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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 7-128313185-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=7&pos=128313185&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "7",
      "pos": 128313185,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_018077.3",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBM28",
          "gene_hgnc_id": 21863,
          "hgvs_c": "c.2135C>T",
          "hgvs_p": "p.Ser712Leu",
          "transcript": "NM_018077.3",
          "protein_id": "NP_060547.2",
          "transcript_support_level": null,
          "aa_start": 712,
          "aa_end": null,
          "aa_length": 759,
          "cds_start": 2135,
          "cds_end": null,
          "cds_length": 2280,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000223073.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_018077.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBM28",
          "gene_hgnc_id": 21863,
          "hgvs_c": "c.2135C>T",
          "hgvs_p": "p.Ser712Leu",
          "transcript": "ENST00000223073.6",
          "protein_id": "ENSP00000223073.1",
          "transcript_support_level": 1,
          "aa_start": 712,
          "aa_end": null,
          "aa_length": 759,
          "cds_start": 2135,
          "cds_end": null,
          "cds_length": 2280,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_018077.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000223073.6"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBM28",
          "gene_hgnc_id": 21863,
          "hgvs_c": "c.2201C>T",
          "hgvs_p": "p.Ser734Leu",
          "transcript": "ENST00000899022.1",
          "protein_id": "ENSP00000569080.1",
          "transcript_support_level": null,
          "aa_start": 734,
          "aa_end": null,
          "aa_length": 781,
          "cds_start": 2201,
          "cds_end": null,
          "cds_length": 2346,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899022.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBM28",
          "gene_hgnc_id": 21863,
          "hgvs_c": "c.2126C>T",
          "hgvs_p": "p.Ser709Leu",
          "transcript": "ENST00000968249.1",
          "protein_id": "ENSP00000638308.1",
          "transcript_support_level": null,
          "aa_start": 709,
          "aa_end": null,
          "aa_length": 756,
          "cds_start": 2126,
          "cds_end": null,
          "cds_length": 2271,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968249.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBM28",
          "gene_hgnc_id": 21863,
          "hgvs_c": "c.2120C>T",
          "hgvs_p": "p.Ser707Leu",
          "transcript": "ENST00000937193.1",
          "protein_id": "ENSP00000607252.1",
          "transcript_support_level": null,
          "aa_start": 707,
          "aa_end": null,
          "aa_length": 754,
          "cds_start": 2120,
          "cds_end": null,
          "cds_length": 2265,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000937193.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBM28",
          "gene_hgnc_id": 21863,
          "hgvs_c": "c.2060C>T",
          "hgvs_p": "p.Ser687Leu",
          "transcript": "ENST00000968247.1",
          "protein_id": "ENSP00000638306.1",
          "transcript_support_level": null,
          "aa_start": 687,
          "aa_end": null,
          "aa_length": 734,
          "cds_start": 2060,
          "cds_end": null,
          "cds_length": 2205,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968247.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBM28",
          "gene_hgnc_id": 21863,
          "hgvs_c": "c.1925C>T",
          "hgvs_p": "p.Ser642Leu",
          "transcript": "ENST00000899025.1",
          "protein_id": "ENSP00000569083.1",
          "transcript_support_level": null,
          "aa_start": 642,
          "aa_end": null,
          "aa_length": 689,
          "cds_start": 1925,
          "cds_end": null,
          "cds_length": 2070,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899025.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBM28",
          "gene_hgnc_id": 21863,
          "hgvs_c": "c.1910C>T",
          "hgvs_p": "p.Ser637Leu",
          "transcript": "ENST00000899023.1",
          "protein_id": "ENSP00000569082.1",
          "transcript_support_level": null,
          "aa_start": 637,
          "aa_end": null,
          "aa_length": 684,
          "cds_start": 1910,
          "cds_end": null,
          "cds_length": 2055,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899023.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBM28",
          "gene_hgnc_id": 21863,
          "hgvs_c": "c.1712C>T",
          "hgvs_p": "p.Ser571Leu",
          "transcript": "NM_001166135.2",
          "protein_id": "NP_001159607.1",
          "transcript_support_level": null,
          "aa_start": 571,
          "aa_end": null,
          "aa_length": 618,
          "cds_start": 1712,
          "cds_end": null,
          "cds_length": 1857,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001166135.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBM28",
          "gene_hgnc_id": 21863,
          "hgvs_c": "c.1712C>T",
          "hgvs_p": "p.Ser571Leu",
          "transcript": "ENST00000415472.6",
          "protein_id": "ENSP00000390517.2",
          "transcript_support_level": 2,
          "aa_start": 571,
          "aa_end": null,
          "aa_length": 618,
          "cds_start": 1712,
          "cds_end": null,
          "cds_length": 1857,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000415472.6"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBM28",
          "gene_hgnc_id": 21863,
          "hgvs_c": "c.1640C>T",
          "hgvs_p": "p.Ser547Leu",
          "transcript": "ENST00000968248.1",
          "protein_id": "ENSP00000638307.1",
          "transcript_support_level": null,
          "aa_start": 547,
          "aa_end": null,
          "aa_length": 594,
          "cds_start": 1640,
          "cds_end": null,
          "cds_length": 1785,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968248.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBM28",
          "gene_hgnc_id": 21863,
          "hgvs_c": "n.507C>T",
          "hgvs_p": null,
          "transcript": "ENST00000481788.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000481788.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBM28",
          "gene_hgnc_id": 21863,
          "hgvs_c": "n.298C>T",
          "hgvs_p": null,
          "transcript": "ENST00000495327.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000495327.1"
        }
      ],
      "gene_symbol": "RBM28",
      "gene_hgnc_id": 21863,
      "dbsnp": "rs73230638",
      "frequency_reference_population": 0.0037542877,
      "hom_count_reference_population": 13,
      "allele_count_reference_population": 6059,
      "gnomad_exomes_af": 0.00386284,
      "gnomad_genomes_af": 0.00271229,
      "gnomad_exomes_ac": 5646,
      "gnomad_genomes_ac": 413,
      "gnomad_exomes_homalt": 11,
      "gnomad_genomes_homalt": 2,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.005905568599700928,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.072,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0729,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.55,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.246,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -16,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BS2",
      "acmg_by_gene": [
        {
          "score": -16,
          "benign_score": 16,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_018077.3",
          "gene_symbol": "RBM28",
          "hgnc_id": 21863,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2135C>T",
          "hgvs_p": "p.Ser712Leu"
        }
      ],
      "clinvar_disease": "RBM28-related disorder,not provided",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:3",
      "phenotype_combined": "not provided|RBM28-related disorder",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}