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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 7-156676504-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=7&pos=156676504&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "7",
      "pos": 156676504,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_030936.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His",
          "transcript": "NM_030936.4",
          "protein_id": "NP_112198.1",
          "transcript_support_level": null,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 938,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000317955.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_030936.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His",
          "transcript": "ENST00000317955.10",
          "protein_id": "ENSP00000315950.5",
          "transcript_support_level": 1,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 938,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_030936.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000317955.10"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His",
          "transcript": "ENST00000392743.6",
          "protein_id": "ENSP00000376499.2",
          "transcript_support_level": 1,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 938,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000392743.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His",
          "transcript": "ENST00000432459.6",
          "protein_id": "ENSP00000405588.2",
          "transcript_support_level": 1,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 938,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000432459.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.*129G>A",
          "hgvs_p": null,
          "transcript": "ENST00000311822.12",
          "protein_id": "ENSP00000308894.8",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 310,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 933,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000311822.12"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His",
          "transcript": "NM_001184996.2",
          "protein_id": "NP_001171925.1",
          "transcript_support_level": null,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 938,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001184996.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His",
          "transcript": "NM_001184997.1",
          "protein_id": "NP_001171926.1",
          "transcript_support_level": null,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 938,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001184997.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His",
          "transcript": "ENST00000405335.5",
          "protein_id": "ENSP00000385285.1",
          "transcript_support_level": 5,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 938,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000405335.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His",
          "transcript": "ENST00000886327.1",
          "protein_id": "ENSP00000556386.1",
          "transcript_support_level": null,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 938,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000886327.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His",
          "transcript": "ENST00000886330.1",
          "protein_id": "ENSP00000556389.1",
          "transcript_support_level": null,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 938,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His",
          "transcript": "ENST00000886332.1",
          "protein_id": "ENSP00000556391.1",
          "transcript_support_level": null,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 938,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        {
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          "consequences": [
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          ],
          "exon_rank": 9,
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          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "RNF32",
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          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His",
          "transcript": "ENST00000886333.1",
          "protein_id": "ENSP00000556392.1",
          "transcript_support_level": null,
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          "cds_start": 938,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His",
          "transcript": "ENST00000886334.1",
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          "biotype": "protein_coding",
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        {
          "aa_ref": "R",
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          "protein_coding": true,
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          "consequences": [
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          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.899G>A",
          "hgvs_p": "p.Arg300His",
          "transcript": "ENST00000886328.1",
          "protein_id": "ENSP00000556387.1",
          "transcript_support_level": null,
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          "cds_start": 899,
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          "cdna_start": null,
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        {
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          "cds_start": 899,
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        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
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          ],
          "exon_rank": 9,
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          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.899G>A",
          "hgvs_p": "p.Arg300His",
          "transcript": "ENST00000972044.1",
          "protein_id": "ENSP00000642103.1",
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          "cds_start": 899,
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        {
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          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.899G>A",
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          "transcript": "ENST00000972045.1",
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        {
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          "gene_symbol": "RNF32",
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          "hgvs_p": "p.Arg293His",
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        {
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        },
        {
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          "strand": true,
          "consequences": [
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          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His",
          "transcript": "XM_011515804.4",
          "protein_id": "XP_011514106.1",
          "transcript_support_level": null,
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        {
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          "gene_symbol": "LMBR1",
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          "biotype": "pseudogene",
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        {
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          "consequences": [
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          "exon_count": 16,
          "intron_rank": null,
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          "gene_symbol": "LMBR1",
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          "hgvs_c": "n.3913C>T",
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          "transcript": "XR_007060137.1",
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          "biotype": "pseudogene",
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        },
        {
          "aa_ref": null,
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          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
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          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "RNF32",
          "gene_hgnc_id": 17118,
          "hgvs_c": "n.*757G>A",
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          "transcript": "ENST00000392747.6",
          "protein_id": "ENSP00000376503.2",
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000392747.6"
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      ],
      "gene_symbol": "RNF32",
      "gene_hgnc_id": 17118,
      "dbsnp": "rs770700325",
      "frequency_reference_population": 0.000016110003,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 26,
      "gnomad_exomes_af": 0.0000143669,
      "gnomad_genomes_af": 0.0000328502,
      "gnomad_exomes_ac": 21,
      "gnomad_genomes_ac": 5,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.041862159967422485,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.075,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0916,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.64,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -1.465,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_030936.4",
          "gene_symbol": "RNF32",
          "hgnc_id": 17118,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.938G>A",
          "hgvs_p": "p.Arg313His"
        },
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000609081.1",
          "gene_symbol": "LMBR1",
          "hgnc_id": 13243,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "n.213C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}