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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 8-103403276-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=8&pos=103403276&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "8",
      "pos": 103403276,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000297578.9",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC25A32",
          "gene_hgnc_id": 29683,
          "hgvs_c": "c.440G>A",
          "hgvs_p": "p.Arg147His",
          "transcript": "NM_030780.5",
          "protein_id": "NP_110407.2",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 315,
          "cds_start": 440,
          "cds_end": null,
          "cds_length": 948,
          "cdna_start": 610,
          "cdna_end": null,
          "cdna_length": 2891,
          "mane_select": "ENST00000297578.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC25A32",
          "gene_hgnc_id": 29683,
          "hgvs_c": "c.440G>A",
          "hgvs_p": "p.Arg147His",
          "transcript": "ENST00000297578.9",
          "protein_id": "ENSP00000297578.4",
          "transcript_support_level": 1,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 315,
          "cds_start": 440,
          "cds_end": null,
          "cds_length": 948,
          "cdna_start": 610,
          "cdna_end": null,
          "cdna_length": 2891,
          "mane_select": "NM_030780.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000285982",
          "gene_hgnc_id": null,
          "hgvs_c": "c.287G>A",
          "hgvs_p": "p.Arg96His",
          "transcript": "ENST00000649416.1",
          "protein_id": "ENSP00000496817.1",
          "transcript_support_level": null,
          "aa_start": 96,
          "aa_end": null,
          "aa_length": 264,
          "cds_start": 287,
          "cds_end": null,
          "cds_length": 795,
          "cdna_start": 618,
          "cdna_end": null,
          "cdna_length": 2879,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC25A32",
          "gene_hgnc_id": 29683,
          "hgvs_c": "c.509G>A",
          "hgvs_p": "p.Arg170His",
          "transcript": "ENST00000707124.1",
          "protein_id": "ENSP00000516752.1",
          "transcript_support_level": null,
          "aa_start": 170,
          "aa_end": null,
          "aa_length": 338,
          "cds_start": 509,
          "cds_end": null,
          "cds_length": 1017,
          "cdna_start": 610,
          "cdna_end": null,
          "cdna_length": 2891,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC25A32",
          "gene_hgnc_id": 29683,
          "hgvs_c": "n.354G>A",
          "hgvs_p": null,
          "transcript": "ENST00000523866.1",
          "protein_id": "ENSP00000430371.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1347,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC25A32",
          "gene_hgnc_id": 29683,
          "hgvs_c": "n.524G>A",
          "hgvs_p": null,
          "transcript": "NR_102337.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2805,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC25A32",
          "gene_hgnc_id": 29683,
          "hgvs_c": "n.719G>A",
          "hgvs_p": null,
          "transcript": "NR_102338.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3000,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "SLC25A32",
          "gene_hgnc_id": 29683,
          "hgvs_c": "n.392-1222G>A",
          "hgvs_p": null,
          "transcript": "ENST00000521645.5",
          "protein_id": "ENSP00000430989.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2815,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SLC25A32",
          "gene_hgnc_id": 29683,
          "hgvs_c": "n.306-1222G>A",
          "hgvs_p": null,
          "transcript": "ENST00000523256.6",
          "protein_id": "ENSP00000427737.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2711,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "SLC25A32",
      "gene_hgnc_id": 29683,
      "dbsnp": "rs142329098",
      "frequency_reference_population": 0.00017119039,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 276,
      "gnomad_exomes_af": 0.000167772,
      "gnomad_genomes_af": 0.000204047,
      "gnomad_exomes_ac": 245,
      "gnomad_genomes_ac": 31,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8229525685310364,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.05999999865889549,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.885,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.893,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.41,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 5.559,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.06,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP3,PP5",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PP3",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000297578.9",
          "gene_symbol": "SLC25A32",
          "hgnc_id": 29683,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,Unknown",
          "hgvs_c": "c.440G>A",
          "hgvs_p": "p.Arg147His"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PP3",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000649416.1",
          "gene_symbol": "ENSG00000285982",
          "hgnc_id": null,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.287G>A",
          "hgvs_p": "p.Arg96His"
        }
      ],
      "clinvar_disease": " riboflavin-responsive,Exercise intolerance,not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "LP:1 US:2",
      "phenotype_combined": "Exercise intolerance, riboflavin-responsive|not provided|not specified",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}