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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 7-117627664-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=7&pos=117627664&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "7",
      "pos": 117627664,
      "ref": "G",
      "alt": "A",
      "effect": "stop_gained",
      "transcript": "ENST00000003084.11",
      "consequences": [
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "c.3611G>A",
          "hgvs_p": "p.Trp1204*",
          "transcript": "NM_000492.4",
          "protein_id": "NP_000483.3",
          "transcript_support_level": null,
          "aa_start": 1204,
          "aa_end": null,
          "aa_length": 1480,
          "cds_start": 3611,
          "cds_end": null,
          "cds_length": 4443,
          "cdna_start": 3681,
          "cdna_end": null,
          "cdna_length": 6070,
          "mane_select": "ENST00000003084.11",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "c.3611G>A",
          "hgvs_p": "p.Trp1204*",
          "transcript": "ENST00000003084.11",
          "protein_id": "ENSP00000003084.6",
          "transcript_support_level": 1,
          "aa_start": 1204,
          "aa_end": null,
          "aa_length": 1480,
          "cds_start": 3611,
          "cds_end": null,
          "cds_length": 4443,
          "cdna_start": 3681,
          "cdna_end": null,
          "cdna_length": 6070,
          "mane_select": "NM_000492.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "c.3605G>A",
          "hgvs_p": "p.Trp1202*",
          "transcript": "ENST00000699602.1",
          "protein_id": "ENSP00000514471.1",
          "transcript_support_level": null,
          "aa_start": 1202,
          "aa_end": null,
          "aa_length": 1478,
          "cds_start": 3605,
          "cds_end": null,
          "cds_length": 4437,
          "cdna_start": 3712,
          "cdna_end": null,
          "cdna_length": 6024,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "c.3521G>A",
          "hgvs_p": "p.Trp1174*",
          "transcript": "ENST00000426809.5",
          "protein_id": "ENSP00000389119.1",
          "transcript_support_level": 5,
          "aa_start": 1174,
          "aa_end": null,
          "aa_length": 1437,
          "cds_start": 3521,
          "cds_end": null,
          "cds_length": 4316,
          "cdna_start": 3521,
          "cdna_end": null,
          "cdna_length": 4316,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "c.3428G>A",
          "hgvs_p": "p.Trp1143*",
          "transcript": "ENST00000649781.2",
          "protein_id": "ENSP00000497203.1",
          "transcript_support_level": null,
          "aa_start": 1143,
          "aa_end": null,
          "aa_length": 1419,
          "cds_start": 3428,
          "cds_end": null,
          "cds_length": 4260,
          "cdna_start": 3512,
          "cdna_end": null,
          "cdna_length": 5824,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "c.3185G>A",
          "hgvs_p": "p.Trp1062*",
          "transcript": "ENST00000699605.1",
          "protein_id": "ENSP00000514473.1",
          "transcript_support_level": null,
          "aa_start": 1062,
          "aa_end": null,
          "aa_length": 1338,
          "cds_start": 3185,
          "cds_end": null,
          "cds_length": 4017,
          "cdna_start": 3607,
          "cdna_end": null,
          "cdna_length": 5919,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "c.3428G>A",
          "hgvs_p": "p.Trp1143*",
          "transcript": "ENST00000649406.1",
          "protein_id": "ENSP00000497965.1",
          "transcript_support_level": null,
          "aa_start": 1143,
          "aa_end": null,
          "aa_length": 1187,
          "cds_start": 3428,
          "cds_end": null,
          "cds_length": 3564,
          "cdna_start": 3512,
          "cdna_end": null,
          "cdna_length": 3758,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "c.2393G>A",
          "hgvs_p": "p.Trp798*",
          "transcript": "ENST00000648260.1",
          "protein_id": "ENSP00000497957.1",
          "transcript_support_level": null,
          "aa_start": 798,
          "aa_end": null,
          "aa_length": 842,
          "cds_start": 2393,
          "cds_end": null,
          "cds_length": 2529,
          "cdna_start": 2477,
          "cdna_end": null,
          "cdna_length": 2723,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "c.191G>A",
          "hgvs_p": "p.Trp64*",
          "transcript": "ENST00000689011.1",
          "protein_id": "ENSP00000510587.1",
          "transcript_support_level": null,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 279,
          "cds_start": 191,
          "cds_end": null,
          "cds_length": 840,
          "cdna_start": 193,
          "cdna_end": null,
          "cdna_length": 2842,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "c.434G>A",
          "hgvs_p": "p.Trp145*",
          "transcript": "ENST00000468795.1",
          "protein_id": "ENSP00000419254.1",
          "transcript_support_level": 5,
          "aa_start": 145,
          "aa_end": null,
          "aa_length": 189,
          "cds_start": 434,
          "cds_end": null,
          "cds_length": 570,
          "cdna_start": 436,
          "cdna_end": null,
          "cdna_length": 682,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "n.*3325G>A",
          "hgvs_p": null,
          "transcript": "ENST00000647978.2",
          "protein_id": "ENSP00000497658.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5898,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "n.3611G>A",
          "hgvs_p": null,
          "transcript": "ENST00000685018.2",
          "protein_id": "ENSP00000510194.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6223,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "n.*264G>A",
          "hgvs_p": null,
          "transcript": "ENST00000687278.2",
          "protein_id": "ENSP00000509593.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4888,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "n.3611G>A",
          "hgvs_p": null,
          "transcript": "ENST00000699598.1",
          "protein_id": "ENSP00000514467.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4931,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "n.3611G>A",
          "hgvs_p": null,
          "transcript": "ENST00000699599.1",
          "protein_id": "ENSP00000514468.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5054,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "n.*272G>A",
          "hgvs_p": null,
          "transcript": "ENST00000699600.1",
          "protein_id": "ENSP00000514469.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4793,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "n.*1986G>A",
          "hgvs_p": null,
          "transcript": "ENST00000699601.1",
          "protein_id": "ENSP00000514470.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6100,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "n.*3435G>A",
          "hgvs_p": null,
          "transcript": "ENST00000699604.1",
          "protein_id": "ENSP00000514472.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6005,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "n.*3325G>A",
          "hgvs_p": null,
          "transcript": "ENST00000647978.2",
          "protein_id": "ENSP00000497658.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5898,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "n.*264G>A",
          "hgvs_p": null,
          "transcript": "ENST00000687278.2",
          "protein_id": "ENSP00000509593.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4888,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CFTR",
          "gene_hgnc_id": 1884,
          "hgvs_c": "n.*272G>A",
          "hgvs_p": null,
          "transcript": "ENST00000699600.1",
          "protein_id": "ENSP00000514469.1",
          "transcript_support_level": null,
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      ],
      "gene_symbol": "CFTR",
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      "dbsnp": "rs121908764",
      "frequency_reference_population": 6.8439147e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84391e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.1850000023841858,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "REVEL",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.185,
      "revel_prediction": "Benign",
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.66,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 8.89,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
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      "acmg_score": 18,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5_Very_Strong",
      "acmg_by_gene": [
        {
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          "benign_score": 0,
          "pathogenic_score": 18,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000003084.11",
          "gene_symbol": "CFTR",
          "hgnc_id": 1884,
          "effects": [
            "stop_gained"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.3611G>A",
          "hgvs_p": "p.Trp1204*"
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        {
          "score": 10,
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          "pathogenic_score": 11,
          "criteria": [
            "PM2",
            "PP3",
            "PP5_Very_Strong",
            "BP4"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000456270.1",
          "gene_symbol": "ENSG00000083622",
          "hgnc_id": 40145,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.66-11324C>T",
          "hgvs_p": null
        },
        {
          "score": 10,
          "benign_score": 1,
          "pathogenic_score": 11,
          "criteria": [
            "PM2",
            "PP3",
            "PP5_Very_Strong",
            "BP4"
          ],
          "verdict": "Pathogenic",
          "transcript": "NR_199597.1",
          "gene_symbol": "CFTR-AS2",
          "hgnc_id": 40145,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.66-11324C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "CFTR-related disorder,Cystic fibrosis",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "reviewed by expert panel",
      "clinvar_submissions_summary": "P:4",
      "phenotype_combined": "Cystic fibrosis|CFTR-related disorder",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}