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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 7-135362941-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=7&pos=135362941&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "7",
      "pos": 135362941,
      "ref": "G",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001190850.2",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.2086C>A",
          "hgvs_p": "p.Pro696Thr",
          "transcript": "NM_001190850.2",
          "protein_id": "NP_001177779.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 713,
          "cds_start": 2086,
          "cds_end": null,
          "cds_length": 2142,
          "cdna_start": 2392,
          "cdna_end": null,
          "cdna_length": 3538,
          "mane_select": "ENST00000541284.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001190850.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.2086C>A",
          "hgvs_p": "p.Pro696Thr",
          "transcript": "ENST00000541284.6",
          "protein_id": "ENSP00000445508.1",
          "transcript_support_level": 5,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 713,
          "cds_start": 2086,
          "cds_end": null,
          "cds_length": 2142,
          "cdna_start": 2392,
          "cdna_end": null,
          "cdna_length": 3538,
          "mane_select": "NM_001190850.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000541284.6"
        },
        {
          "aa_ref": "P",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.1873C>A",
          "hgvs_p": "p.Pro625Thr",
          "transcript": "ENST00000423368.6",
          "protein_id": "ENSP00000406777.2",
          "transcript_support_level": 1,
          "aa_start": 625,
          "aa_end": null,
          "aa_length": 642,
          "cds_start": 1873,
          "cds_end": null,
          "cds_length": 1929,
          "cdna_start": 2154,
          "cdna_end": null,
          "cdna_length": 3297,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000423368.6"
        },
        {
          "aa_ref": "P",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.1864C>A",
          "hgvs_p": "p.Pro622Thr",
          "transcript": "ENST00000361528.8",
          "protein_id": "ENSP00000354673.4",
          "transcript_support_level": 1,
          "aa_start": 622,
          "aa_end": null,
          "aa_length": 639,
          "cds_start": 1864,
          "cds_end": null,
          "cds_length": 1920,
          "cdna_start": 2159,
          "cdna_end": null,
          "cdna_length": 2215,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000361528.8"
        },
        {
          "aa_ref": "P",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.2086C>A",
          "hgvs_p": "p.Pro696Thr",
          "transcript": "NM_001393370.1",
          "protein_id": "NP_001380299.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 713,
          "cds_start": 2086,
          "cds_end": null,
          "cds_length": 2142,
          "cdna_start": 2479,
          "cdna_end": null,
          "cdna_length": 3625,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001393370.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.2077C>A",
          "hgvs_p": "p.Pro693Thr",
          "transcript": "NM_001190849.2",
          "protein_id": "NP_001177778.1",
          "transcript_support_level": null,
          "aa_start": 693,
          "aa_end": null,
          "aa_length": 710,
          "cds_start": 2077,
          "cds_end": null,
          "cds_length": 2133,
          "cdna_start": 2383,
          "cdna_end": null,
          "cdna_length": 3529,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001190849.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.2077C>A",
          "hgvs_p": "p.Pro693Thr",
          "transcript": "ENST00000451834.5",
          "protein_id": "ENSP00000388491.1",
          "transcript_support_level": 2,
          "aa_start": 693,
          "aa_end": null,
          "aa_length": 710,
          "cds_start": 2077,
          "cds_end": null,
          "cds_length": 2133,
          "cdna_start": 2361,
          "cdna_end": null,
          "cdna_length": 3507,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000451834.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.2011C>A",
          "hgvs_p": "p.Pro671Thr",
          "transcript": "NM_001393371.1",
          "protein_id": "NP_001380300.1",
          "transcript_support_level": null,
          "aa_start": 671,
          "aa_end": null,
          "aa_length": 688,
          "cds_start": 2011,
          "cds_end": null,
          "cds_length": 2067,
          "cdna_start": 2317,
          "cdna_end": null,
          "cdna_length": 3463,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001393371.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.1999C>A",
          "hgvs_p": "p.Pro667Thr",
          "transcript": "ENST00000870230.1",
          "protein_id": "ENSP00000540289.1",
          "transcript_support_level": null,
          "aa_start": 667,
          "aa_end": null,
          "aa_length": 684,
          "cds_start": 1999,
          "cds_end": null,
          "cds_length": 2055,
          "cdna_start": 2313,
          "cdna_end": null,
          "cdna_length": 3412,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000870230.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
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          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.1975C>A",
          "hgvs_p": "p.Pro659Thr",
          "transcript": "NM_001393372.1",
          "protein_id": "NP_001380301.1",
          "transcript_support_level": null,
          "aa_start": 659,
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          "aa_length": 676,
          "cds_start": 1975,
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          "cdna_start": 2281,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "P",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
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          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.1888C>A",
          "hgvs_p": "p.Pro630Thr",
          "transcript": "NM_001393373.1",
          "protein_id": "NP_001380302.1",
          "transcript_support_level": null,
          "aa_start": 630,
          "aa_end": null,
          "aa_length": 647,
          "cds_start": 1888,
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          "cds_length": 1944,
          "cdna_start": 2194,
          "cdna_end": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "P",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          "exon_count": 11,
          "intron_rank": null,
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          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.1873C>A",
          "hgvs_p": "p.Pro625Thr",
          "transcript": "NM_001190847.2",
          "protein_id": "NP_001177776.1",
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          "cds_start": 1873,
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          "cdna_start": 2179,
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          "mane_select": null,
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        },
        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "exon_count": 12,
          "intron_rank": null,
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          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.1864C>A",
          "hgvs_p": "p.Pro622Thr",
          "transcript": "NM_001393374.1",
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          "cdna_start": 2257,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "P",
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          "canonical": false,
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          "consequences": [
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          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
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          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.1864C>A",
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          "transcript": "NM_013316.4",
          "protein_id": "NP_037448.2",
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        {
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          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.1864C>A",
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          "transcript": "ENST00000912981.1",
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          "feature": "ENST00000912981.1"
        },
        {
          "aa_ref": "P",
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          "consequences": [
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          "intron_rank": null,
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          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.1762C>A",
          "hgvs_p": "p.Pro588Thr",
          "transcript": "NM_001393375.1",
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          "cdna_start": 2068,
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        },
        {
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          ],
          "exon_rank": 12,
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          "intron_rank": null,
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          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "c.2077C>A",
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          "transcript": "XM_047420412.1",
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        {
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          "gene_symbol": "CNOT4",
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          "hgvs_c": "c.1873C>A",
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        },
        {
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          "consequences": [
            "3_prime_UTR_variant"
          ],
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          "intron_rank": null,
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          "gene_symbol": "CNOT4",
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          "hgvs_c": "c.*291C>A",
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          "transcript": "ENST00000707062.1",
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000707062.1"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "n.*58C>A",
          "hgvs_p": null,
          "transcript": "ENST00000473470.1",
          "protein_id": null,
          "transcript_support_level": 2,
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          "cdna_end": null,
          "cdna_length": 707,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000473470.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNOT4",
          "gene_hgnc_id": 7880,
          "hgvs_c": "n.*1498C>A",
          "hgvs_p": null,
          "transcript": "ENST00000707063.1",
          "protein_id": "ENSP00000516714.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2453,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000707063.1"
        }
      ],
      "gene_symbol": "CNOT4",
      "gene_hgnc_id": 7880,
      "dbsnp": "rs201606362",
      "frequency_reference_population": 0.000013021447,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 21,
      "gnomad_exomes_af": 0.0000136873,
      "gnomad_genomes_af": 0.00000659988,
      "gnomad_exomes_ac": 20,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.35379558801651,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.14,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1483,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.24,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.802,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4,BS2",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
            "BP4",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001190850.2",
          "gene_symbol": "CNOT4",
          "hgnc_id": 7880,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.2086C>A",
          "hgvs_p": "p.Pro696Thr"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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