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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 7-100803468-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=7&pos=100803468&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "7",
      "pos": 100803468,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_004444.5",
      "consequences": [
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2957A>C",
          "hgvs_p": "p.Gln986Pro",
          "transcript": "NM_004444.5",
          "protein_id": "NP_004435.3",
          "transcript_support_level": null,
          "aa_start": 986,
          "aa_end": null,
          "aa_length": 987,
          "cds_start": 2957,
          "cds_end": null,
          "cds_length": 2964,
          "cdna_start": 3450,
          "cdna_end": null,
          "cdna_length": 4353,
          "mane_select": "ENST00000358173.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004444.5"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2957A>C",
          "hgvs_p": "p.Gln986Pro",
          "transcript": "ENST00000358173.8",
          "protein_id": "ENSP00000350896.3",
          "transcript_support_level": 1,
          "aa_start": 986,
          "aa_end": null,
          "aa_length": 987,
          "cds_start": 2957,
          "cds_end": null,
          "cds_length": 2964,
          "cdna_start": 3450,
          "cdna_end": null,
          "cdna_length": 4353,
          "mane_select": "NM_004444.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000358173.8"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2801A>C",
          "hgvs_p": "p.Gln934Pro",
          "transcript": "ENST00000360620.7",
          "protein_id": "ENSP00000353833.3",
          "transcript_support_level": 1,
          "aa_start": 934,
          "aa_end": null,
          "aa_length": 935,
          "cds_start": 2801,
          "cds_end": null,
          "cds_length": 2808,
          "cdna_start": 2886,
          "cdna_end": null,
          "cdna_length": 3789,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000360620.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "n.4158A>C",
          "hgvs_p": null,
          "transcript": "ENST00000487222.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5058,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000487222.5"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.3131A>C",
          "hgvs_p": "p.Gln1044Pro",
          "transcript": "ENST00000922076.1",
          "protein_id": "ENSP00000592135.1",
          "transcript_support_level": null,
          "aa_start": 1044,
          "aa_end": null,
          "aa_length": 1045,
          "cds_start": 3131,
          "cds_end": null,
          "cds_length": 3138,
          "cdna_start": 3594,
          "cdna_end": null,
          "cdna_length": 4481,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922076.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.3122A>C",
          "hgvs_p": "p.Gln1041Pro",
          "transcript": "ENST00000922072.1",
          "protein_id": "ENSP00000592131.1",
          "transcript_support_level": null,
          "aa_start": 1041,
          "aa_end": null,
          "aa_length": 1042,
          "cds_start": 3122,
          "cds_end": null,
          "cds_length": 3129,
          "cdna_start": 3647,
          "cdna_end": null,
          "cdna_length": 4550,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922072.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.3011A>C",
          "hgvs_p": "p.Gln1004Pro",
          "transcript": "ENST00000856891.1",
          "protein_id": "ENSP00000526950.1",
          "transcript_support_level": null,
          "aa_start": 1004,
          "aa_end": null,
          "aa_length": 1005,
          "cds_start": 3011,
          "cds_end": null,
          "cds_length": 3018,
          "cdna_start": 3477,
          "cdna_end": null,
          "cdna_length": 4380,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856891.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2996A>C",
          "hgvs_p": "p.Gln999Pro",
          "transcript": "ENST00000856892.1",
          "protein_id": "ENSP00000526951.1",
          "transcript_support_level": null,
          "aa_start": 999,
          "aa_end": null,
          "aa_length": 1000,
          "cds_start": 2996,
          "cds_end": null,
          "cds_length": 3003,
          "cdna_start": 3437,
          "cdna_end": null,
          "cdna_length": 4339,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856892.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2969A>C",
          "hgvs_p": "p.Gln990Pro",
          "transcript": "ENST00000856893.1",
          "protein_id": "ENSP00000526952.1",
          "transcript_support_level": null,
          "aa_start": 990,
          "aa_end": null,
          "aa_length": 991,
          "cds_start": 2969,
          "cds_end": null,
          "cds_length": 2976,
          "cdna_start": 3308,
          "cdna_end": null,
          "cdna_length": 4213,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856893.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2945A>C",
          "hgvs_p": "p.Gln982Pro",
          "transcript": "ENST00000971336.1",
          "protein_id": "ENSP00000641395.1",
          "transcript_support_level": null,
          "aa_start": 982,
          "aa_end": null,
          "aa_length": 983,
          "cds_start": 2945,
          "cds_end": null,
          "cds_length": 2952,
          "cdna_start": 3398,
          "cdna_end": null,
          "cdna_length": 4285,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000971336.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2831A>C",
          "hgvs_p": "p.Gln944Pro",
          "transcript": "ENST00000922073.1",
          "protein_id": "ENSP00000592132.1",
          "transcript_support_level": null,
          "aa_start": 944,
          "aa_end": null,
          "aa_length": 945,
          "cds_start": 2831,
          "cds_end": null,
          "cds_length": 2838,
          "cdna_start": 3324,
          "cdna_end": null,
          "cdna_length": 4226,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922073.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2789A>C",
          "hgvs_p": "p.Gln930Pro",
          "transcript": "ENST00000856888.1",
          "protein_id": "ENSP00000526947.1",
          "transcript_support_level": null,
          "aa_start": 930,
          "aa_end": null,
          "aa_length": 931,
          "cds_start": 2789,
          "cds_end": null,
          "cds_length": 2796,
          "cdna_start": 3282,
          "cdna_end": null,
          "cdna_length": 4185,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856888.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2768A>C",
          "hgvs_p": "p.Gln923Pro",
          "transcript": "ENST00000922074.1",
          "protein_id": "ENSP00000592133.1",
          "transcript_support_level": null,
          "aa_start": 923,
          "aa_end": null,
          "aa_length": 924,
          "cds_start": 2768,
          "cds_end": null,
          "cds_length": 2775,
          "cdna_start": 3243,
          "cdna_end": null,
          "cdna_length": 4137,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922074.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2756A>C",
          "hgvs_p": "p.Gln919Pro",
          "transcript": "ENST00000971335.1",
          "protein_id": "ENSP00000641394.1",
          "transcript_support_level": null,
          "aa_start": 919,
          "aa_end": null,
          "aa_length": 920,
          "cds_start": 2756,
          "cds_end": null,
          "cds_length": 2763,
          "cdna_start": 3270,
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          "cdna_length": 4171,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000971335.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2666A>C",
          "hgvs_p": "p.Gln889Pro",
          "transcript": "ENST00000922075.1",
          "protein_id": "ENSP00000592134.1",
          "transcript_support_level": null,
          "aa_start": 889,
          "aa_end": null,
          "aa_length": 890,
          "cds_start": 2666,
          "cds_end": null,
          "cds_length": 2673,
          "cdna_start": 3146,
          "cdna_end": null,
          "cdna_length": 4033,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922075.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2624A>C",
          "hgvs_p": "p.Gln875Pro",
          "transcript": "ENST00000856889.1",
          "protein_id": "ENSP00000526948.1",
          "transcript_support_level": null,
          "aa_start": 875,
          "aa_end": null,
          "aa_length": 876,
          "cds_start": 2624,
          "cds_end": null,
          "cds_length": 2631,
          "cdna_start": 3111,
          "cdna_end": null,
          "cdna_length": 4014,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856889.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2468A>C",
          "hgvs_p": "p.Gln823Pro",
          "transcript": "ENST00000856890.1",
          "protein_id": "ENSP00000526949.1",
          "transcript_support_level": null,
          "aa_start": 823,
          "aa_end": null,
          "aa_length": 824,
          "cds_start": 2468,
          "cds_end": null,
          "cds_length": 2475,
          "cdna_start": 2960,
          "cdna_end": null,
          "cdna_length": 3852,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856890.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.3011A>C",
          "hgvs_p": "p.Gln1004Pro",
          "transcript": "XM_017011816.2",
          "protein_id": "XP_016867305.1",
          "transcript_support_level": null,
          "aa_start": 1004,
          "aa_end": null,
          "aa_length": 1005,
          "cds_start": 3011,
          "cds_end": null,
          "cds_length": 3018,
          "cdna_start": 3504,
          "cdna_end": null,
          "cdna_length": 4407,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017011816.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.*1422A>C",
          "hgvs_p": null,
          "transcript": "ENST00000616502.4",
          "protein_id": "ENSP00000482702.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 306,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 921,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3737,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000616502.4"
        }
      ],
      "gene_symbol": "EPHB4",
      "gene_hgnc_id": 3395,
      "dbsnp": "rs1442631621",
      "frequency_reference_population": 7.037902e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 7.0379e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.15311592817306519,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.213,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0528,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.3,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.186,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_004444.5",
          "gene_symbol": "EPHB4",
          "hgnc_id": 3395,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.2957A>C",
          "hgvs_p": "p.Gln986Pro"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}
For research and educational, non-commercial use only. Not for clinical or diagnostic use. GeneBe does not provide medical advice. Data use for AI modeling is prohibited: if used, the cost is $0.001 per byte of downloaded uncompressed data.