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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-7404480-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=7404480&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 7404480,
      "ref": "A",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_031480.3",
      "consequences": [
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RIOK1",
          "gene_hgnc_id": 18656,
          "hgvs_c": "c.917A>C",
          "hgvs_p": "p.Gln306Pro",
          "transcript": "NM_031480.3",
          "protein_id": "NP_113668.2",
          "transcript_support_level": null,
          "aa_start": 306,
          "aa_end": null,
          "aa_length": 568,
          "cds_start": 917,
          "cds_end": null,
          "cds_length": 1707,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000379834.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_031480.3"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RIOK1",
          "gene_hgnc_id": 18656,
          "hgvs_c": "c.917A>C",
          "hgvs_p": "p.Gln306Pro",
          "transcript": "ENST00000379834.7",
          "protein_id": "ENSP00000369162.2",
          "transcript_support_level": 1,
          "aa_start": 306,
          "aa_end": null,
          "aa_length": 568,
          "cds_start": 917,
          "cds_end": null,
          "cds_length": 1707,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_031480.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000379834.7"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RIOK1",
          "gene_hgnc_id": 18656,
          "hgvs_c": "c.1010A>C",
          "hgvs_p": "p.Gln337Pro",
          "transcript": "ENST00000967571.1",
          "protein_id": "ENSP00000637630.1",
          "transcript_support_level": null,
          "aa_start": 337,
          "aa_end": null,
          "aa_length": 599,
          "cds_start": 1010,
          "cds_end": null,
          "cds_length": 1800,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967571.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RIOK1",
          "gene_hgnc_id": 18656,
          "hgvs_c": "c.1007A>C",
          "hgvs_p": "p.Gln336Pro",
          "transcript": "ENST00000911539.1",
          "protein_id": "ENSP00000581598.1",
          "transcript_support_level": null,
          "aa_start": 336,
          "aa_end": null,
          "aa_length": 598,
          "cds_start": 1007,
          "cds_end": null,
          "cds_length": 1797,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000911539.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RIOK1",
          "gene_hgnc_id": 18656,
          "hgvs_c": "c.932A>C",
          "hgvs_p": "p.Gln311Pro",
          "transcript": "ENST00000875537.1",
          "protein_id": "ENSP00000545596.1",
          "transcript_support_level": null,
          "aa_start": 311,
          "aa_end": null,
          "aa_length": 573,
          "cds_start": 932,
          "cds_end": null,
          "cds_length": 1722,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000875537.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RIOK1",
          "gene_hgnc_id": 18656,
          "hgvs_c": "c.917A>C",
          "hgvs_p": "p.Gln306Pro",
          "transcript": "ENST00000875536.1",
          "protein_id": "ENSP00000545595.1",
          "transcript_support_level": null,
          "aa_start": 306,
          "aa_end": null,
          "aa_length": 568,
          "cds_start": 917,
          "cds_end": null,
          "cds_length": 1707,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000875536.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RIOK1",
          "gene_hgnc_id": 18656,
          "hgvs_c": "c.917A>C",
          "hgvs_p": "p.Gln306Pro",
          "transcript": "ENST00000967569.1",
          "protein_id": "ENSP00000637628.1",
          "transcript_support_level": null,
          "aa_start": 306,
          "aa_end": null,
          "aa_length": 550,
          "cds_start": 917,
          "cds_end": null,
          "cds_length": 1653,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967569.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RIOK1",
          "gene_hgnc_id": 18656,
          "hgvs_c": "c.824A>C",
          "hgvs_p": "p.Gln275Pro",
          "transcript": "ENST00000967570.1",
          "protein_id": "ENSP00000637629.1",
          "transcript_support_level": null,
          "aa_start": 275,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 824,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967570.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RIOK1",
          "gene_hgnc_id": 18656,
          "hgvs_c": "c.764A>C",
          "hgvs_p": "p.Gln255Pro",
          "transcript": "ENST00000911540.1",
          "protein_id": "ENSP00000581599.1",
          "transcript_support_level": null,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 517,
          "cds_start": 764,
          "cds_end": null,
          "cds_length": 1554,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000911540.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RIOK1",
          "gene_hgnc_id": 18656,
          "hgvs_c": "c.605A>C",
          "hgvs_p": "p.Gln202Pro",
          "transcript": "NM_001348194.2",
          "protein_id": "NP_001335123.1",
          "transcript_support_level": null,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 605,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001348194.2"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RIOK1",
          "gene_hgnc_id": 18656,
          "hgvs_c": "c.953A>C",
          "hgvs_p": "p.Gln318Pro",
          "transcript": "XM_011514933.4",
          "protein_id": "XP_011513235.2",
          "transcript_support_level": null,
          "aa_start": 318,
          "aa_end": null,
          "aa_length": 580,
          "cds_start": 953,
          "cds_end": null,
          "cds_length": 1743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011514933.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RIOK1",
          "gene_hgnc_id": 18656,
          "hgvs_c": "n.602A>C",
          "hgvs_p": null,
          "transcript": "ENST00000264874.7",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000264874.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RIOK1",
          "gene_hgnc_id": 18656,
          "hgvs_c": "n.157A>C",
          "hgvs_p": null,
          "transcript": "ENST00000484626.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000484626.1"
        }
      ],
      "gene_symbol": "RIOK1",
      "gene_hgnc_id": 18656,
      "dbsnp": "rs1761691891",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8841085433959961,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.379,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.75,
      "alphamissense_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 7.018,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_031480.3",
          "gene_symbol": "RIOK1",
          "hgnc_id": 18656,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.917A>C",
          "hgvs_p": "p.Gln306Pro"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}