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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 7-141708977-CAAAG-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=7&pos=141708977&ref=CAAAG&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "message": null,
  "variants": [
    {
      "acmg_by_gene": [
        {
          "benign_score": 0,
          "criteria": [
            "PVS1",
            "PS3",
            "PP5_Moderate"
          ],
          "effects": [
            "frameshift_variant"
          ],
          "gene_symbol": "WEE2",
          "hgnc_id": 19684,
          "hgvs_c": "c.224_227delAAAG",
          "hgvs_p": "p.Glu75fs",
          "inheritance_mode": "AR",
          "pathogenic_score": 14,
          "score": 14,
          "transcript": "NM_001105558.1",
          "verdict": "Pathogenic"
        },
        {
          "benign_score": 0,
          "criteria": [
            "PS3",
            "PP5_Moderate"
          ],
          "effects": [
            "intron_variant"
          ],
          "gene_symbol": "WEE2-AS1",
          "hgnc_id": 48669,
          "hgvs_c": "n.599-3224_599-3221delCTTT",
          "hgvs_p": null,
          "inheritance_mode": "",
          "pathogenic_score": 6,
          "score": 6,
          "transcript": "ENST00000462383.6",
          "verdict": "Likely_pathogenic"
        }
      ],
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PS3,PP5_Moderate",
      "acmg_score": 14,
      "allele_count_reference_population": 94,
      "alphamissense_prediction": null,
      "alphamissense_score": null,
      "alt": "C",
      "apogee2_prediction": null,
      "apogee2_score": null,
      "bayesdelnoaf_prediction": null,
      "bayesdelnoaf_score": null,
      "chr": "7",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_disease": "Oocyte maturation defect 5,WEE2-related disorder",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LP:1",
      "computational_prediction_selected": null,
      "computational_score_selected": null,
      "computational_source_selected": null,
      "consequences": [
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 567,
          "aa_ref": "ES",
          "aa_start": 75,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3061,
          "cdna_start": 630,
          "cds_end": null,
          "cds_length": 1704,
          "cds_start": 224,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_count": 12,
          "exon_rank": 1,
          "exon_rank_end": null,
          "feature": "NM_001105558.1",
          "gene_hgnc_id": 19684,
          "gene_symbol": "WEE2",
          "hgvs_c": "c.224_227delAAAG",
          "hgvs_p": "p.Glu75fs",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "ENST00000397541.6",
          "protein_coding": true,
          "protein_id": "NP_001099028.1",
          "strand": true,
          "transcript": "NM_001105558.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 567,
          "aa_ref": "ES",
          "aa_start": 75,
          "biotype": "protein_coding",
          "canonical": true,
          "cdna_end": null,
          "cdna_length": 3061,
          "cdna_start": 630,
          "cds_end": null,
          "cds_length": 1704,
          "cds_start": 224,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_count": 12,
          "exon_rank": 1,
          "exon_rank_end": null,
          "feature": "ENST00000397541.6",
          "gene_hgnc_id": 19684,
          "gene_symbol": "WEE2",
          "hgvs_c": "c.224_227delAAAG",
          "hgvs_p": "p.Glu75fs",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "NM_001105558.1",
          "protein_coding": true,
          "protein_id": "ENSP00000380675.2",
          "strand": true,
          "transcript": "ENST00000397541.6",
          "transcript_support_level": 1
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1993,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 5,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000462383.6",
          "gene_hgnc_id": 48669,
          "gene_symbol": "WEE2-AS1",
          "hgvs_c": "n.599-3224_599-3221delCTTT",
          "hgvs_p": null,
          "intron_rank": 4,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000462383.6",
          "transcript_support_level": 1
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1258,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 3,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000465110.5",
          "gene_hgnc_id": 48669,
          "gene_symbol": "WEE2-AS1",
          "hgvs_c": "n.133-3224_133-3221delCTTT",
          "hgvs_p": null,
          "intron_rank": 2,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000465110.5",
          "transcript_support_level": 1
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2259,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 7,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000478332.5",
          "gene_hgnc_id": 48669,
          "gene_symbol": "WEE2-AS1",
          "hgvs_c": "n.1134-3224_1134-3221delCTTT",
          "hgvs_p": null,
          "intron_rank": 6,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000478332.5",
          "transcript_support_level": 1
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1412,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 4,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000495800.5",
          "gene_hgnc_id": 48669,
          "gene_symbol": "WEE2-AS1",
          "hgvs_c": "n.287-3224_287-3221delCTTT",
          "hgvs_p": null,
          "intron_rank": 3,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000495800.5",
          "transcript_support_level": 1
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 567,
          "aa_ref": "ES",
          "aa_start": 75,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2956,
          "cdna_start": 529,
          "cds_end": null,
          "cds_length": 1704,
          "cds_start": 224,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_count": 13,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "ENST00000956320.1",
          "gene_hgnc_id": 19684,
          "gene_symbol": "WEE2",
          "hgvs_c": "c.224_227delAAAG",
          "hgvs_p": "p.Glu75fs",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000626379.1",
          "strand": true,
          "transcript": "ENST00000956320.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 565,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 5,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000459753.5",
          "gene_hgnc_id": 48669,
          "gene_symbol": "WEE2-AS1",
          "hgvs_c": "n.504-3224_504-3221delCTTT",
          "hgvs_p": null,
          "intron_rank": 4,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000459753.5",
          "transcript_support_level": 4
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 580,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 6,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000471512.5",
          "gene_hgnc_id": 48669,
          "gene_symbol": "WEE2-AS1",
          "hgvs_c": "n.525+2777_525+2780delCTTT",
          "hgvs_p": null,
          "intron_rank": 5,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000471512.5",
          "transcript_support_level": 4
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2262,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 7,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000488785.5",
          "gene_hgnc_id": 48669,
          "gene_symbol": "WEE2-AS1",
          "hgvs_c": "n.843-3224_843-3221delCTTT",
          "hgvs_p": null,
          "intron_rank": 6,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000488785.5",
          "transcript_support_level": 2
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 338,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 3,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000660874.1",
          "gene_hgnc_id": 48669,
          "gene_symbol": "WEE2-AS1",
          "hgvs_c": "n.220-1056_220-1053delCTTT",
          "hgvs_p": null,
          "intron_rank": 2,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000660874.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1822,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 5,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000664486.1",
          "gene_hgnc_id": 48669,
          "gene_symbol": "WEE2-AS1",
          "hgvs_c": "n.497-3224_497-3221delCTTT",
          "hgvs_p": null,
          "intron_rank": 4,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000664486.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2372,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 6,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000665340.1",
          "gene_hgnc_id": 48669,
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          "hgvs_c": "n.618-3224_618-3221delCTTT",
          "hgvs_p": null,
          "intron_rank": 5,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000665340.1",
          "transcript_support_level": null
        },
        {
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          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2261,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 7,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000666407.1",
          "gene_hgnc_id": 48669,
          "gene_symbol": "WEE2-AS1",
          "hgvs_c": "n.848-3224_848-3221delCTTT",
          "hgvs_p": null,
          "intron_rank": 6,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000666407.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2262,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 7,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "NR_015392.1",
          "gene_hgnc_id": 48669,
          "gene_symbol": "WEE2-AS1",
          "hgvs_c": "n.843-3224_843-3221delCTTT",
          "hgvs_p": null,
          "intron_rank": 6,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "NR_015392.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2864,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 5,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "NR_199840.1",
          "gene_hgnc_id": 48669,
          "gene_symbol": "WEE2-AS1",
          "hgvs_c": "n.1110-3224_1110-3221delCTTT",
          "hgvs_p": null,
          "intron_rank": 4,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "NR_199840.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2678,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 4,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "NR_199841.1",
          "gene_hgnc_id": 48669,
          "gene_symbol": "WEE2-AS1",
          "hgvs_c": "n.924-3224_924-3221delCTTT",
          "hgvs_p": null,
          "intron_rank": 3,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "NR_199841.1",
          "transcript_support_level": null
        }
      ],
      "custom_annotations": null,
      "dbscsnv_ada_prediction": null,
      "dbscsnv_ada_score": null,
      "dbsnp": "rs768323979",
      "effect": "frameshift_variant",
      "frequency_reference_population": 0.000058240035,
      "gene_hgnc_id": 19684,
      "gene_symbol": "WEE2",
      "gnomad_exomes_ac": 88,
      "gnomad_exomes_af": 0.0000601972,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_ac": 6,
      "gnomad_genomes_af": 0.0000394353,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_heteroplasmic": null,
      "gnomad_mito_homoplasmic": null,
      "hom_count_reference_population": 0,
      "mitotip_prediction": null,
      "mitotip_score": null,
      "pathogenicity_classification_combined": "Likely pathogenic",
      "phenotype_combined": "Oocyte maturation defect 5|WEE2-related disorder",
      "phylop100way_prediction": "Benign",
      "phylop100way_score": 0.007,
      "pos": 141708977,
      "ref": "CAAAG",
      "revel_prediction": null,
      "revel_score": null,
      "splice_prediction_selected": "Benign",
      "splice_score_selected": 0,
      "splice_source_selected": "max_spliceai",
      "spliceai_max_prediction": "Benign",
      "spliceai_max_score": 0,
      "transcript": "NM_001105558.1"
    }
  ]
}
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