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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-83216581-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=83216581&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 83216581,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_002395.6",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.1465G>T",
          "hgvs_p": "p.Val489Leu",
          "transcript": "NM_002395.6",
          "protein_id": "NP_002386.1",
          "transcript_support_level": null,
          "aa_start": 489,
          "aa_end": null,
          "aa_length": 572,
          "cds_start": 1465,
          "cds_end": null,
          "cds_length": 1719,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000369705.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002395.6"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.1465G>T",
          "hgvs_p": "p.Val489Leu",
          "transcript": "ENST00000369705.4",
          "protein_id": "ENSP00000358719.3",
          "transcript_support_level": 1,
          "aa_start": 489,
          "aa_end": null,
          "aa_length": 572,
          "cds_start": 1465,
          "cds_end": null,
          "cds_length": 1719,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002395.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000369705.4"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.1579G>T",
          "hgvs_p": "p.Val527Leu",
          "transcript": "ENST00000956348.1",
          "protein_id": "ENSP00000626407.1",
          "transcript_support_level": null,
          "aa_start": 527,
          "aa_end": null,
          "aa_length": 610,
          "cds_start": 1579,
          "cds_end": null,
          "cds_length": 1833,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956348.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.1519G>T",
          "hgvs_p": "p.Val507Leu",
          "transcript": "ENST00000956344.1",
          "protein_id": "ENSP00000626403.1",
          "transcript_support_level": null,
          "aa_start": 507,
          "aa_end": null,
          "aa_length": 590,
          "cds_start": 1519,
          "cds_end": null,
          "cds_length": 1773,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956344.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.1462G>T",
          "hgvs_p": "p.Val488Leu",
          "transcript": "ENST00000956342.1",
          "protein_id": "ENSP00000626401.1",
          "transcript_support_level": null,
          "aa_start": 488,
          "aa_end": null,
          "aa_length": 571,
          "cds_start": 1462,
          "cds_end": null,
          "cds_length": 1716,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956342.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.1462G>T",
          "hgvs_p": "p.Val488Leu",
          "transcript": "ENST00000956343.1",
          "protein_id": "ENSP00000626402.1",
          "transcript_support_level": null,
          "aa_start": 488,
          "aa_end": null,
          "aa_length": 571,
          "cds_start": 1462,
          "cds_end": null,
          "cds_length": 1716,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956343.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.1399G>T",
          "hgvs_p": "p.Val467Leu",
          "transcript": "ENST00000901911.1",
          "protein_id": "ENSP00000571970.1",
          "transcript_support_level": null,
          "aa_start": 467,
          "aa_end": null,
          "aa_length": 550,
          "cds_start": 1399,
          "cds_end": null,
          "cds_length": 1653,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901911.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.1393G>T",
          "hgvs_p": "p.Val465Leu",
          "transcript": "ENST00000956347.1",
          "protein_id": "ENSP00000626406.1",
          "transcript_support_level": null,
          "aa_start": 465,
          "aa_end": null,
          "aa_length": 548,
          "cds_start": 1393,
          "cds_end": null,
          "cds_length": 1647,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956347.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.1315G>T",
          "hgvs_p": "p.Val439Leu",
          "transcript": "ENST00000901910.1",
          "protein_id": "ENSP00000571969.1",
          "transcript_support_level": null,
          "aa_start": 439,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 1315,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901910.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.1303G>T",
          "hgvs_p": "p.Val435Leu",
          "transcript": "ENST00000956346.1",
          "protein_id": "ENSP00000626405.1",
          "transcript_support_level": null,
          "aa_start": 435,
          "aa_end": null,
          "aa_length": 518,
          "cds_start": 1303,
          "cds_end": null,
          "cds_length": 1557,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956346.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.1291G>T",
          "hgvs_p": "p.Val431Leu",
          "transcript": "ENST00000901909.1",
          "protein_id": "ENSP00000571968.1",
          "transcript_support_level": null,
          "aa_start": 431,
          "aa_end": null,
          "aa_length": 514,
          "cds_start": 1291,
          "cds_end": null,
          "cds_length": 1545,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901909.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.1225G>T",
          "hgvs_p": "p.Val409Leu",
          "transcript": "ENST00000901913.1",
          "protein_id": "ENSP00000571972.1",
          "transcript_support_level": null,
          "aa_start": 409,
          "aa_end": null,
          "aa_length": 492,
          "cds_start": 1225,
          "cds_end": null,
          "cds_length": 1479,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901913.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.943G>T",
          "hgvs_p": "p.Val315Leu",
          "transcript": "ENST00000901912.1",
          "protein_id": "ENSP00000571971.1",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 398,
          "cds_start": 943,
          "cds_end": null,
          "cds_length": 1197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901912.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": 12,
          "intron_rank_end": null,
          "gene_symbol": "ME1",
          "gene_hgnc_id": 6983,
          "hgvs_c": "c.1450-4487G>T",
          "hgvs_p": null,
          "transcript": "ENST00000956345.1",
          "protein_id": "ENSP00000626404.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 539,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1620,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956345.1"
        }
      ],
      "gene_symbol": "ME1",
      "gene_hgnc_id": 6983,
      "dbsnp": "rs367907680",
      "frequency_reference_population": 0.000019893248,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 32,
      "gnomad_exomes_af": 0.0000205997,
      "gnomad_genomes_af": 0.0000131358,
      "gnomad_exomes_ac": 30,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.38822001218795776,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.141,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.8042,
      "alphamissense_prediction": "Pathogenic",
      "bayesdelnoaf_score": -0.14,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.474,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_002395.6",
          "gene_symbol": "ME1",
          "hgnc_id": 6983,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1465G>T",
          "hgvs_p": "p.Val489Leu"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}