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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 7-27143364-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=7&pos=27143364&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "message": null,
  "variants": [
    {
      "acmg_by_gene": [
        {
          "benign_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "effects": [
            "missense_variant"
          ],
          "gene_symbol": "HOXA5",
          "hgnc_id": 5106,
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Ala82Thr",
          "inheritance_mode": "AD",
          "pathogenic_score": 2,
          "score": 0,
          "transcript": "NM_019102.4",
          "verdict": "Uncertain_significance"
        },
        {
          "benign_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "effects": [
            "intron_variant"
          ],
          "gene_symbol": "HOXA3",
          "hgnc_id": 5104,
          "hgvs_c": "c.-493-3178G>A",
          "hgvs_p": null,
          "inheritance_mode": "AD",
          "pathogenic_score": 2,
          "score": 0,
          "transcript": "NM_153631.3",
          "verdict": "Uncertain_significance"
        },
        {
          "benign_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "effects": [
            "intron_variant"
          ],
          "gene_symbol": "HOXA-AS3",
          "hgnc_id": 43748,
          "hgvs_c": "n.173-8211C>T",
          "hgvs_p": null,
          "inheritance_mode": "",
          "pathogenic_score": 2,
          "score": 0,
          "transcript": "ENST00000518848.5",
          "verdict": "Uncertain_significance"
        },
        {
          "benign_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "effects": [
            "intron_variant"
          ],
          "gene_symbol": "ENSG00000273433",
          "hgnc_id": null,
          "hgvs_c": "n.275-3178G>A",
          "hgvs_p": null,
          "inheritance_mode": "",
          "pathogenic_score": 2,
          "score": 0,
          "transcript": "ENST00000467897.2",
          "verdict": "Uncertain_significance"
        }
      ],
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_score": 0,
      "allele_count_reference_population": 4,
      "alphamissense_prediction": "Benign",
      "alphamissense_score": 0.0694,
      "alt": "T",
      "apogee2_prediction": null,
      "apogee2_score": null,
      "bayesdelnoaf_prediction": "Benign",
      "bayesdelnoaf_score": -0.38,
      "chr": "7",
      "clinvar_classification": "",
      "clinvar_disease": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "computational_prediction_selected": "Benign",
      "computational_score_selected": 0.18620029091835022,
      "computational_source_selected": "MetaRNN",
      "consequences": [
        {
          "aa_alt": "T",
          "aa_end": null,
          "aa_length": 270,
          "aa_ref": "A",
          "aa_start": 82,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1670,
          "cdna_start": 318,
          "cds_end": null,
          "cds_length": 813,
          "cds_start": 244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 2,
          "exon_rank": 1,
          "exon_rank_end": null,
          "feature": "NM_019102.4",
          "gene_hgnc_id": 5106,
          "gene_symbol": "HOXA5",
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Ala82Thr",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "ENST00000222726.4",
          "protein_coding": true,
          "protein_id": "NP_061975.2",
          "strand": false,
          "transcript": "NM_019102.4",
          "transcript_support_level": null
        },
        {
          "aa_alt": "T",
          "aa_end": null,
          "aa_length": 270,
          "aa_ref": "A",
          "aa_start": 82,
          "biotype": "protein_coding",
          "canonical": true,
          "cdna_end": null,
          "cdna_length": 1670,
          "cdna_start": 318,
          "cds_end": null,
          "cds_length": 813,
          "cds_start": 244,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 2,
          "exon_rank": 1,
          "exon_rank_end": null,
          "feature": "ENST00000222726.4",
          "gene_hgnc_id": 5106,
          "gene_symbol": "HOXA5",
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Ala82Thr",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "NM_019102.4",
          "protein_coding": true,
          "protein_id": "ENSP00000222726.3",
          "strand": false,
          "transcript": "ENST00000222726.4",
          "transcript_support_level": 1
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 443,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3026,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 1332,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 6,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "NM_153631.3",
          "gene_hgnc_id": 5104,
          "gene_symbol": "HOXA3",
          "hgvs_c": "c.-493-3178G>A",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "ENST00000612286.5",
          "protein_coding": true,
          "protein_id": "NP_705895.1",
          "strand": false,
          "transcript": "NM_153631.3",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 443,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": true,
          "cdna_end": null,
          "cdna_length": 3026,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 1332,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 6,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000612286.5",
          "gene_hgnc_id": 5104,
          "gene_symbol": "HOXA3",
          "hgvs_c": "c.-493-3178G>A",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "NM_153631.3",
          "protein_coding": true,
          "protein_id": "ENSP00000484411.1",
          "strand": false,
          "transcript": "ENST00000612286.5",
          "transcript_support_level": 2
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 443,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3142,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 1332,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 7,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "NM_001384335.1",
          "gene_hgnc_id": 5104,
          "gene_symbol": "HOXA3",
          "hgvs_c": "c.-609-3178G>A",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001371264.1",
          "strand": false,
          "transcript": "NM_001384335.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 443,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3188,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 1332,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 5,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "NM_001384336.1",
          "gene_hgnc_id": 5104,
          "gene_symbol": "HOXA3",
          "hgvs_c": "c.-308-3178G>A",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001371265.1",
          "strand": false,
          "transcript": "NM_001384336.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 443,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2868,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 1332,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 5,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000851228.1",
          "gene_hgnc_id": 5104,
          "gene_symbol": "HOXA3",
          "hgvs_c": "c.-308-3178G>A",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000521287.1",
          "strand": false,
          "transcript": "ENST00000851228.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 443,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2861,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 1332,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 7,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000851229.1",
          "gene_hgnc_id": 5104,
          "gene_symbol": "HOXA3",
          "hgvs_c": "c.-609-3178G>A",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000521288.1",
          "strand": false,
          "transcript": "ENST00000851229.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 443,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2887,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 1332,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 5,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000956341.1",
          "gene_hgnc_id": 5104,
          "gene_symbol": "HOXA3",
          "hgvs_c": "c.-308-3178G>A",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000626400.1",
          "strand": false,
          "transcript": "ENST00000956341.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 919,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 3,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000467897.2",
          "gene_hgnc_id": null,
          "gene_symbol": "ENSG00000273433",
          "hgvs_c": "n.275-3178G>A",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000467897.2",
          "transcript_support_level": 5
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 562,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 3,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000518848.5",
          "gene_hgnc_id": 43748,
          "gene_symbol": "HOXA-AS3",
          "hgvs_c": "n.173-8211C>T",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "ENST00000518848.5",
          "transcript_support_level": 4
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 687,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 3,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000521197.5",
          "gene_hgnc_id": 43748,
          "gene_symbol": "HOXA-AS3",
          "hgvs_c": "n.176+2825C>T",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "ENST00000521197.5",
          "transcript_support_level": 4
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3918,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 3,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "NR_038832.1",
          "gene_hgnc_id": 43748,
          "gene_symbol": "HOXA-AS3",
          "hgvs_c": "n.176+2825C>T",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "NR_038832.1",
          "transcript_support_level": null
        }
      ],
      "custom_annotations": null,
      "dbscsnv_ada_prediction": null,
      "dbscsnv_ada_score": null,
      "dbsnp": "rs776598451",
      "effect": "missense_variant",
      "frequency_reference_population": 0.0000027949511,
      "gene_hgnc_id": 5106,
      "gene_symbol": "HOXA5",
      "gnomad_exomes_ac": 4,
      "gnomad_exomes_af": 0.00000279495,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_ac": null,
      "gnomad_genomes_af": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_heteroplasmic": null,
      "gnomad_mito_homoplasmic": null,
      "hom_count_reference_population": 0,
      "mitotip_prediction": null,
      "mitotip_score": null,
      "pathogenicity_classification_combined": null,
      "phenotype_combined": null,
      "phylop100way_prediction": "Benign",
      "phylop100way_score": 2.297,
      "pos": 27143364,
      "ref": "C",
      "revel_prediction": "Benign",
      "revel_score": 0.225,
      "splice_prediction_selected": "Benign",
      "splice_score_selected": 0.05000000074505806,
      "splice_source_selected": "max_spliceai",
      "spliceai_max_prediction": "Benign",
      "spliceai_max_score": 0.05,
      "transcript": "NM_019102.4"
    }
  ]
}
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