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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 7-41965250-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=7&pos=41965250&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "7",
      "pos": 41965250,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_000168.6",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3823G>A",
          "hgvs_p": "p.Gly1275Arg",
          "transcript": "NM_000168.6",
          "protein_id": "NP_000159.3",
          "transcript_support_level": null,
          "aa_start": 1275,
          "aa_end": null,
          "aa_length": 1580,
          "cds_start": 3823,
          "cds_end": null,
          "cds_length": 4743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000395925.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000168.6"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3823G>A",
          "hgvs_p": "p.Gly1275Arg",
          "transcript": "ENST00000395925.8",
          "protein_id": "ENSP00000379258.3",
          "transcript_support_level": 5,
          "aa_start": 1275,
          "aa_end": null,
          "aa_length": 1580,
          "cds_start": 3823,
          "cds_end": null,
          "cds_length": 4743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000168.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395925.8"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3823G>A",
          "hgvs_p": "p.Gly1275Arg",
          "transcript": "ENST00000677605.1",
          "protein_id": "ENSP00000503743.1",
          "transcript_support_level": null,
          "aa_start": 1275,
          "aa_end": null,
          "aa_length": 1580,
          "cds_start": 3823,
          "cds_end": null,
          "cds_length": 4743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000677605.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3823G>A",
          "hgvs_p": "p.Gly1275Arg",
          "transcript": "ENST00000678429.1",
          "protein_id": "ENSP00000502957.1",
          "transcript_support_level": null,
          "aa_start": 1275,
          "aa_end": null,
          "aa_length": 1580,
          "cds_start": 3823,
          "cds_end": null,
          "cds_length": 4743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000678429.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3823G>A",
          "hgvs_p": "p.Gly1275Arg",
          "transcript": "ENST00000878750.1",
          "protein_id": "ENSP00000548809.1",
          "transcript_support_level": null,
          "aa_start": 1275,
          "aa_end": null,
          "aa_length": 1580,
          "cds_start": 3823,
          "cds_end": null,
          "cds_length": 4743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878750.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3823G>A",
          "hgvs_p": "p.Gly1275Arg",
          "transcript": "ENST00000878751.1",
          "protein_id": "ENSP00000548810.1",
          "transcript_support_level": null,
          "aa_start": 1275,
          "aa_end": null,
          "aa_length": 1580,
          "cds_start": 3823,
          "cds_end": null,
          "cds_length": 4743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878751.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3793G>A",
          "hgvs_p": "p.Gly1265Arg",
          "transcript": "ENST00000911414.1",
          "protein_id": "ENSP00000581473.1",
          "transcript_support_level": null,
          "aa_start": 1265,
          "aa_end": null,
          "aa_length": 1570,
          "cds_start": 3793,
          "cds_end": null,
          "cds_length": 4713,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000911414.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3649G>A",
          "hgvs_p": "p.Gly1217Arg",
          "transcript": "ENST00000677288.1",
          "protein_id": "ENSP00000503986.1",
          "transcript_support_level": null,
          "aa_start": 1217,
          "aa_end": null,
          "aa_length": 1522,
          "cds_start": 3649,
          "cds_end": null,
          "cds_length": 4569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000677288.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3646G>A",
          "hgvs_p": "p.Gly1216Arg",
          "transcript": "ENST00000479210.1",
          "protein_id": "ENSP00000496377.1",
          "transcript_support_level": 2,
          "aa_start": 1216,
          "aa_end": null,
          "aa_length": 1521,
          "cds_start": 3646,
          "cds_end": null,
          "cds_length": 4566,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000479210.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3823G>A",
          "hgvs_p": "p.Gly1275Arg",
          "transcript": "XM_047420205.1",
          "protein_id": "XP_047276161.1",
          "transcript_support_level": null,
          "aa_start": 1275,
          "aa_end": null,
          "aa_length": 1580,
          "cds_start": 3823,
          "cds_end": null,
          "cds_length": 4743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047420205.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3823G>A",
          "hgvs_p": "p.Gly1275Arg",
          "transcript": "XM_047420206.1",
          "protein_id": "XP_047276162.1",
          "transcript_support_level": null,
          "aa_start": 1275,
          "aa_end": null,
          "aa_length": 1580,
          "cds_start": 3823,
          "cds_end": null,
          "cds_length": 4743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047420206.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3823G>A",
          "hgvs_p": "p.Gly1275Arg",
          "transcript": "XM_047420207.1",
          "protein_id": "XP_047276163.1",
          "transcript_support_level": null,
          "aa_start": 1275,
          "aa_end": null,
          "aa_length": 1580,
          "cds_start": 3823,
          "cds_end": null,
          "cds_length": 4743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047420207.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3823G>A",
          "hgvs_p": "p.Gly1275Arg",
          "transcript": "XM_047420208.1",
          "protein_id": "XP_047276164.1",
          "transcript_support_level": null,
          "aa_start": 1275,
          "aa_end": null,
          "aa_length": 1580,
          "cds_start": 3823,
          "cds_end": null,
          "cds_length": 4743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047420208.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3823G>A",
          "hgvs_p": "p.Gly1275Arg",
          "transcript": "XM_047420209.1",
          "protein_id": "XP_047276165.1",
          "transcript_support_level": null,
          "aa_start": 1275,
          "aa_end": null,
          "aa_length": 1580,
          "cds_start": 3823,
          "cds_end": null,
          "cds_length": 4743,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047420209.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3820G>A",
          "hgvs_p": "p.Gly1274Arg",
          "transcript": "XM_017011997.2",
          "protein_id": "XP_016867486.1",
          "transcript_support_level": null,
          "aa_start": 1274,
          "aa_end": null,
          "aa_length": 1579,
          "cds_start": 3820,
          "cds_end": null,
          "cds_length": 4740,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017011997.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GLI3",
          "gene_hgnc_id": 4319,
          "hgvs_c": "c.3646G>A",
          "hgvs_p": "p.Gly1216Arg",
          "transcript": "XM_011515274.3",
          "protein_id": "XP_011513576.1",
          "transcript_support_level": null,
          "aa_start": 1216,
          "aa_end": null,
          "aa_length": 1521,
          "cds_start": 3646,
          "cds_end": null,
          "cds_length": 4566,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011515274.3"
        }
      ],
      "gene_symbol": "GLI3",
      "gene_hgnc_id": 4319,
      "dbsnp": "rs756156901",
      "frequency_reference_population": 0.000028055634,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 41,
      "gnomad_exomes_af": 0.0000280556,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 41,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.13049525022506714,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.121,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1485,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.45,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.719,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -3,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate,BP6",
      "acmg_by_gene": [
        {
          "score": -3,
          "benign_score": 3,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_000168.6",
          "gene_symbol": "GLI3",
          "hgnc_id": 4319,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.3823G>A",
          "hgvs_p": "p.Gly1275Arg"
        }
      ],
      "clinvar_disease": " postaxial, type A1,Greig cephalopolysyndactyly syndrome,Inborn genetic diseases,Pallister-Hall syndrome,Polydactyly,Polysyndactyly 4",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:2 LB:1",
      "phenotype_combined": "Pallister-Hall syndrome;Greig cephalopolysyndactyly syndrome|Pallister-Hall syndrome;Greig cephalopolysyndactyly syndrome;Polysyndactyly 4;Polydactyly, postaxial, type A1|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}