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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 7-100806419-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=7&pos=100806419&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "7",
      "pos": 100806419,
      "ref": "C",
      "alt": "T",
      "effect": "splice_donor_variant,intron_variant",
      "transcript": "NM_004444.5",
      "consequences": [
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2484+1G>A",
          "hgvs_p": null,
          "transcript": "NM_004444.5",
          "protein_id": "NP_004435.3",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 987,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2964,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000358173.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004444.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2484+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000358173.8",
          "protein_id": "ENSP00000350896.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 987,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2964,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_004444.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000358173.8"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2484+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000360620.7",
          "protein_id": "ENSP00000353833.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 935,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2808,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000360620.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 13,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "n.3685+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000487222.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000487222.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2484+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000922076.1",
          "protein_id": "ENSP00000592135.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1045,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3138,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922076.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2649+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000922072.1",
          "protein_id": "ENSP00000592131.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1042,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3129,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922072.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2538+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000856891.1",
          "protein_id": "ENSP00000526950.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1005,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3018,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856891.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2523+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000856892.1",
          "protein_id": "ENSP00000526951.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1000,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3003,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856892.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2496+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000856893.1",
          "protein_id": "ENSP00000526952.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 991,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2976,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856893.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2472+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000971336.1",
          "protein_id": "ENSP00000641395.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 983,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2952,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000971336.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2358+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000922073.1",
          "protein_id": "ENSP00000592132.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 945,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2838,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922073.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": 12,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2316+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000856888.1",
          "protein_id": "ENSP00000526947.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 931,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2796,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856888.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2295+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000922074.1",
          "protein_id": "ENSP00000592133.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 924,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2775,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922074.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2283+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000971335.1",
          "protein_id": "ENSP00000641394.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 920,
          "cds_start": null,
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          "cds_length": 2763,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000971335.1"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": 12,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2193+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000922075.1",
          "protein_id": "ENSP00000592134.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 890,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2673,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922075.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 13,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2151+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000856889.1",
          "protein_id": "ENSP00000526948.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 876,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2631,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856889.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": 12,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.1995+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000856890.1",
          "protein_id": "ENSP00000526949.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 824,
          "cds_start": null,
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          "cds_length": 2475,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000856890.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": 11,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.*949+1G>A",
          "hgvs_p": null,
          "transcript": "ENST00000616502.4",
          "protein_id": "ENSP00000482702.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 306,
          "cds_start": null,
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          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000616502.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "c.2538+1G>A",
          "hgvs_p": null,
          "transcript": "XM_017011816.2",
          "protein_id": "XP_016867305.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1005,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 3018,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017011816.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "n.964G>A",
          "hgvs_p": null,
          "transcript": "ENST00000478459.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000478459.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHB4",
          "gene_hgnc_id": 3395,
          "hgvs_c": "n.366G>A",
          "hgvs_p": null,
          "transcript": "ENST00000492403.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000492403.1"
        }
      ],
      "gene_symbol": "EPHB4",
      "gene_hgnc_id": 3395,
      "dbsnp": "rs927772349",
      "frequency_reference_population": 0.0000013694465,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 0.00000136945,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.25,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0.9279999732971191,
      "splice_prediction_selected": "Pathogenic",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.25,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.905,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.96,
      "spliceai_max_prediction": "Pathogenic",
      "dbscsnv_ada_score": 0.999966203349492,
      "dbscsnv_ada_prediction": "Pathogenic",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 12,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1_Moderate,PM2,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 12,
          "benign_score": 0,
          "pathogenic_score": 12,
          "criteria": [
            "PVS1_Moderate",
            "PM2",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "NM_004444.5",
          "gene_symbol": "EPHB4",
          "hgnc_id": 3395,
          "effects": [
            "splice_donor_variant",
            "intron_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.2484+1G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Capillary malformation-arteriovenous malformation 2,not provided",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:2 LP:2",
      "phenotype_combined": "Capillary malformation-arteriovenous malformation 2|not provided",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}