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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 8-101689356-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=8&pos=101689356&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "8",
      "pos": 101689356,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_032041.3",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "NM_032041.3",
          "protein_id": "NP_114430.2",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000220931.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_032041.3"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "ENST00000220931.11",
          "protein_id": "ENSP00000220931.6",
          "transcript_support_level": 1,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_032041.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000220931.11"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "ENST00000519508.6",
          "protein_id": "ENSP00000430476.1",
          "transcript_support_level": 1,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000519508.6"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "ENST00000521599.5",
          "protein_id": "ENSP00000428105.1",
          "transcript_support_level": 1,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000521599.5"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "NM_001040624.2",
          "protein_id": "NP_001035714.1",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001040624.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "NM_001040625.2",
          "protein_id": "NP_001035715.1",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001040625.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "NM_001040626.2",
          "protein_id": "NP_001035716.1",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001040626.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "NM_001040627.2",
          "protein_id": "NP_001035717.1",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001040627.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "NM_001040628.2",
          "protein_id": "NP_001035718.1",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001040628.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "NM_001040629.2",
          "protein_id": "NP_001035719.1",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001040629.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "NM_001040630.2",
          "protein_id": "NP_001035720.1",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001040630.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "ENST00000311028.4",
          "protein_id": "ENSP00000310587.3",
          "transcript_support_level": 5,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000311028.4"
        },
        {
          "aa_ref": "I",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "ENST00000395923.5",
          "protein_id": "ENSP00000379256.1",
          "transcript_support_level": 5,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
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          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395923.5"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "XM_047422303.1",
          "protein_id": "XP_047278259.1",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
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          "cds_length": 582,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "I",
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          "strand": false,
          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "NCALD",
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          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "XM_047422304.1",
          "protein_id": "XP_047278260.1",
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          "cds_start": 535,
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          "cds_length": 582,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047422304.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "XM_047422305.1",
          "protein_id": "XP_047278261.1",
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          "aa_end": null,
          "aa_length": 193,
          "cds_start": 535,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "XM_047422306.1",
          "protein_id": "XP_047278262.1",
          "transcript_support_level": null,
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          "cds_start": 535,
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        {
          "aa_ref": "I",
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          ],
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          "intron_rank": null,
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          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
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          "transcript": "XM_047422307.1",
          "protein_id": "XP_047278263.1",
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          "cds_start": 535,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "I",
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          "protein_coding": true,
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          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
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          "transcript": "XM_047422308.1",
          "protein_id": "XP_047278264.1",
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_047422308.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCALD",
          "gene_hgnc_id": 7655,
          "hgvs_c": "c.535A>G",
          "hgvs_p": "p.Ile179Val",
          "transcript": "XM_047422309.1",
          "protein_id": "XP_047278265.1",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
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          "cds_start": 535,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_047422309.1"
        },
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      "dbsnp": "rs1336690615",
      "frequency_reference_population": 0.0000018595817,
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      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000136888,
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      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.2934234142303467,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.217,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.5188,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.14,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 8.012,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
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          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
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            "BP4"
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          "verdict": "Uncertain_significance",
          "transcript": "NM_032041.3",
          "gene_symbol": "NCALD",
          "hgnc_id": 7655,
          "effects": [
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          "inheritance_mode": "",
          "hgvs_c": "c.535A>G",
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}