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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 7-65961022-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=7&pos=65961022&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "7",
      "pos": 65961022,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000304895.9",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "c.1831C>T",
          "hgvs_p": "p.Arg611Trp",
          "transcript": "NM_000181.4",
          "protein_id": "NP_000172.2",
          "transcript_support_level": null,
          "aa_start": 611,
          "aa_end": null,
          "aa_length": 651,
          "cds_start": 1831,
          "cds_end": null,
          "cds_length": 1956,
          "cdna_start": 1861,
          "cdna_end": null,
          "cdna_length": 2199,
          "mane_select": "ENST00000304895.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "c.1831C>T",
          "hgvs_p": "p.Arg611Trp",
          "transcript": "ENST00000304895.9",
          "protein_id": "ENSP00000302728.4",
          "transcript_support_level": 1,
          "aa_start": 611,
          "aa_end": null,
          "aa_length": 651,
          "cds_start": 1831,
          "cds_end": null,
          "cds_length": 1956,
          "cdna_start": 1861,
          "cdna_end": null,
          "cdna_length": 2199,
          "mane_select": "NM_000181.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "c.1393C>T",
          "hgvs_p": "p.Arg465Trp",
          "transcript": "NM_001284290.2",
          "protein_id": "NP_001271219.1",
          "transcript_support_level": null,
          "aa_start": 465,
          "aa_end": null,
          "aa_length": 505,
          "cds_start": 1393,
          "cds_end": null,
          "cds_length": 1518,
          "cdna_start": 1423,
          "cdna_end": null,
          "cdna_length": 1761,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "c.1393C>T",
          "hgvs_p": "p.Arg465Trp",
          "transcript": "ENST00000421103.5",
          "protein_id": "ENSP00000391390.1",
          "transcript_support_level": 2,
          "aa_start": 465,
          "aa_end": null,
          "aa_length": 505,
          "cds_start": 1393,
          "cds_end": null,
          "cds_length": 1518,
          "cdna_start": 1421,
          "cdna_end": null,
          "cdna_length": 1742,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "c.1261C>T",
          "hgvs_p": "p.Arg421Trp",
          "transcript": "NM_001293104.2",
          "protein_id": "NP_001280033.1",
          "transcript_support_level": null,
          "aa_start": 421,
          "aa_end": null,
          "aa_length": 461,
          "cds_start": 1261,
          "cds_end": null,
          "cds_length": 1386,
          "cdna_start": 1676,
          "cdna_end": null,
          "cdna_length": 2014,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "c.1174C>T",
          "hgvs_p": "p.Arg392Trp",
          "transcript": "NM_001293105.2",
          "protein_id": "NP_001280034.1",
          "transcript_support_level": null,
          "aa_start": 392,
          "aa_end": null,
          "aa_length": 432,
          "cds_start": 1174,
          "cds_end": null,
          "cds_length": 1299,
          "cdna_start": 1533,
          "cdna_end": null,
          "cdna_length": 1871,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "c.1678C>T",
          "hgvs_p": "p.Arg560Trp",
          "transcript": "XM_005250297.5",
          "protein_id": "XP_005250354.1",
          "transcript_support_level": null,
          "aa_start": 560,
          "aa_end": null,
          "aa_length": 600,
          "cds_start": 1678,
          "cds_end": null,
          "cds_length": 1803,
          "cdna_start": 1708,
          "cdna_end": null,
          "cdna_length": 2046,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "c.1330C>T",
          "hgvs_p": "p.Arg444Trp",
          "transcript": "XM_047420286.1",
          "protein_id": "XP_047276242.1",
          "transcript_support_level": null,
          "aa_start": 444,
          "aa_end": null,
          "aa_length": 484,
          "cds_start": 1330,
          "cds_end": null,
          "cds_length": 1455,
          "cdna_start": 1449,
          "cdna_end": null,
          "cdna_length": 1787,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "c.1177C>T",
          "hgvs_p": "p.Arg393Trp",
          "transcript": "XM_017012091.2",
          "protein_id": "XP_016867580.1",
          "transcript_support_level": null,
          "aa_start": 393,
          "aa_end": null,
          "aa_length": 433,
          "cds_start": 1177,
          "cds_end": null,
          "cds_length": 1302,
          "cdna_start": 1275,
          "cdna_end": null,
          "cdna_length": 1613,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "c.1108C>T",
          "hgvs_p": "p.Arg370Trp",
          "transcript": "XM_047420288.1",
          "protein_id": "XP_047276244.1",
          "transcript_support_level": null,
          "aa_start": 370,
          "aa_end": null,
          "aa_length": 410,
          "cds_start": 1108,
          "cds_end": null,
          "cds_length": 1233,
          "cdna_start": 1266,
          "cdna_end": null,
          "cdna_length": 1604,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "c.1021C>T",
          "hgvs_p": "p.Arg341Trp",
          "transcript": "XM_047420289.1",
          "protein_id": "XP_047276245.1",
          "transcript_support_level": null,
          "aa_start": 341,
          "aa_end": null,
          "aa_length": 381,
          "cds_start": 1021,
          "cds_end": null,
          "cds_length": 1146,
          "cdna_start": 1140,
          "cdna_end": null,
          "cdna_length": 1478,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "n.*1098C>T",
          "hgvs_p": null,
          "transcript": "ENST00000430730.5",
          "protein_id": "ENSP00000411859.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1859,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "n.*1211C>T",
          "hgvs_p": null,
          "transcript": "ENST00000447929.5",
          "protein_id": "ENSP00000411262.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1983,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "n.2221C>T",
          "hgvs_p": null,
          "transcript": "ENST00000466883.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2555,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "n.1776C>T",
          "hgvs_p": null,
          "transcript": "NR_120531.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2114,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "n.*1098C>T",
          "hgvs_p": null,
          "transcript": "ENST00000430730.5",
          "protein_id": "ENSP00000411859.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1859,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GUSB",
          "gene_hgnc_id": 4696,
          "hgvs_c": "n.*1211C>T",
          "hgvs_p": null,
          "transcript": "ENST00000447929.5",
          "protein_id": "ENSP00000411262.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1983,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "GUSB",
      "gene_hgnc_id": 4696,
      "dbsnp": "rs121918176",
      "frequency_reference_population": 0.0000049611726,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 8,
      "gnomad_exomes_af": 0.00000478891,
      "gnomad_genomes_af": 0.00000663086,
      "gnomad_exomes_ac": 7,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9667918086051941,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.89,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.8165,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.53,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 4.053,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 9,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PM5,PP3_Strong,PP5",
      "acmg_by_gene": [
        {
          "score": 9,
          "benign_score": 0,
          "pathogenic_score": 9,
          "criteria": [
            "PM2",
            "PM5",
            "PP3_Strong",
            "PP5"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000304895.9",
          "gene_symbol": "GUSB",
          "hgnc_id": 4696,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1831C>T",
          "hgvs_p": "p.Arg611Trp"
        }
      ],
      "clinvar_disease": "Mucopolysaccharidosis type 7",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "LP:2 US:1",
      "phenotype_combined": "Mucopolysaccharidosis type 7",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}