "protein_id": "NP_036420.1",
          "transcript_support_level": null,
          "aa_start": 334,
          "aa_end": null,
          "aa_length": 370,
          "cds_start": 1001,
          "cds_end": null,
          "cds_length": 1113,
          "cdna_start": 1146,
          "cdna_end": null,
          "cdna_length": 7047,
          "mane_select": "ENST00000182527.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_012288.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.1001C>T",
          "hgvs_p": "p.Thr334Ile",
          "transcript": "ENST00000182527.4",
          "protein_id": "ENSP00000182527.3",
          "transcript_support_level": 1,
          "aa_start": 334,
          "aa_end": null,
          "aa_length": 370,
          "cds_start": 1001,
          "cds_end": null,
          "cds_length": 1113,
          "cdna_start": 1146,
          "cdna_end": null,
          "cdna_length": 7047,
          "mane_select": "NM_012288.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000182527.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.1100C>T",
          "hgvs_p": "p.Thr367Ile",
          "transcript": "ENST00000956692.1",
          "protein_id": "ENSP00000626751.1",
          "transcript_support_level": null,
          "aa_start": 367,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": 1100,
          "cds_end": null,
          "cds_length": 1212,
          "cdna_start": 1257,
          "cdna_end": null,
          "cdna_length": 1599,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956692.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.1004C>T",
          "hgvs_p": "p.Thr335Ile",
          "transcript": "ENST00000956690.1",
          "protein_id": "ENSP00000626749.1",
          "transcript_support_level": null,
          "aa_start": 335,
          "aa_end": null,
          "aa_length": 371,
          "cds_start": 1004,
          "cds_end": null,
          "cds_length": 1116,
          "cdna_start": 1117,
          "cdna_end": null,
          "cdna_length": 3907,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956690.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.998C>T",
          "hgvs_p": "p.Thr333Ile",
          "transcript": "ENST00000912004.1",
          "protein_id": "ENSP00000582063.1",
          "transcript_support_level": null,
          "aa_start": 333,
          "aa_end": null,
          "aa_length": 369,
          "cds_start": 998,
          "cds_end": null,
          "cds_length": 1110,
          "cdna_start": 1143,
          "cdna_end": null,
          "cdna_length": 3931,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912004.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.992C>T",
          "hgvs_p": "p.Thr331Ile",
          "transcript": "ENST00000863425.1",
          "protein_id": "ENSP00000533484.1",
          "transcript_support_level": null,
          "aa_start": 331,
          "aa_end": null,
          "aa_length": 367,
          "cds_start": 992,
          "cds_end": null,
          "cds_length": 1104,
          "cdna_start": 1154,
          "cdna_end": null,
          "cdna_length": 3944,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000863425.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.968C>T",
          "hgvs_p": "p.Thr323Ile",
          "transcript": "ENST00000863427.1",
          "protein_id": "ENSP00000533486.1",
          "transcript_support_level": null,
          "aa_start": 323,
          "aa_end": null,
          "aa_length": 359,
          "cds_start": 968,
          "cds_end": null,
          "cds_length": 1080,
          "cdna_start": 1080,
          "cdna_end": null,
          "cdna_length": 1414,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000863427.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.896C>T",
          "hgvs_p": "p.Thr299Ile",
          "transcript": "ENST00000863426.1",
          "protein_id": "ENSP00000533485.1",
          "transcript_support_level": null,
          "aa_start": 299,
          "aa_end": null,
          "aa_length": 335,
          "cds_start": 896,
          "cds_end": null,
          "cds_length": 1008,
          "cdna_start": 1041,
          "cdna_end": null,
          "cdna_length": 3804,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000863426.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.884C>T",
          "hgvs_p": "p.Thr295Ile",
          "transcript": "ENST00000956694.1",
          "protein_id": "ENSP00000626753.1",
          "transcript_support_level": null,
          "aa_start": 295,
          "aa_end": null,
          "aa_length": 331,
          "cds_start": 884,
          "cds_end": null,
          "cds_length": 996,
          "cdna_start": 1008,
          "cdna_end": null,
          "cdna_length": 1350,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956694.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.767C>T",
          "hgvs_p": "p.Thr256Ile",
          "transcript": "ENST00000956695.1",
          "protein_id": "ENSP00000626754.1",
          "transcript_support_level": null,
          "aa_start": 256,
          "aa_end": null,
          "aa_length": 292,
          "cds_start": 767,
          "cds_end": null,
          "cds_length": 879,
          "cdna_start": 789,
          "cdna_end": null,
          "cdna_length": 1129,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956695.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.752C>T",
          "hgvs_p": "p.Thr251Ile",
          "transcript": "ENST00000956693.1",
          "protein_id": "ENSP00000626752.1",
          "transcript_support_level": null,
          "aa_start": 251,
          "aa_end": null,
          "aa_length": 287,
          "cds_start": 752,
          "cds_end": null,
          "cds_length": 864,
          "cdna_start": 893,
          "cdna_end": null,
          "cdna_length": 1233,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956693.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.710C>T",
          "hgvs_p": "p.Thr237Ile",
          "transcript": "ENST00000956691.1",
          "protein_id": "ENSP00000626750.1",
          "transcript_support_level": null,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 710,
          "cds_end": null,
          "cds_length": 822,
          "cdna_start": 839,
          "cdna_end": null,
          "cdna_length": 3602,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956691.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.596C>T",
          "hgvs_p": "p.Thr199Ile",
          "transcript": "ENST00000912005.1",
          "protein_id": "ENSP00000582064.1",
          "transcript_support_level": null,
          "aa_start": 199,
          "aa_end": null,
          "aa_length": 235,
          "cds_start": 596,
          "cds_end": null,
          "cds_length": 708,
          "cdna_start": 709,
          "cdna_end": null,
          "cdna_length": 3499,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912005.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRAM2",
          "gene_hgnc_id": 16855,
          "hgvs_c": "c.890C>T",
          "hgvs_p": "p.Thr297Ile",
          "transcript": "XM_011515005.3",
          "protein_id": "XP_011513307.1",
          "transcript_support_level": null,
          "aa_start": 297,
          "aa_end": null,
          "aa_length": 333,
          "cds_start": 890,
          "cds_end": null,
          "cds_length": 1002,
          "cdna_start": 1009,
          "cdna_end": null,
          "cdna_length": 6910,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011515005.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 10,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.1851+14279G>A",
          "hgvs_p": null,
          "transcript": "ENST00000637353.1",
          "protein_id": "ENSP00000490441.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 641,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1926,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3829,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000637353.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.1516-11266G>A",
          "hgvs_p": null,
          "transcript": "ENST00000636343.1",
          "protein_id": "ENSP00000490193.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 517,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1554,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2965,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000636343.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 10,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "n.*1552+14279G>A",
          "hgvs_p": null,
          "transcript": "ENST00000637602.1",
          "protein_id": "ENSP00000490074.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2020,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000637602.1"
        }
      ],
      "gene_symbol": "TRAM2",
      "gene_hgnc_id": 16855,
      "dbsnp": "rs199535862",
      "frequency_reference_population": 0.00028993553,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 468,
      "gnomad_exomes_af": 0.000301005,
      "gnomad_genomes_af": 0.000183749,
      "gnomad_exomes_ac": 440,
      "gnomad_genomes_ac": 28,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.03176814317703247,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.045,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.064,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.54,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.1,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -8,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BS2",
      "acmg_by_gene": [
        {
          "score": -8,
          "benign_score": 8,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_012288.4",
          "gene_symbol": "TRAM2",
          "hgnc_id": 16855,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1001C>T",
          "hgvs_p": "p.Thr334Ile"
        },
        {
          "score": -8,
          "benign_score": 8,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000637602.1",
          "gene_symbol": "EFHC1",
          "hgnc_id": 16406,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "n.*1552+14279G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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